Complement Factor P antibody | 062-27.8.1 (10-18)
Human serum was run under reducing conditions on SDS PAGE and transferred to a PVDF membrane. In lane 1, Bio-Rad Precision Plus molecular weight marker was run. In lane 2, Mouse anti Human Factor P (MCA2617) was used as the primary antibody at a dilution of 1/200. HRP conjugated Goat anti Mouse IgG (H/L) (STAR117P) was used at a dilution of 1/10,000 as the secondary antibody. As a control, HRP conjugated Goat anti Mouse IgG (H/L) (STAR117P) was run in lane 3. Visualization was carried out using the Bio-Rad ChemiDoc Imaging System with automatic exposure.
Mouse anti Human Factor P
- Product Type
- Monoclonal Antibody
- Clone
- 062-27.8.1 (10-18)
- Isotype
- IgG1
- Specificity
- Complement Factor P
Antibody Quality and Performance Guarantee
| Mouse anti Human Factor P antibody, clone 062-27.8.1 (10-18) recognizes human factor P, a 53 kDa glycosylated protein present in blood serum. Factor P binds to microbial surfaces and binds and stabilizes the complement component 3- and complement component 5-convertase enzyme complexes. C3b then acts as an opsonin or initiates the complement pathway again creating a positive feedback loop which acts only on the bacterial cell, leaving the host cells unharmed. Deficiency of factor P makes the host more susceptible to bacterial infections, particularly to Neisseria species and meningococcal infections. Mouse anti Human Factor P antibody, clone 10-18 is reported to inhibit the function of factor P. Removal of sodium azide is recommended prior to use in functional assays. |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from ascites
- Buffer Solution
- Borate buffered saline
- Preservative Stabilisers
- 0.1% Sodium Azide (NaN3)
- Immunogen
- Native purified factor P from human serum.
- Approx. Protein Concentrations
- Current, batch-specific concentration 1.086 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- Guaranteed until date of expiry. Please see product label.
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
||
| Flow Cytometry | |
1:1000 | |
| Functional Assays | |
||
| Immunohistology - Frozen | |
1:1000 | |
| Western Blotting | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Kidney from post streptoccal glomerulonephritis patients
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
|
Log in | ||
| List Price | Your Price | ||||||
|
|
Log in | ||||||
| Description | Mouse IgG1 Negative Control | ||||||
Further Reading
-
Hourcade, D.E. (2006) The role of properdin in the assembly of the alternative pathway C3 convertases of complement.
J Biol Chem. 281 (4): 2128-32. -
Jelezarova, E. et al. (2000) Interaction of C3b(2)--IgG complexes with complement proteins properdin, factor B and factor H: implications for amplification.
Biochem J. 349 (Pt 1): 217-23.
- Synonyms
- Properdin
- RRID
- AB_844542
- UniProt
- P27918
- Entrez Gene
- CFP
- GO Terms
- GO:0042742 defense response to bacterium
- GO:0005615 extracellular space
- GO:0006957 complement activation, alternative pathway
MCA2617
156229
159373
168345
168704
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
View more products with COMPLEMENT FACTOR specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
View all Anti-Human ProductsAlways be the first to know.
When we launch new products and resources to help you achieve more in the lab.
Yes, sign me up