H-Ras antibody | H-Ras-03
siRNA Validated
Filter by Application:
WB ResetMouse anti Human H-Ras
- Product Type
- Monoclonal Antibody
- Clone
- H-Ras-03
- Isotype
- IgG1
- Format
- Purified
- Specificity
- H-Ras
Antibody Quality and Performance Guarantee
| Mouse anti Human H-Ras antibody, clone H-Ras-03 recognizes the human GTPase HRas also known as H-Ras, Transforming protein p21 or p21ras. H-Ras is a ubiquitously expressed lipid-anchored GTPase belonging to the Ras superfamily of small GTPases, which acts as a molecular signal transduction on/off switch on the inner surface of the plasma membrane and endomembranes ( H-Ras, N-Ras and K-Ras are highly related RAS genes which regulate pathways involved in cell differentiation, proliferation, adhesion, migration and apoptosis and are known proto-oncogenes. H-Ras is located within the peripheral plasma membrane and within endomembrane compartments including the ER and Golgi apparatus. In the ER H-Ras can be activated by the RasGRF family exchange factors RasGRF1 and RasGRF2, this process does not appear to happen withing the Golgi (Arozarena et al. 2004). Mutations in the H-Ras gene are responsible for the rare condition known as Costello syndrome characterized by facial, cardiovascular and musculoskeletal abnormalities and mental retardation (Gripp et al. 2006). Mouse anti Human H-Ras, clone H-Ras-03 has been used for the detection of H-Ras in the MCF10A human epitheliod breast cancer cell line by Western blotting (Vuoriluoto et al. 2011). |
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- Target Species
- Human
- Western Blotting
- MCA2884 detects a band of approximately 24kDa in HeLa, K567 and RAJI whole cell lysates under reducing conditions.
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from ascites
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- Synthetic peptide corresponding to amino acids DIHQYREQIKRVKDSDDC of human H-Ras.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
||
| Western Blotting | |
0.5 | 1.0ug/ml |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
References for H-Ras antibody
-
Vuoriluoto, K. et al. (2011) Vimentin regulates EMT induction by Slug and oncogenic H-Ras and migration by governing Axl expression in breast cancer.
Oncogene. 30: 1436-48.
Further Reading
-
Zheng, Y. & Quilliam, L.A. (2003) Activation of the Ras superfamily of small GTPases. Workshop on exchange factors.
EMBO Rep. 4 (5): 463-8.
- Synonyms
- p21Ras
- RRID
- AB_2121038
- UniProt
- P01112
- Entrez Gene
- HRAS
- GO Terms
- GO:0005886 plasma membrane
- GO:0000139 Golgi membrane
- GO:0000186 activation of MAPKK activity
- GO:0003924 GTPase activity
- GO:0007596 blood coagulation
- GO:0005829 cytosol
- GO:0005525 GTP binding
- GO:0007050 cell cycle arrest
- GO:0007173 epidermal growth factor receptor signaling pathway
- View More GO Terms
- GO:0007265 Ras protein signal transduction
- GO:0007268 synaptic transmission
- GO:0009887 organ morphogenesis
- GO:0007411 axon guidance
- GO:0008022 protein C-terminus binding
- GO:0008285 negative regulation of cell proliferation
- GO:0008286 insulin receptor signaling pathway
- GO:0031575 mitotic cell cycle G1/S transition checkpoint
- GO:0045740 positive regulation of DNA replication
- GO:0048011 nerve growth factor receptor signaling pathway
- GO:0050679 positive regulation of epithelial cell proliferation
- GO:0050900 leukocyte migration
- GO:0090398 cellular senescence
MCA2884
148819
165599
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