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hCG antibody | INN-hCG-72

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Mouse anti hCG (Alpha 6 Epitope)

Product Type
Monoclonal Antibody
Clone
INN-hCG-72
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA493
Datasheet
E R WB
MSDS
0.5 mg loader

Mouse anti Human hCG anibody, clone INN-hCG-72 recognises exclusively free hCG alpha. It reacts with the alpha 6 epitope on hCG alpha. No cross reaction is observed with holo-hCG in direct binding RIA of ELISA.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by ion exchange chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
Human CG.
Approx. Protein Concentrations
Isotype concentration 4.0 mg/ml
Fusion Partners
Spleen cells from immunised mice were fused with cells of the X63-Ag8.653 mouse myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
18 months from date of despatch.

More Information

UniProt
P01215 Related reagents
Entrez Gene
CGA Related reagents
GO Terms
GO:0005576 extracellular region
GO:0007165 signal transduction
GO:0005179 hormone activity
GO:0005625 soluble fraction
GO:0016486 peptide hormone processing
GO:0042446 hormone biosynthetic process
Regulatory
For research purposes only

Applications of hCG antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Radioimmunoassays
Western Blotting
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Product Specific References

References for hCG antibody

  1. Berger, P. et al. (1990) Monoclonal antibodies against the free subunits of human chorionic gonadotrophin.
    J Endocrinol. 125 (2): 301-9.
  2. Madersbacher, S. et al. (1992) Free alpha-subunit, free beta-subunit of human chorionic gonadotropin (hCG), and intact hCG in sera of healthy individuals and testicular cancer patients.
    Clin Chem. 38 (3): 370-6.
  3. Berger, P. et al. (1993) Variants of human chorionic gonadotropin from pregnant women and tumor patients recognized by monoclonal antibodies.
    J Clin Endocrinol Metab. 77 (2): 347-51.
  4. Madersbacher, S. et al. (1993) Serum glycoprotein hormones and their free alpha-subunit in a healthy elderly population selected according to the SENIEUR protocol. Analyses with ultrasensitive time resolved fluoroimmunoassays.
    Mech Ageing Dev. 71 (3): 223-33.
  5. Dirnhofer, S. et al. (1994) Free alpha subunit of human chorionic gonadotrophin: molecular basis of immunologically and biologically active domains.
    J Endocrinol. 140 (1): 145-54.
  6. Hiro'oka, T. et al. (2000) Disulfide bond mutations in follicle-stimulating hormone result in uncoupling of biological activity from intracellular behavior.
    Endocrinology. 141 (12): 4751-6.
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