IL-1 Beta antibody | 2E8

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Anti Human IL-1 Beta Antibody, clone 2E8 gallery image 1 — Immunofluorescence showing IL-1 Betain HeLa cells using Mouse anti Human IL-1 Beta (MCA5542Z)

Mouse anti Human IL-1 Beta:Preservative Free

Product Type: Monoclonal Antibody
Clone: 2E8
Isotype: IgG1

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
MCA5542Z	IF			0.1 mg

Summary
Secondary Antibodies

Mouse anti Human IL-1 beta, clone 2E8 recognizes human interleukin-1β, also known as catabolin. IL-1β is a 153 amino acid ~18 kDa with an additional 116 propeptide region, cleaved in the mature form. IL-1β is a potent proinflammatory cytokine released by activated macrophages as a ~35 proprotein which is then cleaved by caspase-1 to yield the active cytokine (Andrei et al. 2004).

Product Details

Target Species: Human
Product Form: Purified IgG - liquid
Preparation: Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: None present
Immunogen: Recombinant protein corresponding to aa 1-270 of human IL-1 beta.
Approx. Protein Concentrations: Ig concentration 0.5 mg/ml
Fusion Partners: Spleen cells from BALB/c mice were fused with cells from the Sp2/0 myeloma cell line.

Storage Information

Storage: Store at -20^oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

More Information

UniProt: P01584 Related reagents
Entrez Gene: IL1B Related reagents
GO Terms: GO:0000187 activation of MAPK activity; GO:0001660 fever generation; GO:0001934 positive regulation of protein phosphorylation; GO:0002711 positive regulation of T cell mediated immunity; GO:0006915 apoptosis; GO:0005829 cytosol; GO:0005125 cytokine activity; GO:0005149 interleukin-1 receptor binding; GO:0005615 extracellular space; GO:0006916 anti-apoptosis; GO:0007267 cell-cell signaling; GO:0007566 embryo implantation; GO:0050796 regulation of insulin secretion; GO:0008083 growth factor activity; GO:0008285 negative regulation of cell proliferation; GO:0010575 positive regulation vascular endothelial growth factor production; GO:0010829 negative regulation of glucose transport; GO:0014805 smooth muscle adaptation; GO:0019221 cytokine-mediated signaling pathway; GO:0019904 protein domain specific binding; GO:0030730 sequestering of triglyceride; GO:0031622 positive regulation of fever generation; GO:0032308 positive regulation of prostaglandin secretion; GO:0032725 positive regulation of granulocyte macrophage colony-stimulating factor production; GO:0032729 positive regulation of interferon-gamma production; GO:0032755 positive regulation of interleukin-6 production; GO:0034116 positive regulation of heterotypic cell-cell adhesion; GO:0035505 positive regulation of myosin light chain kinase activity; GO:0042102 positive regulation of T cell proliferation; GO:0042346 positive regulation of NF-kappaB import into nucleus; GO:0043407 negative regulation of MAP kinase activity; GO:0045086 positive regulation of interleukin-2 biosynthetic process; GO:0045429 positive regulation of nitric oxide biosynthetic process; GO:0045766 positive regulation of angiogenesis; GO:0045840 positive regulation of mitosis; GO:0046627 negative regulation of insulin receptor signaling pathway; GO:0046827 positive regulation of protein export from nucleus; GO:0050995 negative regulation of lipid catabolic process; GO:0050996 positive regulation of lipid catabolic process; GO:0051044 positive regulation of membrane protein ectodomain proteolysis; GO:0051092 positive regulation of NF-kappaB transcription factor activity; GO:0051781 positive regulation of cell division; GO:0060355 positive regulation of cell adhesion molecule production; GO:0060559 positive regulation of calcidiol 1-monooxygenase activity; GO:0070164 negative regulation of adiponectin secretion
Regulatory: For research purposes only

Applications of IL-1 Beta antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Immunofluorescence		0.1	10 ug/ml

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

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