Elastase antibody | ELA10-101.5 (39A)

Mouse anti Human Elastase

Product Type
Monoclonal Antibody
Clone
ELA10-101.5 (39A)
Isotype
IgG1
Specificity
Elastase

Product Code Applications Pack Size List Price Your Price Qty
4050-0096
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E F FN 0.2 mg loader
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loader
Search for Batch Specific Datasheets

Mouse anti Human elastase antibody, clone 39A recognizes human neutrophil elastase, also known as elastase-2, Bone marrow serine protease, PMN elastase or Medullasin. Elastase is a 238 amino acid ~27 kDa enzyme with an addiional 29 amino acid signal and pro-peptide region. Mutations of the ELA2 gene can lead to a number of hematopoietic disorders including cyclic hematopoiesis (CH) characterized by a 21 day cycle of bone marrow derived blood cell production with neutrophil numbers ranging between normal levels and almost zero (Horwitz et al. 1999) and severe congential autosomal neutropenia (SCN1) demonstating developmental arrest of granulopoiesis at the promyelocyte stage (Bellanné-Chantelot et al. 2004) with both conditions leading to a predisposition to bacterial infections..

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
Elastase from human neutrophils.
Approx. Protein Concentrations
0.5 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Flow Cytometry
Functional Assays
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

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References for Elastase antibody

  1. Durant, S. et al. (2004) Apoptosis-induced proteinase 3 membrane expression is independent from degranulation.
    J Leukoc Biol. 75 (1): 87-98.
  2. Vong, L. et al. (2007) Annexin 1 cleavage in activated neutrophils: a pivotal role for proteinase 3.
    J Biol Chem. 282 (41): 29998-30004.
  3. Davies, P.L. et al. (2008) Monoclonal anti-neutrophil elastase antibody characterisation: ability to block function, detect free versus serpin-complexed enzyme and stain intracellular granules.
    J Immunol Methods. 336 (2): 175-82.
  4. Muthana, M. et al. (2015) C5orf30 is a negative regulator of tissue damage in rheumatoid arthritis.
    Proc Natl Acad Sci U S A. 112 (37): 11618-23.
  5. Buján, J. et al. (2003) Expression of elastic components in healthy and varicose veins.
    World J Surg. 27 (8): 901-5.
  6. Korkmaz, B. et al. (2005) Inhibition of neutrophil elastase by alpha1-protease inhibitor at the surface of human polymorphonuclear neutrophils.
    J Immunol. 175 (5): 3329-38.

ELISA

Synonyms
ELA2
RRID
AB_617258
UniProt
P08246
Entrez Gene
ELANE
GO Terms
GO:0005576 extracellular region
GO:0030141 stored secretory granule
GO:0006508 proteolysis
GO:0006874 cellular calcium ion homeostasis
GO:0008201 heparin binding
GO:0009411 response to UV
GO:0009986 cell surface
GO:0019955 cytokine binding
GO:0030163 protein catabolic process
GO:0043406 positive regulation of MAP kinase activity
GO:0045079 negative regulation of chemokine biosynthetic process
GO:0045415 negative regulation of interleukin-8 biosynthetic process
GO:0045416 positive regulation of interleukin-8 biosynthetic process
GO:0048661 positive regulation of smooth muscle cell proliferation
GO:0050728 negative regulation of inflammatory response
GO:0050922 negative regulation of chemotaxis
GO:0051635 bacterial cell surface binding
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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