hCG antibody | INN-hFSH-132

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Mouse anti hCG (Alpha 4 Epitope)

Product Type
Monoclonal Antibody
Clone
INN-hFSH-132
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA1026 E IP P* WB 0.5 mg
Mouse anti hCG (Alpha 4 Epitope) antibody, clone INN-hFSH-132 is a high affinity antibody recognising the alpha 4 epitope of the alpha-subunit of Human Chorionic Gonadotrophin (hCG), hLH, hFSH, hTSH and free alpha-subunit.

The recognised epitope comprises amino-acids alpha 15 to alpha 22 of the human sequence.

Mouse anti hCG (Alpha 4 Epitope) antibody, clone INN-hFSH-132 will not cross-react with glycoprotein hormones of various animal species.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by ion exchange chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
Human Follicle Stimulating Hormone (FSH).
Approx. Protein Concentrations
IgG concentration 1 mg/ml
Fusion Partners
Spleen cells from immunised mice were fused with cells of the X63-Ag8.653 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P01215 Related reagents
Entrez Gene
CGA Related reagents
GO Terms
GO:0005576 extracellular region
GO:0007165 signal transduction
GO:0005179 hormone activity
GO:0005625 soluble fraction
GO:0016486 peptide hormone processing
GO:0042446 hormone biosynthetic process
Regulatory
For research purposes only

Applications of hCG antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunohistology - Paraffin 1
Immunoprecipitation
Western Blotting
  1. 1 This product requires antigen retrieval using steam heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Application Based External Images

Western Blotting

Product Specific References

References for hCG antibody

  1. Berger, P. et al. (1988) Antigenic features of human follicle stimulating hormone delineated by monoclonal antibodies and construction of an immunoradiomometric assay.
    Endocrinology. 123 (5): 2351-9.
  2. Berger, P. et al. (1990) Monoclonal antibodies against the free subunits of human chorionic gonadotrophin.
    J Endocrinol. 125 (2): 301-9.
  3. Dirnhofer, S. et al. (1994) Free alpha subunit of human chorionic gonadotrophin: molecular basis of immunologically and biologically active domains.
    J Endocrinol. 140 (1): 145-54.
  4. Zimmermann, G. et al. (2009) Epithelial human chorionic gonadotropin is expressed and produced in human secretory endometrium during the normal menstrual cycle.
    Biol Reprod. 80:1053-65.
  5. Untergasser, G. et al. (2001) Seminal plasma factors induce in vitro PRL secretion in smooth muscle cells of the human prostate.
    J Clin Endocrinol Metab. 86: 5577-84.
  6. Zenzmaier, C. et al. (2011) Decreased levels of genuine large free hCG alpha in men presenting with abnormal semen analysis.
    Reprod Biol Endocrinol. 9: 114.