Mouse anti Human hCG anibody, clone INN-hCG-72 recognises exclusively free hCG alpha. It reacts with the alpha 6 epitope on hCG alpha. No cross reaction is observed with holo-hCG in direct binding RIA of ELISA.
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- Human CG.
- Approx. Protein Concentrations
- Isotype concentration 4.0 mg/ml
- Fusion Partners
- Spleen cells from immunised mice were fused with cells of the X63-Ag8.653 mouse myeloma cell line.
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
peptide hormone processing
hormone biosynthetic process
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of hCG antibody
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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Secondary Antibodies Available
Product Specific References
References for hCG antibody
Berger, P. et al. (1990) Monoclonal antibodies against the free subunits of human chorionic gonadotrophin.
J Endocrinol. 125 (2): 301-9.
Madersbacher, S. et al. (1992) Free alpha-subunit, free beta-subunit of human chorionic gonadotropin (hCG), and intact hCG in sera of healthy individuals and testicular cancer patients.
Clin Chem. 38 (3): 370-6.
Berger, P. et al. (1993) Variants of human chorionic gonadotropin from pregnant women and tumor patients recognized by monoclonal antibodies.
J Clin Endocrinol Metab. 77 (2): 347-51.
Madersbacher, S. et al. (1993) Serum glycoprotein hormones and their free alpha-subunit in a healthy elderly population selected according to the SENIEUR protocol. Analyses with ultrasensitive time resolved fluoroimmunoassays.
Mech Ageing Dev. 71 (3): 223-33.
Dirnhofer, S. et al. (1994) Free alpha subunit of human chorionic gonadotrophin: molecular basis of immunologically and biologically active domains.
J Endocrinol. 140 (1): 145-54.
Hiro'oka, T. et al. (2000) Disulfide bond mutations in follicle-stimulating hormone result in uncoupling of biological activity from intracellular behavior.
Endocrinology. 141 (12): 4751-6.
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