IFN Alpha antibody | C10F5

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Mouse anti Human Interferon Alpha

Product Type: Monoclonal Antibody
Clone: C10F5
Isotype: IgG1

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
MCA1117	C E			0.5 mg

Mouse anti Human interferon alpha antibody, clone C10F5 recognizes an epitope common to IFN-alpha subtypes A, F, D, I1 and P. Strongest reaction in ELISA is with subtypes alpha F and alpha D. It has an affinity constant of 2.4 x 10^-10M.

Mouse anti Human interferon alpha antibody, clone C10F5 has bee used successfully for the immunohistochemical detection of interferon-α producing cells on cryostat sections in a human suction blister model (Reed et al. 2004).

Product Details

Target Species

Human

Product Form

Purified IgG - liquid

Preparation

Purified IgG prepared by affinity chromatography on Protein A

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09%

Sodium Azide

Immunogen

Recombinant Human Interferon alpha -A.

Approx. Protein Concentrations

IgG concentration 1.0 mg/ml

Fusion Partners

Spleen cells from immunized BALB/c mice were fused with cells of the X63. Ag8.653 myeloma cell line.

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee: 18 months from date of despatch.

More Information

UniProt: P01562 Related reagents; P01568 Related reagents; P01563 Related reagents
Entrez Gene: IFNA13 Related reagents; IFNA2 Related reagents; IFNA21 Related reagents
GO Terms: GO:0009615 response to virus; GO:0007596 blood coagulation; GO:0005125 cytokine activity; GO:0005132 interferon-alpha/beta receptor binding; GO:0005615 extracellular space; GO:0006917 induction of apoptosis; GO:0006954 inflammatory response; GO:0007267 cell-cell signaling; GO:0060337 type I interferon-mediated signaling pathway; GO:0060338 regulation of type I interferon-mediated signaling pathway; GO:0006952 defense response; GO:0005126 cytokine receptor binding
Regulatory: For research purposes only

Applications of IFN Alpha antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA
Immunohistology - Frozen

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Application Based External Images

Immunohistology - Frozen

Product Specific References

References for IFN Alpha antibody

Reed, J.R. et al. (2004) Telomere erosion in memory T cells induced by telomerase inhibition at the site of antigenic challenge in vivo.
J Exp Med. 199 (10): 1433-43.
van der Laan, A. M. (2013) Adverse left ventricular remodelling after acute myocardial infarction in patients is associated with attenuated type I interferon signalling
In: Monocytes in ischemic heart disease ‘s-Hertogenbosch: Boxpress

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