IL-1 Beta antibody | 2E8
Mouse anti Human IL-1 beta, clone 2E8 recognizes human interleukin-1β, also known as catabolin. IL-1β is a 153 amino acid ~18 kDa with an additional 116 propeptide region, cleaved in the mature form. IL-1β is a potent proinflammatory cytokine released by activated macrophages as a ~35 proprotein which is then cleaved by caspase-1 to yield the active cytokine (Andrei et al. 2004).
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Immunogen
- Recombinant protein corresponding to aa 1-270 of human IL-1 beta.
- Approx. Protein Concentrations
- Ig concentration 0.5 mg/ml
- Fusion Partners
- Spleen cells from BALB/c mice were fused with cells from the Sp2/0 myeloma cell line.
Storage Information
- Storage
- Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
More Information
- UniProt
- P01584 Related reagents
- Entrez Gene
- IL1B Related reagents
- GO Terms
- GO:0000187 activation of MAPK activity
- GO:0001660 fever generation
- GO:0001934 positive regulation of protein phosphorylation
- GO:0002711 positive regulation of T cell mediated immunity
- GO:0006915 apoptosis
- GO:0005829 cytosol
- GO:0005125 cytokine activity
- GO:0005149 interleukin-1 receptor binding
- GO:0005615 extracellular space
- GO:0006916 anti-apoptosis
- GO:0007267 cell-cell signaling
- GO:0007566 embryo implantation
- GO:0050796 regulation of insulin secretion
- GO:0008083 growth factor activity
- GO:0008285 negative regulation of cell proliferation
- GO:0010575 positive regulation vascular endothelial growth factor production
- GO:0010829 negative regulation of glucose transport
- GO:0014805 smooth muscle adaptation
- GO:0019221 cytokine-mediated signaling pathway
- GO:0019904 protein domain specific binding
- GO:0030730 sequestering of triglyceride
- GO:0031622 positive regulation of fever generation
- GO:0032308 positive regulation of prostaglandin secretion
- GO:0032725 positive regulation of granulocyte macrophage colony-stimulating factor production
- GO:0032729 positive regulation of interferon-gamma production
- GO:0032755 positive regulation of interleukin-6 production
- GO:0034116 positive regulation of heterotypic cell-cell adhesion
- GO:0035505 positive regulation of myosin light chain kinase activity
- GO:0042102 positive regulation of T cell proliferation
- GO:0042346 positive regulation of NF-kappaB import into nucleus
- GO:0043407 negative regulation of MAP kinase activity
- GO:0045086 positive regulation of interleukin-2 biosynthetic process
- GO:0045429 positive regulation of nitric oxide biosynthetic process
- GO:0045766 positive regulation of angiogenesis
- GO:0045840 positive regulation of mitosis
- GO:0046627 negative regulation of insulin receptor signaling pathway
- GO:0046827 positive regulation of protein export from nucleus
- GO:0050995 negative regulation of lipid catabolic process
- GO:0050996 positive regulation of lipid catabolic process
- GO:0051044 positive regulation of membrane protein ectodomain proteolysis
- GO:0051092 positive regulation of NF-kappaB transcription factor activity
- GO:0051781 positive regulation of cell division
- GO:0060355 positive regulation of cell adhesion molecule production
- GO:0060559 positive regulation of calcidiol 1-monooxygenase activity
- GO:0070164 negative regulation of adiponectin secretion
- Regulatory
- For research purposes only
Applications of IL-1 Beta antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunofluorescence | 0.1 | 10 ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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Secondary Antibodies Available
