Mouse anti Human elastase antibody, clone 39A
recognizes human neutrophil elastase, also known as elastase-2, Bone marrow serine protease, PMN elastase or Medullasin. Elastase is a 238 amino acid ~27 kDa enzyme with an addiional 29 amino acid signal and pro-peptide region. Mutations of the ELA2 gene can lead to a number of hematopoietic disorders including cyclic hematopoiesis (CH
) characterized by a 21 day cycle of bone marrow derived blood cell production with neutrophil numbers ranging between normal levels and almost zero (Horwitz et al. 1999
) and severe congential autosomal neutropenia (SCN1
) demonstating developmental arrest of granulopoiesis at the promyelocyte stage (Bellanné-Chantelot et al. 2004
) with both conditions leading to a predisposition to bacterial infections..
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein G
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- Elastase from human neutrophils.
- Approx. Protein Concentrations
- 0.5 mg/ml
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 18 months from date of despatch.
- Entrez Gene
- GO Terms
stored secretory granule
cellular calcium ion homeostasis
response to UV
protein catabolic process
positive regulation of MAP kinase activity
negative regulation of chemokine biosynthetic process
negative regulation of interleukin-8 biosynthetic process
positive regulation of interleukin-8 biosynthetic process
positive regulation of smooth muscle cell proliferation
negative regulation of inflammatory response
negative regulation of chemotaxis
bacterial cell surface binding
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of Elastase antibody
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
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Secondary Antibodies Available
Negative Isotype Controls Available
Application Based External Images
Product Specific References
References for Elastase antibody
Durant, S. et al. (2004) Apoptosis-induced proteinase 3 membrane expression is independent from degranulation.
J Leukoc Biol. 75 (1): 87-98.
Vong, L. et al. (2007) Annexin 1 cleavage in activated neutrophils: a pivotal role for proteinase 3.
J Biol Chem. 282 (41): 29998-30004.
Davies, P.L. et al. (2008) Monoclonal anti-neutrophil elastase antibody characterisation: ability to block function, detect free versus serpin-complexed enzyme and stain intracellular granules.
J Immunol Methods. 336 (2): 175-82.
Muthana, M. et al. (2015) C5orf30 is a negative regulator of tissue damage in rheumatoid arthritis.
Proc Natl Acad Sci U S A. 112 (37): 11618-23.
Buján, J. et al. (2003) Expression of elastic components in healthy and varicose veins.
World J Surg. 27 (8): 901-5.
Korkmaz, B. et al. (2005) Inhibition of neutrophil elastase by alpha1-protease inhibitor at the surface of human polymorphonuclear neutrophils.
J Immunol. 175 (5): 3329-38.