Elastase antibody | ELA10-101.5 (39A)

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Mouse anti Human Elastase

Product Type: Monoclonal Antibody
Clone: ELA10-101.5 (39A)
Isotype: IgG1

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
4050-0096	E F FN			0.2 mg

Mouse anti Human elastase antibody, clone 39A recognizes human neutrophil elastase, also known as elastase-2, Bone marrow serine protease, PMN elastase or Medullasin. Elastase is a 238 amino acid ~27 kDa enzyme with an addiional 29 amino acid signal and pro-peptide region. Mutations of the ELA2 gene can lead to a number of hematopoietic disorders including cyclic hematopoiesis (CH) characterized by a 21 day cycle of bone marrow derived blood cell production with neutrophil numbers ranging between normal levels and almost zero (Horwitz et al. 1999) and severe congential autosomal neutropenia (SCN1) demonstating developmental arrest of granulopoiesis at the promyelocyte stage (Bellanné-Chantelot et al. 2004) with both conditions leading to a predisposition to bacterial infections..

Product Details

Target Species

Human

Product Form

Purified IgG - liquid

Preparation

Purified IgG prepared by affinity chromatography on Protein G

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09%

Sodium Azide (NaN₃)

Immunogen

Elastase from human neutrophils.

Approx. Protein Concentrations

0.5 mg/ml

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

More Information

UniProt: P08246 Related reagents
Entrez Gene: ELANE Related reagents
GO Terms: GO:0005576 extracellular region; GO:0030141 stored secretory granule; GO:0006508 proteolysis; GO:0006874 cellular calcium ion homeostasis; GO:0008201 heparin binding; GO:0009411 response to UV; GO:0009986 cell surface; GO:0019955 cytokine binding; GO:0030163 protein catabolic process; GO:0043406 positive regulation of MAP kinase activity; GO:0045079 negative regulation of chemokine biosynthetic process; GO:0045415 negative regulation of interleukin-8 biosynthetic process; GO:0045416 positive regulation of interleukin-8 biosynthetic process; GO:0048661 positive regulation of smooth muscle cell proliferation; GO:0050728 negative regulation of inflammatory response; GO:0050922 negative regulation of chemotaxis; GO:0051635 bacterial cell surface binding
Regulatory: For research purposes only

Applications of Elastase antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA
Flow Cytometry
Functional Assays

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Human anti Mouse IgG1:HRP	HCA036P	0.1 mg	E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Negative Isotype Controls Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Mouse IgG1 Negative Control	MCA928	100 Tests	F

Application Based External Images

ELISA

Product Specific References

References for Elastase antibody

Durant, S. et al. (2004) Apoptosis-induced proteinase 3 membrane expression is independent from degranulation.
J Leukoc Biol. 75 (1): 87-98.
Vong, L. et al. (2007) Annexin 1 cleavage in activated neutrophils: a pivotal role for proteinase 3.
J Biol Chem. 282 (41): 29998-30004.
Davies, P.L. et al. (2008) Monoclonal anti-neutrophil elastase antibody characterisation: ability to block function, detect free versus serpin-complexed enzyme and stain intracellular granules.
J Immunol Methods. 336 (2): 175-82.
Muthana, M. et al. (2015) C5orf30 is a negative regulator of tissue damage in rheumatoid arthritis.
Proc Natl Acad Sci U S A. 112 (37): 11618-23.
Buján, J. et al. (2003) Expression of elastic components in healthy and varicose veins.
World J Surg. 27 (8): 901-5.
Korkmaz, B. et al. (2005) Inhibition of neutrophil elastase by alpha1-protease inhibitor at the surface of human polymorphonuclear neutrophils.
J Immunol. 175 (5): 3329-38.

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