hCG antibody | INN-hCG-72
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Mouse anti Human hCG anibody, clone INN-hCG-72 recognises exclusively free hCG alpha. It reacts with the alpha 6 epitope on hCG alpha. No cross reaction is observed with holo-hCG in direct binding RIA of ELISA.
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by ion exchange chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Immunogen
- Human CG.
- Approx. Protein Concentrations
- Isotype concentration 4.0 mg/ml
- Fusion Partners
- Spleen cells from immunised mice were fused with cells of the X63-Ag8.653 mouse myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 18 months from date of despatch.
More Information
- UniProt
- P01215 Related reagents
- Entrez Gene
- CGA Related reagents
- GO Terms
- GO:0005576 extracellular region
- GO:0007165 signal transduction
- GO:0005179 hormone activity
- GO:0005625 soluble fraction
- GO:0016486 peptide hormone processing
- GO:0042446 hormone biosynthetic process
- Regulatory
- For research purposes only
Applications of hCG antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | |||
| Radioimmunoassays | |||
| Western Blotting |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Product Specific References
References for hCG antibody
-
Berger, P. et al. (1990) Monoclonal antibodies against the free subunits of human chorionic gonadotrophin.
J Endocrinol. 125 (2): 301-9. -
Madersbacher, S. et al. (1992) Free alpha-subunit, free beta-subunit of human chorionic gonadotropin (hCG), and intact hCG in sera of healthy individuals and testicular cancer patients.
Clin Chem. 38 (3): 370-6. -
Berger, P. et al. (1993) Variants of human chorionic gonadotropin from pregnant women and tumor patients recognized by monoclonal antibodies.
J Clin Endocrinol Metab. 77 (2): 347-51. -
Madersbacher, S. et al. (1993) Serum glycoprotein hormones and their free alpha-subunit in a healthy elderly population selected according to the SENIEUR protocol. Analyses with ultrasensitive time resolved fluoroimmunoassays.
Mech Ageing Dev. 71 (3): 223-33. -
Dirnhofer, S. et al. (1994) Free alpha subunit of human chorionic gonadotrophin: molecular basis of immunologically and biologically active domains.
J Endocrinol. 140 (1): 145-54. -
Hiro'oka, T. et al. (2000) Disulfide bond mutations in follicle-stimulating hormone result in uncoupling of biological activity from intracellular behavior.
Endocrinology. 141 (12): 4751-6.
