H-Ras antibody | H-Ras-03

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Mouse anti Human H-Ras

Product Type
Monoclonal Antibody
Product Code Applications Pack Size List Price Quantity
0.1 mg loader

Mouse anti Human H-Ras antibody, clone H-Ras-03 recognizes the human GTPase HRas also known as H-Ras, Transforming protein p21 or p21ras. H-Ras is a ubiquitously expressed lipid-anchored GTPase belonging to the Ras superfamily of small GTPases, which acts as a molecular signal transduction on/off switch on the inner surface of the plasma membrane and endomembranes (Milburn et al. 1990).

H-Ras, N-Ras and K-Ras are highly related RAS genes which regulate pathways involved in cell differentiation, proliferation, adhesion, migration and apoptosis and are known proto-oncogenes. H-Ras is located within the peripheral plasma membrane and within endomembrane compartments including the ER and Golgi apparatus. In the ER H-Ras can be activated by the RasGRF family exchange factors RasGRF1 and RasGRF2, this process does not appear to happen withing the Golgi (Arozarena et al. 2004). Mutations in the H-Ras gene are responsible for the rare condition known as Costello syndrome characterized by facial, cardiovascular and musculoskeletal abnormalities and mental retardation (Gripp et al. 2006).

Mouse anti Human H-Ras, clone H-Ras-03 has been used for the detection of H-Ras in the MCF10A human epitheliod breast cancer cell line by Western blotting (Vuoriluoto et al. 2011).

Product Details

Target Species
Species Cross-Reactivity
Target SpeciesCross Reactivity
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Synthetic peptide corresponding to amino acids DIHQYREQIKRVKDSDDC of human H-Ras.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
12 months from date of despatch

More Information

Entrez Gene
GO Terms
GO:0005886 plasma membrane
GO:0000139 Golgi membrane
GO:0000186 activation of MAPKK activity
GO:0003924 GTPase activity
GO:0007596 blood coagulation
GO:0005829 cytosol
GO:0005525 GTP binding
GO:0007050 cell cycle arrest
GO:0007173 epidermal growth factor receptor signaling pathway
GO:0007265 Ras protein signal transduction
GO:0007268 synaptic transmission
GO:0009887 organ morphogenesis
GO:0007411 axon guidance
GO:0008022 protein C-terminus binding
GO:0008285 negative regulation of cell proliferation
GO:0008286 insulin receptor signaling pathway
GO:0031575 mitotic cell cycle G1/S transition checkpoint
GO:0045740 positive regulation of DNA replication
GO:0048011 nerve growth factor receptor signaling pathway
GO:0050679 positive regulation of epithelial cell proliferation
GO:0050900 leukocyte migration
GO:0090398 cellular senescence
For research purposes only

Applications of H-Ras antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 0.5 1.0ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
MCA2884 detects a band of approximately 24kDa in HeLa, K567 and RAJI whole cell lysates under reducing conditions.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Product Specific References

References for H-Ras antibody

  1. Vuoriluoto, K. et al. (2011) Vimentin regulates EMT induction by Slug and oncogenic H-Ras and migration by governing Axl expression in breast cancer.
    Oncogene. 30: 1436-48.

Further Reading

  1. Zheng, Y. & Quilliam, L.A. (2003) Activation of the Ras superfamily of small GTPases. Workshop on exchange factors.
    EMBO Rep. 4 (5): 463-8.

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