FOXP3 antibody | rA16B16-01/2F5
Mouse anti Human FOXP3
- Product Type
- Monoclonal Antibody
- Clone
- rA16B16-01/2F5
- Isotype
- IgG1
- Specificity
- FOXP3
Antibody Quality and Performance Guarantee
TZA083GA
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| Mouse anti Human FoxP3 antibody, clone rA16B16-01/2F5, is a recombinant monoclonal antibody that recognizes FoxP3, also known as Scurfin or IPEX. FoxP3 is a member of the forkhead transcription factor family. It is believed to be a master regulatory gene and is crucial for the development and inhibitory function of regulatory T-cells (Tregs). FoxP3 plays a central role in immune system homeostasis by driving the suppressive function and stability of the Treg lineage, through which it directly modulates the function and expansion of all T cells. The suppressive activity of Tregs involves the coordinated activation of several genes by FoxP3, including CTLA-4 and TNFRSF1, along with the repression of genes encoding inflammatory cytokines, such as interleukin-2 (IL-2) and interferon-gamma (IFNγ). FoxP3 is downregulated during inflammation to allow for the acquisition of effector T cell-like functions. FoxP3 inhibits cytokine production and T-cell effector function by repressing the activity of two key transcription factors, RELA and NFATC2. FoxP3 is a key marker for CD4+ Tregs. FoxP3-expressing CD4+ Tregs have a key role in immune tolerance and suppression of autoimmune and anti tumor immune responses. FoxP3 deficiency causes immune dysregulation and enteropathy X-linked syndrome (IPEX). |
- Target Species
- Human
- Product Form
- Purified recombinant IgG containing a SpyTag at the C-terminus of the antibody heavy chain – liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Source
- CHO cells
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Recombinant FoxP3 Met1-Arg417 expressed by E.coli
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 5μl of the suggested working dilution to label 0.5x106 cells in 100μl. Best practices suggest a 5 min centrifugation at 6,000g prior to sample application.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control | ||||||
- Synonyms
- Scurfin
- UniProt
- Q9BZS1
- Entrez Gene
- FOXP3
- GO Terms
- GO:0001782 B cell homeostasis
- GO:0002053 positive regulation of mesenchymal cell proliferation
- GO:0002262 myeloid cell homeostasis
- GO:0002513 tolerance induction to self antigen
- GO:0002669 positive regulation of T cell anergy
- GO:0002677 negative regulation of chronic inflammatory response
- GO:0002725 negative regulation of T cell cytokine production
- GO:0002851 positive regulation of peripheral T cell tolerance induction
- GO:0003682 chromatin binding
- View More GO Terms
- GO:0003690 double-stranded DNA binding
- GO:0003704 specific RNA polymerase II transcription factor activity
- GO:0003705 sequence-specific enhancer binding RNA polymerase II transcription factor activity
- GO:0003714 transcription corepressor activity
- GO:0005634 nucleus
- GO:0005737 cytoplasm
- GO:0005667 transcription factor complex
- GO:0006338 chromatin remodeling
- GO:0040007 growth
- GO:0009790 embryo development
- GO:0007389 pattern specification process
- GO:0007519 skeletal muscle tissue development
- GO:0008270 zinc ion binding
- GO:0008301 DNA bending activity
- GO:0009791 post-embryonic development
- GO:0010552 positive regulation of gene-specific transcription from RNA polymerase II promoter
- GO:0010553 negative regulation of gene-specific transcription from RNA polymerase II promoter
- GO:0010843 promoter binding
- GO:0016563 transcription activator activity
- GO:0016566 specific transcriptional repressor activity
- GO:0021549 cerebellum development
- GO:0021757 caudate nucleus development
- GO:0021758 putamen development
- GO:0021987 cerebral cortex development
- GO:0031064 negative regulation of histone deacetylation
- GO:0032088 negative regulation of NF-kappaB transcription factor activity
- GO:0032689 negative regulation of interferon-gamma production
- GO:0032693 negative regulation of interleukin-10 production
- GO:0032703 negative regulation of interleukin-2 production
- GO:0032713 negative regulation of interleukin-4 production
- GO:0032714 negative regulation of interleukin-5 production
- GO:0032715 negative regulation of interleukin-6 production
- GO:0032720 negative regulation of tumor necrosis factor production
- GO:0032792 negative regulation of CREB transcription factor activity
- GO:0032831 positive regulation of CD4-positive, CD25-positive, alpha-beta regulatory T cell differentiation
- GO:0032914 positive regulation of transforming growth factor-beta1 production
- GO:0033092 positive regulation of immature T cell proliferation in thymus
- GO:0035035 histone acetyltransferase binding
- GO:0035066 positive regulation of histone acetylation
- GO:0035067 negative regulation of histone acetylation
- GO:0042110 T cell activation
- GO:0042803 protein homodimerization activity
- GO:0042826 histone deacetylase binding
- GO:0043010 camera-type eye development
- GO:0043029 T cell homeostasis
- GO:0045077 negative regulation of interferon-gamma biosynthetic process
- GO:0045085 negative regulation of interleukin-2 biosynthetic process
- GO:0046007 negative regulation of activated T cell proliferation
- GO:0046982 protein heterodimerization activity
- GO:0048286 lung alveolus development
- GO:0048294 negative regulation of isotype switching to IgE isotypes
- GO:0048302 regulation of isotype switching to IgG isotypes
- GO:0048745 smooth muscle tissue development
- GO:0050710 negative regulation of cytokine secretion
- GO:0050852 T cell receptor signaling pathway
- GO:0051059 NF-kappaB binding
- GO:0051525 NFAT protein binding
- GO:0060501 positive regulation of epithelial cell proliferation involved in lung morphogenesis
View more products with FOXP specificity
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