Mouse anti Human Complement Factor B (Ba Fragment) antibody, clone 014III-220.127.116.11
recognises the 30 kDa Ba fragment of 90 kDa human complement factor B, present in blood serum. C3b associates with complement factor B, inducing conformational change. This enables complement factor D to cleave the N-terminal of complement factor B (the Ba subunit), leaving the 63 kDa Bb subunit associated with C3b, forming C3 convertase. Subunit Ba inhibits lymphocyte proliferation. Conversely, subunit Bb is involved in the proliferation of preactivated B lymphocytes.
Mouse anti Human Complement Factor B (Ba Fragment) antibody, clone 014III-18.104.22.168 has been reported for use in functional studies to block activity of the target protein. Removal of Sodium Azide is recommended prior to use in functional assays. Bio-Rad recommend the use of EQU003
for this purpose.
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Borate buffered saline
- Preservative Stabilisers
- 0.1% Sodium Azide (NaN3)
- Purified human complement factor b.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- Guaranteed until date of expiry. Please see product label.
- Entrez Gene
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of Complement Factor B antibody
|Immunohistology - Frozen
- 1Cone 014III-22.214.171.124binds both the Ba fragment and the whole protein so is not suitable for all ELISA applications.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Kidney from post streptococcal glomerulonephritis patients
Copyright © 2020 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
Useful Reagents Available
Product Specific References
References for Complement Factor B antibody
Zipplies, J.K. et al. (2010) Complement factor B expression profile in a spontaneous uveitis model.
Immunobiology. 215 (12): 949-55.
Katschke, K.J. Jr. et al. (2018) Classical and alternative complement activation on photoreceptor outer segments drives monocyte-dependent retinal atrophy.
Sci Rep. 8 (1): 7348.
Loyet, K.M. et al. (2012) Activation of the alternative complement pathway in vitreous is controlled by genetics in age-related macular degeneration.
Invest Ophthalmol Vis Sci. 53 (10): 6628-37.
Xu, Y. et al. (2001) Structural biology of the alternative pathway convertase.
Immunol Rev. 180: 123-35.
Ponnuraj, K. et al. (2004) Structural analysis of engineered Bb fragment of complement factor B: insights into the activation mechanism of the alternative pathway C3-convertase.
Mol Cell. 14 (1): 17-28.