IFN Gamma antibody | 8C4H9
Mouse anti Human IFN Gamma
- Product Type
- Monoclonal Antibody
- Clone
- 8C4H9
- Isotype
- IgG2b
- Specificity
- IFN Gamma
Mouse anti Human IFN gamma antibody, clone 8C4H9, recognizes interferon gamma (IFN gamma) also known as immune interferon, a type II interferon. It is a cytokine which is mainly expressed by CD4+ Th1 T lymphocytes and natural killer cells. IFN gamma plays many roles within immunity, including the activation of macrophages, T helper lymphocytes, stimulating antigen presentation, inflammation and leukocyte trafficking (Pereiro et al. 2019). After infection antigen presenting cells secrete IL-12, IL-18 and IL-1 beta which can promote IFN gamma synthesis (Schoenborn & Wilson 2007). IFN gamma can then elicit responses through interferon gamma receptor 1 and interferon gamma receptor 2. Activation of these receptors can then trigger immune responses through the JAK/STAT signaling pathway (Pereiro et al. 2019). Under physiological conditions, IFN gamma is constitutively expressed through a tightly controlled mechanism. However, it has been shown to have some protumorigenic roles, by upregulating checkpoint inhibitor programmed cell-death ligand 1. Conversely though, there have been some positive associations between IFN gamma and patient survival in several cancer types. As such, the exact roles that IFN gamma plays in cancer may largely be dependent on tumor specific context and microenvironmental cues (Castro et al. 2018). The purified Mouse anti Human IFN gamma antibody, clone 8C4H9 (MCA6238GA) can be used as a capture antibody in a sandwich ELISA with the biotinylated Mouse anti Human IFN gamma antibody, clone 18F11G6 (MCA6239B) as the detection antibody. |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- E. coli derived recombinant human full length IFN gamma (Gln24-Gln166)
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Cell fusion between immunized BALB/c mouse spleen cells and mouse myeloma SP2/0
- ELISA Matched Pair(s)
- ELISA Matched Pair-Detection Antibody: MCA6239B
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA | 1.0 ug/ml |
- UniProt
- P01579
- Entrez Gene
- IFNG
- GO Terms
- GO:0000060 protein import into nucleus, translocation
- GO:0002053 positive regulation of mesenchymal cell proliferation
- GO:0003340 negative regulation of mesenchymal to epithelial transition involved in metanephros morphogenesis
- GO:0009615 response to virus
- GO:0005125 cytokine activity
- GO:0005133 interferon-gamma receptor binding
- GO:0005615 extracellular space
- GO:0006955 immune response
- GO:0007050 cell cycle arrest
- View More GO Terms
- GO:0050796 regulation of insulin secretion
- GO:0010553 negative regulation of gene-specific transcription from RNA polymerase II promoter
- GO:0032735 positive regulation of interleukin-12 production
- GO:0032747 positive regulation of interleukin-23 production
- GO:0033141 positive regulation of peptidyl-serine phosphorylation of STAT protein
- GO:0034393 positive regulation of smooth muscle cell apoptosis
- GO:0042508 tyrosine phosphorylation of Stat1 protein
- GO:0042511 positive regulation of tyrosine phosphorylation of Stat1 protein
- GO:0045429 positive regulation of nitric oxide biosynthetic process
- GO:0045672 positive regulation of osteoclast differentiation
- GO:0048662 negative regulation of smooth muscle cell proliferation
- GO:0051044 positive regulation of membrane protein ectodomain proteolysis
- GO:0051712 positive regulation of killing of cells of other organism
- GO:0060333 interferon-gamma-mediated signaling pathway
- GO:0060334 regulation of interferon-gamma-mediated signaling pathway
- GO:0060550 positive regulation of fructose 1,6-bisphosphate 1-phosphatase activity
- GO:0060552 positive regulation of fructose 1,6-bisphosphate metabolic process
- GO:0060557 positive regulation of vitamin D biosynthetic process
- GO:0060559 positive regulation of calcidiol 1-monooxygenase activity
- GO:0072308 negative regulation of metanephric nephron tubule epithelial cell differentiation
MCA6238GA
100005442If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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