IL-1 Beta antibody | 2E8

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Mouse anti Human IL-1 Beta:Preservative Free

Product Type
Monoclonal Antibody
Clone
2E8
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA5542Z IF 0.1 mg
Mouse anti Human IL-1 beta, clone 2E8 recognizes human interleukin-1β, also known as catabolin. IL-1β is a 153 amino acid ~18 kDa with an additional 116 propeptide region, cleaved in the mature form. IL-1β is a potent proinflammatory cytokine released by activated macrophages as a ~35 proprotein which is then cleaved by caspase-1 to yield the active cytokine (Andrei et al. 2004).

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Immunogen
Recombinant protein corresponding to aa 1-270 of human IL-1 beta.
Approx. Protein Concentrations
Ig concentration 0.5 mg/ml
Fusion Partners
Spleen cells from BALB/c mice were fused with cells from the Sp2/0 myeloma cell line.

Storage Information

Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P01584 Related reagents
Entrez Gene
IL1B Related reagents
GO Terms
GO:0000187 activation of MAPK activity
GO:0001660 fever generation
GO:0001934 positive regulation of protein phosphorylation
GO:0002711 positive regulation of T cell mediated immunity
GO:0006915 apoptosis
GO:0005829 cytosol
GO:0005125 cytokine activity
GO:0005149 interleukin-1 receptor binding
GO:0005615 extracellular space
GO:0006916 anti-apoptosis
GO:0007267 cell-cell signaling
GO:0007566 embryo implantation
GO:0050796 regulation of insulin secretion
GO:0008083 growth factor activity
GO:0008285 negative regulation of cell proliferation
GO:0010575 positive regulation vascular endothelial growth factor production
GO:0010829 negative regulation of glucose transport
GO:0014805 smooth muscle adaptation
GO:0019221 cytokine-mediated signaling pathway
GO:0019904 protein domain specific binding
GO:0030730 sequestering of triglyceride
GO:0031622 positive regulation of fever generation
GO:0032308 positive regulation of prostaglandin secretion
GO:0032725 positive regulation of granulocyte macrophage colony-stimulating factor production
GO:0032729 positive regulation of interferon-gamma production
GO:0032755 positive regulation of interleukin-6 production
GO:0034116 positive regulation of heterotypic cell-cell adhesion
GO:0035505 positive regulation of myosin light chain kinase activity
GO:0042102 positive regulation of T cell proliferation
GO:0042346 positive regulation of NF-kappaB import into nucleus
GO:0043407 negative regulation of MAP kinase activity
GO:0045086 positive regulation of interleukin-2 biosynthetic process
GO:0045429 positive regulation of nitric oxide biosynthetic process
GO:0045766 positive regulation of angiogenesis
GO:0045840 positive regulation of mitosis
GO:0046627 negative regulation of insulin receptor signaling pathway
GO:0046827 positive regulation of protein export from nucleus
GO:0050995 negative regulation of lipid catabolic process
GO:0050996 positive regulation of lipid catabolic process
GO:0051044 positive regulation of membrane protein ectodomain proteolysis
GO:0051092 positive regulation of NF-kappaB transcription factor activity
GO:0051781 positive regulation of cell division
GO:0060355 positive regulation of cell adhesion molecule production
GO:0060559 positive regulation of calcidiol 1-monooxygenase activity
GO:0070164 negative regulation of adiponectin secretion
Regulatory
For research purposes only

Applications of IL-1 Beta antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunofluorescence 0.1 10 ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F