Complement Factor B antibody | 014III-33.2.4.3

Mouse anti Human Complement Factor B (Ba Fragment)

Product Type
Monoclonal Antibody
Clone
014III-33.2.4.3
Isotype
IgG1
Specificity
Complement Factor B
Region
(Ba FRAGMENT)

Product Code Applications Pack Size List Price Your Price Qty
MCA2647
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C E * F FN WB 0.1 ml
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Mouse anti Human Complement Factor B (Ba Fragment) antibody, clone 014III-33.2.4.3 recognises the 30 kDa Ba fragment of 90 kDa human complement factor B, present in blood serum. C3b associates with complement factor B, inducing conformational change. This enables complement factor D to cleave the N-terminal of complement factor B (the Ba subunit), leaving the 63 kDa Bb subunit associated with C3b, forming C3 convertase. Subunit Ba inhibits lymphocyte proliferation. Conversely, subunit Bb is involved in the proliferation of preactivated B lymphocytes.

Mouse anti Human Complement Factor B (Ba Fragment) antibody, clone 014III-33.2.4.3 blocks activity of the target protein. Removal of Sodium Azide is recommended prior to use in functional assays.

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from ascites
Buffer Solution
Borate buffered saline
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
Immunogen
Purified human complement factor b.
Approx. Protein Concentrations
Current, batch-specific concentration 1.1 mg/ml
Regulatory
For research purposes only
Guarantee
Guaranteed until date of expiry. Please see product label.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1
Flow Cytometry
Functional Assays
Immunohistology - Frozen
Western Blotting
  1. 1Cone 014III-33.2.4.3binds both the Ba fragment and the whole protein so is not suitable for all ELISA applications.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Kidney from post streptococcal glomerulonephritis patients

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References for Complement Factor B antibody

  1. Zipplies, J.K. et al. (2010) Complement factor B expression profile in a spontaneous uveitis model.
    Immunobiology. 215 (12): 949-55.
  2. Katschke, K.J. Jr. et al. (2018) Classical and alternative complement activation on photoreceptor outer segments drives monocyte-dependent retinal atrophy.
    Sci Rep. 8 (1): 7348.
  3. Loyet, K.M. et al. (2012) Activation of the alternative complement pathway in vitreous is controlled by genetics in age-related macular degeneration.
    Invest Ophthalmol Vis Sci. 53 (10): 6628-37.

Further Reading

  1. Xu, Y. et al. (2001) Structural biology of the alternative pathway convertase.
    Immunol Rev. 180: 123-35.
  2. Ponnuraj, K. et al. (2004) Structural analysis of engineered Bb fragment of complement factor B: insights into the activation mechanism of the alternative pathway C3-convertase.
    Mol Cell. 14 (1): 17-28.

RRID
AB_2080001
UniProt
P00751
Entrez Gene
CFB
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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