Complement Factor B antibody | 014III-33.2.4.3
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Mouse anti Human Complement Factor B (Ba Fragment) antibody, clone 014III-33.2.4.3 recognises the 30 kDa Ba fragment of 90 kDa human complement factor B, present in blood serum. C3b associates with complement factor B, inducing conformational change. This enables complement factor D to cleave the N-terminal of complement factor B (the Ba subunit), leaving the 63 kDa Bb subunit associated with C3b, forming C3 convertase. Subunit Ba inhibits lymphocyte proliferation. Conversely, subunit Bb is involved in the proliferation of preactivated B lymphocytes.
Mouse anti Human Complement Factor B (Ba Fragment) antibody, clone 014III-33.2.4.3 has been reported for use in functional studies to block activity of the target protein. Removal of Sodium Azide is recommended prior to use in functional assays. Bio-Rad recommend the use of EQU003 for this purpose.
Mouse anti Human Complement Factor B (Ba Fragment) antibody, clone 014III-33.2.4.3 has been reported for use in functional studies to block activity of the target protein. Removal of Sodium Azide is recommended prior to use in functional assays. Bio-Rad recommend the use of EQU003 for this purpose.
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Borate buffered saline
- Preservative Stabilisers
- 0.1% Sodium Azide (NaN3)
- Immunogen
- Purified human complement factor b.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- Please see label for expiry date
More Information
- UniProt
- P00751 Related reagents
- Entrez Gene
- CFB Related reagents
- Regulatory
- For research purposes only
Applications of Complement Factor B antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA 1 | |||
| Flow Cytometry | |||
| Functional Assays | |||
| Immunohistology - Frozen | |||
| Western Blotting |
- 1Cone 014III-33.2.4.3binds both the Ba fragment and the whole protein so is not suitable for all ELISA applications.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Kidney from post streptococcal glomerulonephritis patients
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | 100 Tests | F |
Useful Reagents Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Histar Detection System | STAR3000A | 50 Tests | C P | ||
| Histar Detection System | STAR3000B | 150 Tests | C P | ||
| Histar Detection System | STAR3000C | 500 Tests | C P |
Product Specific References
References for Complement Factor B antibody
-
Zipplies, J.K. et al. (2010) Complement factor B expression profile in a spontaneous uveitis model.
Immunobiology. 215 (12): 949-55.
Further Reading
-
Xu, Y. et al. (2001) Structural biology of the alternative pathway convertase.
Immunol Rev. 180: 123-35. -
Ponnuraj, K. et al. (2004) Structural analysis of engineered Bb fragment of complement factor B: insights into the activation mechanism of the alternative pathway C3-convertase.
Mol Cell. 14 (1): 17-28.
