Granzyme B antibody | GB7

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Mouse anti Human Granzyme B

Product Type
Monoclonal Antibody
Clone
GB7
Isotype
IgG2a
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2118T P* 20 µg
MCA2118 P* WB 0.1 mg
Mouse anti Human Granzyme B antibody, clone GB7 recognizes the serine protease Granzyme B, which is expressed within the granules of cytolytic lymphocytes (CTLs)

Granzyme B plays a key role in the induction of apoptosis by CTLs. After delivery to the target cell Granzyme B activates the cascade of caspases that finally results in cell death.

Mouse anti Human Granzyme B antibody, clone GB7 has been reported to be suitable for Western Blotting.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.01%Thiomersal
Immunogen
Recombinant Granzyme B.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P10144 Related reagents
Entrez Gene
GZMB Related reagents
GO Terms
GO:0005515 protein binding
GO:0001772 immunological synapse
GO:0004252 serine-type endopeptidase activity
GO:0005829 cytosol
GO:0005634 nucleus
GO:0006922 cleavage of lamin
GO:0019835 cytolysis
GO:0008629 induction of apoptosis by intracellular signals
GO:0008633 activation of pro-apoptotic gene products
Regulatory
For research purposes only

Applications of Granzyme B antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1 1/50 1/200
Western Blotting
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG2a:FITC HCA037F 0.1 mg F
Human anti Mouse IgG2a:HRP HCA037P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Application Based External Images

Immunohistology - Paraffin

Western Blotting

Product Specific References

References for Granzyme B antibody

  1. Kummer, J.A. et al. (1993) Production and characterization of monoclonal antibodies raised against recombinant human granzymes A and B and showing cross reactions with the natural proteins.
    J Immunol Methods. 163 (1): 77-83.
  2. Nield, L.E. et al. (2002) Endocardial fibroelastosis associated with maternal anti-Ro and anti-La antibodies in the absence of atrioventricular block.
    J Am Coll Cardiol. 40: 796-802.
  3. Himmelein, S. et al. (2015) Latent herpes simplex virus 1 infection does not induce apoptosis in human trigeminal Ganglia.
    J Virol. 89 (10): 5747-50.
  4. Cohen, M. et al. (2017) Epstein-Barr virus lytic cycle involvement in diffuse large B cell lymphoma.
    Hematol Oncol. Jul 14 [Epub ahead of print].
  5. Sedelies, K.A. et al. (2004) Discordant regulation of granzyme H and granzyme B expression in human lymphocytes.
    J Biol Chem. 279 (25): 26581-7.