Cytokeratin 19 antibody | A53-B/A2

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Mouse anti Human Cytokeratin 19

Product Type
Monoclonal Antibody
Clone
A53-B/A2
Isotype
IgG2a
Product Code Applications Pack Size List Price Quantity
5552-9009
Datasheet
E IF P* WB
MSDS
0.2 mg loader

Mouse anti Human cytokeratin 19 antibody, clone A53-B/A2 recognizes the rod domain (aa 312-335) of human cytokeratin 19 (Böttger; et al. 1995) also known as keratin 19 encoded by the KRT19 gene. Cytokeratin 19 is a 400 amino acid intermediate filament protein lacking a C-terminal tail domain, in contrast to all other intermediate filament proteins.

Cytokeratin 19 expression is observed in striated muscle where it is involved in forming the association between the contractile apparatus and dystrophin (Stone et al. 2005). Expression is also seen in many ductal and glandular cells together with a restricted set of normal and neoplasic epithelial cells.

Mouse anti Human cytokeratin 19 antibody, clone A53-B/A2 detects a band of ~45 kDa in western blotting using an A-549 human alveolar adenocarcinoma cell line lysate.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Human breast cancer cell line MCF-7.
Approx. Protein Concentrations
1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody.
Guarantee
18 months from date of despatch.

More Information

UniProt
P08727 Related reagents
Entrez Gene
KRT19 Related reagents
Regulatory
For research purposes only

Applications of Cytokeratin 19 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Immunofluorescence
Immunohistology - Paraffin 1 1/100 1/200
Western Blotting
  1. 1This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Histology Positive Control Tissue
Appendix
Western Blotting
In Western blotting 40 kD and 19 kD bands are observed.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Human anti Mouse IgG2a:FITC HCA037F F 0.1 mg loader
Human anti Mouse IgG2a:HRP HCA037P E 0.1 mg loader
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Product Specific References

References for Cytokeratin 19 antibody

  1. Karsten, U. et al. (1985) Monoclonal anti-cytokeratin antibody from a hybridoma clone generated by electrofusion.
    Eur J Cancer Clin Oncol. 21 (6): 733-40.
  2. Kasper, M. et al. (1987) Histological evaluation of three new monoclonal anti-cytokeratin antibodies. 1. Normal tissues.
    Eur J Cancer Clin Oncol. 23 (2): 137-47.
  3. Goletz, S. et al. (1997) Novel alphaGalNAc containing glycans on cytokeratins are recognized invitro by galectins with type II carbohydrate recognition domains.
    J Cell Sci. 110 ( Pt 14): 1585-96.
  4. de Neergaard, M. et al. (2010) Epithelial-stromal interaction 1 (EPSTI1) substitutes for peritumoral fibroblasts in the tumor microenvironment.
    Am J Pathol. 176: 1229-40.
  5. Ehlicke, F. et al. (2010) Intervertebral disc regeneration: influence of growth factors on differentiation of human mesenchymal stem cells (hMSC).
    Int J Artif Organs. 33 (4): 244-52.
  6. Johnston, R.L. et al. (2016) High content screening application for cell-type specific behaviour in heterogeneous primary breast epithelial subpopulations.
    Breast Cancer Res. 18 (1): 18.