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H-Ras antibody | H-Ras-03

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Anti Human H-Ras Antibody, clone H-Ras-03 gallery image 1
Western blot analysis of HEK293 embryonic kidney cell line whole cell lysate probed with Mouse anti Human H-Ras antibody (MCA2884) followed by HRP conjugated Goat anti Mouse IgG secondary antibody visualized using chemiluminescence.

Mouse anti Human H-Ras

Product Type
Monoclonal Antibody
Clone
H-Ras-03
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA2884
Datasheet
E WB
SDS
0.1 mg loader

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P01112
Entrez Gene
HRAS
GO Terms
GO:0005886 plasma membrane
GO:0000139 Golgi membrane
GO:0000186 activation of MAPKK activity
GO:0003924 GTPase activity
GO:0007596 blood coagulation
GO:0005829 cytosol
GO:0005525 GTP binding
GO:0007050 cell cycle arrest
GO:0007173 epidermal growth factor receptor signaling pathway
GO:0007265 Ras protein signal transduction
GO:0007268 synaptic transmission
GO:0009887 organ morphogenesis
GO:0007411 axon guidance
GO:0008022 protein C-terminus binding
GO:0008285 negative regulation of cell proliferation
GO:0008286 insulin receptor signaling pathway
GO:0031575 mitotic cell cycle G1/S transition checkpoint
GO:0045740 positive regulation of DNA replication
GO:0048011 nerve growth factor receptor signaling pathway
GO:0050679 positive regulation of epithelial cell proliferation
GO:0050900 leukocyte migration
GO:0090398 cellular senescence
Regulatory
For research purposes only

Applications of H-Ras antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Western Blotting 0.5 1.0ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
MCA2884 detects a band of approximately 24kDa in HeLa, K567 and RAJI whole cell lysates under reducing conditions.
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Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Product Specific References

References for H-Ras antibody

  1. Vuoriluoto, K. et al. (2011) Vimentin regulates EMT induction by Slug and oncogenic H-Ras and migration by governing Axl expression in breast cancer.
    Oncogene. 30: 1436-48.

Further Reading

  1. Zheng, Y. & Quilliam, L.A. (2003) Activation of the Ras superfamily of small GTPases. Workshop on exchange factors.
    EMBO Rep. 4 (5): 463-8.

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