Granzyme B antibody | GB7

Mouse anti Human Granzyme B

Product Type
Monoclonal Antibody
Clone
GB7
Isotype
IgG2a
Specificity
Granzyme B

Product Code Applications Pack Size List Price Your Price Qty
MCA2118
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
P * 0.1 mg loader
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loader
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Mouse anti Human Granzyme B antibody, clone GB7 recognizes the serine protease Granzyme B, expressed within the granules of cytolytic lymphocytes (CTLs)

Granzyme B plays a key role in the induction of apoptosis by CTLs. After delivery to the target cell Granzyme B activates the cascade of caspases that finally results in cell death.

Mouse anti Human Granzyme B antibody, clone GB7 has been reported to be suitable for Western Blotting, demonstrating reactivity with recombinant granzyme B and also reactivity with recombinant granzyme H (Sedelies et al. 2004). Human granzymes B and H share ~71% sequence identity by clustal analysis.

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.01% Thiomersal
Immunogen
Recombinant Granzyme B.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1 1/50 1/200
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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References for Granzyme B antibody

  1. (2011) Human Protein Atlas: CAB000376
    Human Protein Atlas: CAB000376
  2. Kummer, J.A. et al. (1993) Production and characterization of monoclonal antibodies raised against recombinant human granzymes A and B and showing cross reactions with the natural proteins.
    J Immunol Methods. 163 (1): 77-83.
  3. Nield, L.E. et al. (2002) Endocardial fibroelastosis associated with maternal anti-Ro and anti-La antibodies in the absence of atrioventricular block.
    J Am Coll Cardiol. 40: 796-802.
  4. Himmelein, S. et al. (2015) Latent herpes simplex virus 1 infection does not induce apoptosis in human trigeminal Ganglia.
    J Virol. 89 (10): 5747-50.
  5. Cohen, M. et al. (2018) Epstein-Barr virus lytic cycle involvement in diffuse large B cell lymphoma.
    Hematol Oncol. 36 (1): 98-103.
  6. Sedelies, K.A. et al. (2004) Discordant regulation of granzyme H and granzyme B expression in human lymphocytes.
    J Biol Chem. 279 (25): 26581-7.

Immunohistology - Paraffin

Western Blotting

RRID
AB_323716
UniProt
P10144
Entrez Gene
GZMB
GO Terms
GO:0005515 protein binding
GO:0001772 immunological synapse
GO:0004252 serine-type endopeptidase activity
GO:0005829 cytosol
GO:0005634 nucleus
GO:0006922 cleavage of lamin
GO:0019835 cytolysis
GO:0008629 induction of apoptosis by intracellular signals
GO:0008633 activation of pro-apoptotic gene products
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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