Corticotrophin Releasing Factor antibody | 5D5
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Mouse anti Human Corticotropin Releasing Factor
- Product Type
- Monoclonal Antibody
- Clone
- 5D5
- Isotype
- IgG1
- Specificity
- Corticotrophin Releasing Factor
Antibody Quality and 
Unrivaled 
| Mouse anti Human corticotrophin-releasing factor antibody, clone 5D5 recognizes human corticotropin-releasing factor (CRF), a secreted hormone which regulates the release of corticotropin from the pituitary. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rat Expected from Sequence - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from ascites
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- Synthetic human corticotropin releasing factor.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
||
| Immunohistology - Paraffin 1 | |
1 | 10ug/ml |
- 1This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase.
*This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
- Histology Positive Control Tissue
- Human hypothalamus
References for Corticotrophin Releasing Factor antibody
-
Kravchenko, I. V. & Furalev, V. A. (1994) Monoclonal antibodies directed against two different corticotropin-releasing factor determinants
Hybridoma 13: 59 -64
- Synonyms
- CRF
- RRID
- AB_620121
- UniProt
- P06850
- Entrez Gene
- CRH
- GO Terms
- GO:0005576 extracellular region
- GO:0007165 signal transduction
- GO:0005184 neuropeptide hormone activity
- GO:0005625 soluble fraction
- GO:0007268 synaptic transmission
- GO:0007565 female pregnancy
- GO:0007567 parturition
- GO:0010841 positive regulation of circadian sleep/wake cycle, wakefulness
- GO:0042322 negative regulation of circadian sleep/wake cycle, REM sleep
- View More GO Terms
- GO:0051464 positive regulation of cortisol secretion
View more products with CORTICOTROPHIN RELEASING FACTOR specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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