FSH antibody | F1 (BGN/F62/01)

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Mouse anti Human FSH Alpha:Biotin

Mouse anti Human FSH Alpha

Product Type
Monoclonal Antibody
Clone
F1 (BGN/F62/01)
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
0100-0662B E 0.5 mg
0100-0662 E P 1 mg
Mouse anti Human FSH alpha antibody, clone F1 (BGN/F62/01) detects the alpha subunit of Follicle-stimulating hormone (FSH alpha). FSH is secreted by the pituitary, and is a member of the glycoprotein hormone family which includes Human chorionic gonadotropin (hCG), Luteinizing hormone (LH), and Thyroid stimulating hormone (TSH).

These hormones are all structurally related and contain a common alpha subunit non-covalently bound to a hormone specific beta subunit, which determines receptor specificity. Both of the subunits are necessary for hormone action.

Product Details

Target Species
Human
Product Form
Purified IgG conjugated to Biotin - liquid
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Immunogen
Native human follicle stimulating hormone.
Approx. Protein Concentrations
1.0mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P01215 Related reagents
Entrez Gene
CGA Related reagents
GO Terms
GO:0005576 extracellular region
GO:0007165 signal transduction
GO:0005179 hormone activity
GO:0005625 soluble fraction
GO:0016486 peptide hormone processing
GO:0042446 hormone biosynthetic process
Regulatory
For research purposes only

Applications of FSH antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/100 1/1000
ELISA
Immunohistology - Paraffin
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
ELISA
0100-0662B is suitable for use in a two site assay with 0100-0663 bound to the solid phase and this antibody for detection. When tested in a sandwich ELISA the 50% OD was observed at an antibody dilution of 1/160K and the end point titre at 1/5M.
ELISA
0100-0662 is suitable for use as a detection antibody in a sandwich ELISA assay with 0100-0663 as the capture antibody.

Application Based External Images

Immunohistology - Paraffin

Product Specific References

References for FSH antibody

  1. Dreessen, I.L.E. (2017) Development of a dog-specific Enzyme-Linked Immuno Sorbent Assay (ELISA) for detecting Luteinising Hormone (LH) and Follicle Stimulating Hormone (FSH) in plasma
    Faculty of Veterinary Medicine Theses Utrecht University Repository

Further Reading

  1. Sohn, J. et al. (2003) Orientation of follicle-stimulating hormone (FSH) subunits complexed with the FSH receptor. Beta subunit toward the N terminus of exodomain and alpha subunit to exoloop 3.
    J Biol Chem. 278 (48): 47868-76.