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Complement Factor B antibody | 014III-33.2.4.3

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Anti Human Complement Factor B (Ba Fragment) Antibody, clone 014III-33.2.4.3 gallery image 1
Published customer image:
Mouse anti Human complement factor B antibody, clone 014III-33.2.4.3 (MCA2647) used to detect Complement factor B expressing cells in photoreceptor layers of the eye by immunohistochemistry on cryostat sections.
Image caption:
Presence of CFB immunoreactivity in the photoreceptor outer segment layer of donor eyes diagnosed with GA
(a) Immunohistochemical detection CFB (red) in a 67 year old female control donor eye (left panel) and 82 (middle panel) and 88 (right panel) year old female donor eyes diagnosed with GA. CFB is detected in the choroid of all donor eyes, and in the photoreceptor outer segment layer of donor eyes diagnosed with GA.
(b) Immunohistochemistry results on two serial sections obtained from the GA donor eye shown in the middle panel of (a). In the left panel, the primary antibody has been replaced with a mouse IgG2a isotype control antibody (isotype). The right panels show sections through human liver stained with a-CFB antibody or with isotype control antibody. GCL = granule cell layer, INL = inner nuclear layer, BM = Bruch's membrane. Scale bars = 50μm (a, left panels in b) or 10μm (right panel in b).

From: Katschke KJ Jr, Xi H, Cox C, Truong T, Malato Y, Lee WP, McKenzie B, Arceo R, Tao J, Rangell L, Reichelt M, Diehl L, Elstrott J, Weimer RM, van Lookeren Campagne M.
Classical and alternative complement activation on photoreceptor outer segments drives monocyte-dependent retinal atrophy.
Sci Rep. 2018 May 9;8(1):7348.
doi: 10.1038/s41598-018-25557-8.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Mouse anti Human Complement Factor B (Ba Fragment)

Product Type
Monoclonal Antibody
Clone
014III-33.2.4.3
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA2647
Datasheet
C E* F FN WB
SDS
0.1 ml loader

Mouse anti Human Complement Factor B (Ba Fragment) antibody, clone 014III-33.2.4.3 recognises the 30 kDa Ba fragment of 90 kDa human complement factor B, present in blood serum. C3b associates with complement factor B, inducing conformational change. This enables complement factor D to cleave the N-terminal of complement factor B (the Ba subunit), leaving the 63 kDa Bb subunit associated with C3b, forming C3 convertase. Subunit Ba inhibits lymphocyte proliferation. Conversely, subunit Bb is involved in the proliferation of preactivated B lymphocytes.

Mouse anti Human Complement Factor B (Ba Fragment) antibody, clone 014III-33.2.4.3 has been reported for use in functional studies to block activity of the target protein. Removal of Sodium Azide is recommended prior to use in functional assays. Bio-Rad recommend the use of EQU003 for this purpose.

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Borate buffered saline
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
Immunogen
Purified human complement factor b.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
Guaranteed until date of expiry. Please see product label.

More Information

UniProt
P00751
Entrez Gene
CFB
Regulatory
For research purposes only

Applications of Complement Factor B antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1
Flow Cytometry
Functional Assays
Immunohistology - Frozen
Western Blotting
  1. 1Cone 014III-33.2.4.3binds both the Ba fragment and the whole protein so is not suitable for all ELISA applications.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Kidney from post streptococcal glomerulonephritis patients
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Mouse IgG1 Negative Control MCA928 F 100 Tests loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Histar Detection System STAR3000A C P 50 Tests

Product Specific References

References for Complement Factor B antibody

  1. Zipplies, J.K. et al. (2010) Complement factor B expression profile in a spontaneous uveitis model.
    Immunobiology. 215 (12): 949-55.
  2. Katschke, K.J. Jr. et al. (2018) Classical and alternative complement activation on photoreceptor outer segments drives monocyte-dependent retinal atrophy.
    Sci Rep. 8 (1): 7348.
  3. Loyet, K.M. et al. (2012) Activation of the alternative complement pathway in vitreous is controlled by genetics in age-related macular degeneration.
    Invest Ophthalmol Vis Sci. 53 (10): 6628-37.

Further Reading

  1. Xu, Y. et al. (2001) Structural biology of the alternative pathway convertase.
    Immunol Rev. 180: 123-35.
  2. Ponnuraj, K. et al. (2004) Structural analysis of engineered Bb fragment of complement factor B: insights into the activation mechanism of the alternative pathway C3-convertase.
    Mol Cell. 14 (1): 17-28.

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