Factor VII antibody | RFF-VII/2

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Mouse anti Human Factor VII

Product Type: Monoclonal Antibody
Clone: RFF-VII/2
Isotype: IgG1

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
MCA4680	E R WB*			0.5 mg
MCA4680G	E R WB*			0.2 mg

Mouse anti Human Factor VII antibody, clone RFF-VII/2 recognizes Factor VII, a serine protease found circulating in the blood. It initiates the extrinsic pathway of blood coagulation in conjunction with tissue factor. Tissue factor is found on the outside of blood vessels. When the vessels is damaged, tissue factor is exposed to the blood and circulating Factor VII. Once bound to tissue factor, Factor VII is activated by different proteases and converts Factor IX to IXa. Factor VII deficiency (FA7D) is a rare hereditary haemorrhagic disease. Symptoms range from mild to severe.

Mouse anti Human Factor VII antibody, clone RFF-VII/2 may be used as a capture antibody in immunoassays for Factor VII in combination with Mouse anti Human Factor VII antibody, clone RFF-VII/1 (MCA4681) as a detection reagent.

Product Details

Target Species: Human
Product Form: Purified IgG - liquid
Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
Carrier Free: Yes
Immunogen: Purified Factor VII.
Approx. Protein Concentrations: IgG concentration 1.0mg/ml
Fusion Partners: Spleen cells from immunised Balb/c mice were fused with cells of the P3-NS/1-Ag 4 mouse myeloma cell line.

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

More Information

UniProt: P08709 Related reagents
Entrez Gene: F7 Related reagents
GO Terms: GO:0005886 plasma membrane; GO:0005509 calcium ion binding; GO:0001948 glycoprotein binding; GO:0002690 positive regulation of leukocyte chemotaxis; GO:0004252 serine-type endopeptidase activity; GO:0005788 endoplasmic reticulum lumen; GO:0005796 Golgi lumen; GO:0006508 proteolysis; GO:0006916 anti-apoptosis; GO:0007598 blood coagulation, extrinsic pathway; GO:0010641 positive regulation of platelet-derived growth factor receptor signaling pathway; GO:0017187 peptidyl-glutamic acid carboxylation; GO:0043687 post-translational protein modification; GO:0050927 positive regulation of positive chemotaxis; GO:0051897 positive regulation of protein kinase B signaling cascade
Regulatory: For research purposes only

Applications of Factor VII antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA
Radioimmunoassays
Western Blotting ¹

¹MCA4680 binds to a conformational epitope and will not bind to reduced Factor VII.

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Product Specific References

References for Factor VII antibody

Takase, T. et al. (1988) Monoclonal antibodies to human factor VII: a one step immunoradiometric assay for VII:Ag.
J Clin Pathol. 41 (3): 337-41.

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