hCG antibody | INN-hFSH-132
No associated image currently available
Mouse anti hCG (Alpha 4 Epitope)
- Product Type
- Monoclonal Antibody
- Clone
- INN-hFSH-132
- Isotype
- IgG1
- Specificity
- hCG
- Region
- (ALPHA 4 EPITOPE)
Antibody Quality and Performance Guarantee
| Mouse anti hCG (Alpha 4 Epitope) antibody, clone INN-hFSH-132 is a high affinity antibody recognising the alpha 4 epitope of the alpha-subunit of Human Chorionic Gonadotrophin (hCG), hLH, hFSH, hTSH and free alpha-subunit. The recognised epitope comprises amino-acids alpha 15 to alpha 22 of the human sequence. Mouse anti hCG (Alpha 4 Epitope) antibody, clone INN-hFSH-132 will not cross-react with glycoprotein hormones of various animal species. |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from ascites
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Immunogen
- Human Follicle Stimulating Hormone (FSH).
- Approx. Protein Concentrations
- IgG concentration 1 mg/ml
- Fusion Partners
- Spleen cells from immunised mice were fused with cells of the X63-Ag8.653 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
1ng/ml | 30ng/ml |
| Immunohistology - Paraffin 1 | |
||
| Immunoprecipitation | |
||
| Western Blotting | |
- 1 This product requires antigen retrieval using steam heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
References for hCG antibody
-
Berger, P. et al. (1988) Antigenic features of human follicle stimulating hormone delineated by monoclonal antibodies and construction of an immunoradiomometric assay.
Endocrinology. 123 (5): 2351-9. -
Berger, P. et al. (1990) Monoclonal antibodies against the free subunits of human chorionic gonadotrophin.
J Endocrinol. 125 (2): 301-9. -
Dirnhofer, S. et al. (1994) Free alpha subunit of human chorionic gonadotrophin: molecular basis of immunologically and biologically active domains.
J Endocrinol. 140 (1): 145-54. -
Zimmermann, G. et al. (2009) Epithelial human chorionic gonadotropin is expressed and produced in human secretory endometrium during the normal menstrual cycle.
Biol Reprod. 80:1053-65. -
Untergasser, G. et al. (2001) Seminal plasma factors induce in vitro PRL secretion in smooth muscle cells of the human prostate.
J Clin Endocrinol Metab. 86: 5577-84. -
Zenzmaier, C. et al. (2011) Decreased levels of genuine large free hCG alpha in men presenting with abnormal semen analysis.
Reprod Biol Endocrinol. 9: 114.
- RRID
- AB_321599
- UniProt
- P01215
- Entrez Gene
- CGA
- GO Terms
- GO:0005576 extracellular region
- GO:0007165 signal transduction
- GO:0005179 hormone activity
- GO:0005625 soluble fraction
- GO:0016486 peptide hormone processing
- GO:0042446 hormone biosynthetic process
MCA1026
155133
161123
L1803
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
View more products with HCG specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
View all Anti-Human ProductsAlways be the first to know.
When we launch new products and resources to help you achieve more in the lab.
Yes, sign me up