Complement Factor B antibody | 014III-33.2.4.3
Mouse anti Human Complement Factor B (Ba Fragment) antibody, clone 014III-33.2.4.3 has been reported for use in functional studies to block activity of the target protein. Removal of Sodium Azide is recommended prior to use in functional assays. Bio-Rad recommend the use of EQU003 for this purpose.
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Borate buffered saline
- Preservative Stabilisers
- 0.1% Sodium Azide (NaN3)
- Immunogen
- Purified human complement factor b.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- Guaranteed until date of expiry. Please see product label.
More Information
Applications of Complement Factor B antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA 1 | |||
Flow Cytometry | |||
Functional Assays | |||
Immunohistology - Frozen | |||
Western Blotting |
- 1Cone 014III-33.2.4.3binds both the Ba fragment and the whole protein so is not suitable for all ELISA applications.
- Histology Positive Control Tissue
- Kidney from post streptococcal glomerulonephritis patients
Secondary Antibodies Available
Negative Isotype Controls Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
![]() |
Useful Reagents Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Histar Detection System | STAR3000A | C P | 50 Tests |
Product Specific References
References for Complement Factor B antibody
-
Zipplies, J.K. et al. (2010) Complement factor B expression profile in a spontaneous uveitis model.
Immunobiology. 215 (12): 949-55. -
Katschke, K.J. Jr. et al. (2018) Classical and alternative complement activation on photoreceptor outer segments drives monocyte-dependent retinal atrophy.
Sci Rep. 8 (1): 7348. -
Loyet, K.M. et al. (2012) Activation of the alternative complement pathway in vitreous is controlled by genetics in age-related macular degeneration.
Invest Ophthalmol Vis Sci. 53 (10): 6628-37.
Further Reading
-
Xu, Y. et al. (2001) Structural biology of the alternative pathway convertase.
Immunol Rev. 180: 123-35. -
Ponnuraj, K. et al. (2004) Structural analysis of engineered Bb fragment of complement factor B: insights into the activation mechanism of the alternative pathway C3-convertase.
Mol Cell. 14 (1): 17-28.
Fluorescent Spectraviewer
Watch the Tool Tutorial Video ▸
How to Use the Spectraviewer?
Watch the Tool Tutorial Video ▸
- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra