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hCG antibody | INN-hCG-2

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Mouse anti hCG (Beta 1 Epitope)

Product Type
Monoclonal Antibody
Clone
INN-hCG-2
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA19
Datasheet
E IY P R
SDS
0.5 mg loader

Mouse anti hCG antibody, clone INN-hCG-2 recognizes the beta 1 epitope on human chorionic gonadotrophin (hCG).

Chorionic gonadotrophin is a member of the glycoprotein hormone family that is synthesized by the placenta throughout pregnancy. The glycoprotein hormone family has 4 members, the other three being luteinising hormone (LH), follicle stimulating hormone (FSH) and thyroid stimulating hormone (TSH). All consist of two subunits, alpha and beta. The alpha subunit is highly homologous between family members.

Mouse anti hCG antibody, clone INN-hCG-2 is reactive with hCG, hCGbeta, hCGbetacf (fragment), hCGn (nicked), hCGbetan, and CTP-hCG. No cross reaction is observed with hLH, hLHbeta, hFSH, hTSH, or the glycoprotein hormone alpha chains.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
hCG.
Approx. Protein Concentrations
Ig concentration 1.0 mg/ml
Fusion Partners
Spleen cells from an immunised BALB/c mouse were fused with the mouse P3-NS1/1 - Ag 4.1 myeloma cell line.

Storage Information

Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P01233
Entrez Gene
CGB
Regulatory
For research purposes only

Applications of hCG antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Flow Cytometry
Immunoassay
Immunohistology - Paraffin
Radioimmunoassays
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

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Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Product Specific References

References for hCG antibody

  1. Kofler, R. et al. (1982) Monoclonal antibodies against human chorionic gonadotropin (hCG): I. production, specificity, and intramolecular binding sites.
    Am J Reprod Immunol. 2 (4): 212-6.
  2. Berger, P. et al. (1993) Variants of human chorionic gonadotropin from pregnant women and tumor patients recognized by monoclonal antibodies.
    J Clin Endocrinol Metab. 77 (2): 347-51.
  3. Zimmermann, G. et al. (2009) Zimmermann G, Ackermann W, Alexander H. Epithelial human chorionic Epithelial human chorionic gonadotropin is expressed and produced in human secretory endometrium during the normal menstrual cycle. Biol Reprod. 2009 May;80(5):1053-65. Epub 2009 Jan 21. PubMed PMID: 19164178.
    Biol Reprod. 80:1053-65.
  4. Zimmermann, G. et al. (2003) Expression of beta hCG and alpha CG mRNA and hCG hormone in human decidual tissue in patients during tubal pregnancy.
    Mol Hum Reprod. 9: 81-9.
  5. Charrel-Dennis, M. et al. (2005) The human chorionic gonadotropin-beta arginine68 to glutamic acid substitution fixes the conformation of the C-terminal peptide.
    Mol Endocrinol. 19: 1803-11.
  6. Schwalenberg, T. et al. (2012) Enhanced urothelial expression of human chorionic gonadotropin beta (hCGβ) in bladder pain syndrome/interstitial cystitis (BPS/IC).
    World J Urol. 30 (3): 411-7.

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