hCG antibody | INN-hCG-72
Mouse anti Human hCG anibody, clone INN-hCG-72 recognises exclusively free hCG alpha. It reacts with the alpha 6 epitope on hCG alpha. No cross reaction is observed with holo-hCG in direct binding RIA of ELISA.
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Immunogen
- Human CG.
- Approx. Protein Concentrations
- Isotype concentration 4.0 mg/ml
- Fusion Partners
- Spleen cells from immunised mice were fused with cells of the X63-Ag8.653 mouse myeloma cell line.
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P01215
- Entrez Gene
- CGA
- GO Terms
- GO:0005576 extracellular region
- GO:0007165 signal transduction
- GO:0005179 hormone activity
- GO:0005625 soluble fraction
- GO:0016486 peptide hormone processing
- GO:0042446 hormone biosynthetic process
- Regulatory
- For research purposes only
Applications of hCG antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | |||
| Radioimmunoassays | |||
| Western Blotting |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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Secondary Antibodies Available
Product Specific References
References for hCG antibody
-
Berger, P. et al. (1990) Monoclonal antibodies against the free subunits of human chorionic gonadotrophin.
J Endocrinol. 125 (2): 301-9. -
Madersbacher, S. et al. (1992) Free alpha-subunit, free beta-subunit of human chorionic gonadotropin (hCG), and intact hCG in sera of healthy individuals and testicular cancer patients.
Clin Chem. 38 (3): 370-6. -
Berger, P. et al. (1993) Variants of human chorionic gonadotropin from pregnant women and tumor patients recognized by monoclonal antibodies.
J Clin Endocrinol Metab. 77 (2): 347-51. -
Madersbacher, S. et al. (1993) Serum glycoprotein hormones and their free alpha-subunit in a healthy elderly population selected according to the SENIEUR protocol. Analyses with ultrasensitive time resolved fluoroimmunoassays.
Mech Ageing Dev. 71 (3): 223-33. -
Dirnhofer, S. et al. (1994) Free alpha subunit of human chorionic gonadotrophin: molecular basis of immunologically and biologically active domains.
J Endocrinol. 140 (1): 145-54. -
Hiro'oka, T. et al. (2000) Disulfide bond mutations in follicle-stimulating hormone result in uncoupling of biological activity from intracellular behavior.
Endocrinology. 141 (12): 4751-6.
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