hCG antibody | INN-hCG-53
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Mouse anti hCG Holo
- Product Type
- Monoclonal Antibody
- Clone
- INN-hCG-53
- Isotype
- IgG1
- Specificity
- hCG
- Region
- HOLO
Antibody Quality and Performance Guarantee
| Mouse anti holo-chorilonic gonadotrophin anribody, clone INN-hCG-53 recognises epitope c2 on hCG. It does not recognize free hCG subunits. |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
||
| Western Blotting | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- Clone INN-hCG-53 detects a band of about 47-50 kDa under non-reducing conditions.
References for hCG antibody
-
Lei, Z.M. et al. (1993) Novel coexpression of human chorionic gonadotropin (hCG)/human luteinizing hormone receptors and their ligand hCG in human fallopian tubes.
J Clin Endocrinol Metab. 77 (3): 863-72. -
Jackson, A.M. et al. (1999) The biological action of choriogonadotropin is not dependent on the complete native quaternary interactions between the subunits.
Mol Endocrinol. 13 (12): 2175-88. -
Klonisch, T. et al. (1996) Relative location of epitopes involved in synergistic antibody binding using human chorionic gonadotropin as a model.
Eur J Immunol. 26: 1897-905.
Further Reading
-
Ben-Menahem, D. et al. (2001) The position of the alpha and beta subunits in a single chain variant of human chorionic gonadotropin affects the heterodimeric interaction of the subunits and receptor-binding epitopes.
J Biol Chem. 276 (32): 29871-9.
- RRID
- AB_322005
- UniProt
- P01233
- P01215
- Entrez Gene
- CGA
- CGB
- GO Terms
- GO:0005576 extracellular region
- GO:0007165 signal transduction
- GO:0005179 hormone activity
- GO:0005625 soluble fraction
- GO:0016486 peptide hormone processing
- GO:0042446 hormone biosynthetic process
MCA1436
148130
156106
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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