Mouse anti cortisol antibody, clone 137.5A12F, recognizes cortisol, a glucocorticoid hormone involved in responses to stress.
Cross reactivity was determined by competitive ELISA according to CLSI EP07A2 guidelines.
| Amount of Prednisone added | Cross reactivity |
| 36,040 ng/mL | 0.05% |
| 3,604 ng/mL | 0.4% |
| 360 ng/mL | 0.8% |
| 36 ng/mL | 1.6% |
| Amount of Prednisolone added | Cross reactivity |
| 36,040 ng/mL | 0.0% |
| 3,604 ng/mL | 0.2% |
| 360 ng/mL | 0.04% |
| 36 ng/mL | 4.0% |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Human Cortisol
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- Shelf Life
- 18 months from date of despatch.
- Regulatory
- For research purposes only
Applications of Cortisol antibody
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name |
Verified |
Min Dilution |
Max Dilution |
| ELISA |
|
1/1000 |
1/10000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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Secondary Antibodies Available
