Factor VII antibody | RFF-VII/2
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Mouse anti Human Factor VII antibody, clone RFF-VII/2 recognizes Factor VII, a serine protease found circulating in the blood. It initiates the extrinsic pathway of blood coagulation in conjunction with tissue factor. Tissue factor is found on the outside of blood vessels. When the vessels is damaged, tissue factor is exposed to the blood and circulating Factor VII. Once bound to tissue factor, Factor VII is activated by different proteases and converts Factor IX to IXa. Factor VII deficiency (FA7D) is a rare hereditary haemorrhagic disease. Symptoms range from mild to severe.
Mouse anti Human Factor VII antibody, clone RFF-VII/2 may be used as a capture antibody in immunoassays for Factor VII in combination with Mouse anti Human Factor VII antibody, clone RFF-VII/1 (MCA4681) as a detection reagent.
Mouse anti Human Factor VII antibody, clone RFF-VII/2 may be used as a capture antibody in immunoassays for Factor VII in combination with Mouse anti Human Factor VII antibody, clone RFF-VII/1 (MCA4681) as a detection reagent.
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Purified Factor VII.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Fusion Partners
- Spleen cells from immunised Balb/c mice were fused with cells of the P3-NS/1-Ag 4 mouse myeloma cell line.
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P08709
- Entrez Gene
- F7
- GO Terms
- GO:0005886 plasma membrane
- GO:0005509 calcium ion binding
- GO:0001948 glycoprotein binding
- GO:0002690 positive regulation of leukocyte chemotaxis
- GO:0004252 serine-type endopeptidase activity
- GO:0005788 endoplasmic reticulum lumen
- GO:0005796 Golgi lumen
- GO:0006508 proteolysis
- GO:0006916 anti-apoptosis
- GO:0007598 blood coagulation, extrinsic pathway
- GO:0010641 positive regulation of platelet-derived growth factor receptor signaling pathway
- GO:0017187 peptidyl-glutamic acid carboxylation
- GO:0043687 post-translational protein modification
- GO:0050927 positive regulation of positive chemotaxis
- GO:0051897 positive regulation of protein kinase B signaling cascade
- Regulatory
- For research purposes only
Applications of Factor VII antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | |||
| Radioimmunoassays | |||
| Western Blotting 1 |
- 1MCA4680 binds to a conformational epitope and will not bind to reduced Factor VII.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Product Specific References
References for Factor VII antibody
-
Takase, T. et al. (1988) Monoclonal antibodies to human factor VII: a one step immunoradiometric assay for VII:Ag.
J Clin Pathol. 41 (3): 337-41.
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