Factor VII antibody | RFF-VII/2

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Mouse anti Human Factor VII

Product Type
Monoclonal Antibody
Clone
RFF-VII/2
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA4680G E R WB* 0.2 mg
MCA4680 E R WB* 0.5 mg
Mouse anti Human Factor VII antibody, clone RFF-VII/2 recognizes Factor VII, a serine protease found circulating in the blood. It initiates the extrinsic pathway of blood coagulation in conjunction with tissue factor. Tissue factor is found on the outside of blood vessels. When the vessels is damaged, tissue factor is exposed to the blood and circulating Factor VII. Once bound to tissue factor, Factor VII is activated by different proteases and converts Factor IX to IXa. Factor VII deficiency (FA7D) is a rare hereditary haemorrhagic disease. Symptoms range from mild to severe.

Mouse anti Human Factor VII antibody, clone RFF-VII/2 may be used as a capture antibody in immunoassays for Factor VII in combination with Mouse anti Human Factor VII antibody, clone RFF-VII/1 (MCA4681) as a detection reagent.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Purified Factor VII.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Fusion Partners
Spleen cells from immunised Balb/c mice were fused with cells of the P3-NS/1-Ag 4 mouse myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P08709 Related reagents
Entrez Gene
F7 Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0005509 calcium ion binding
GO:0001948 glycoprotein binding
GO:0002690 positive regulation of leukocyte chemotaxis
GO:0004252 serine-type endopeptidase activity
GO:0005788 endoplasmic reticulum lumen
GO:0005796 Golgi lumen
GO:0006508 proteolysis
GO:0006916 anti-apoptosis
GO:0007598 blood coagulation, extrinsic pathway
GO:0010641 positive regulation of platelet-derived growth factor receptor signaling pathway
GO:0017187 peptidyl-glutamic acid carboxylation
GO:0043687 post-translational protein modification
GO:0050927 positive regulation of positive chemotaxis
GO:0051897 positive regulation of protein kinase B signaling cascade
Regulatory
For research purposes only

Applications of Factor VII antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Radioimmunoassays
Western Blotting 1
  1. 1MCA4680 binds to a conformational epitope and will not bind to reduced Factor VII.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Product Specific References

References for Factor VII antibody

  1. Takase, T. et al. (1988) Monoclonal antibodies to human factor VII: a one step immunoradiometric assay for VII:Ag.
    J Clin Pathol. 41 (3): 337-41.