Cortisol antibody | 137.5A12F
Mouse anti cortisol antibody, clone 137.5A12F, recognizes cortisol, a glucocorticoid hormone involved in responses to stress.
Cross reactivity was determined by competitive ELISA according to CLSI EP07A2 guidelines.
Cross reactivity was determined by competitive ELISA according to CLSI EP07A2 guidelines.
| Amount of Prednisone added | Cross reactivity |
| 36,040 ng/mL | 0.05% |
| 3,604 ng/mL | 0.4% |
| 360 ng/mL | 0.8% |
| 36 ng/mL | 1.6% |
| Amount of Prednisolone added | Cross reactivity |
| 36,040 ng/mL | 0.0% |
| 3,604 ng/mL | 0.2% |
| 360 ng/mL | 0.04% |
| 36 ng/mL | 4.0% |
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Human Cortisol
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- Regulatory
- For research purposes only
Applications of Cortisol antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 1/1000 | 1/10000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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