Complement Factor B antibody | 014III-33.2.4.3
Mouse anti Human complement factor B antibody, clone 014III-33.2.4.3 (MCA2647) used to detect Complement factor B expressing cells in photoreceptor layers of the eye by immunohistochemistry on cryostat sections.
Image caption:
Presence of CFB immunoreactivity in the photoreceptor outer segment layer of donor eyes diagnosed with GA
(a) Immunohistochemical detection CFB (red) in a 67 year old female control donor eye (left panel) and 82 (middle panel) and 88 (right panel) year old female donor eyes diagnosed with GA. CFB is detected in the choroid of all donor eyes, and in the photoreceptor outer segment layer of donor eyes diagnosed with GA.
(b) Immunohistochemistry results on two serial sections obtained from the GA donor eye shown in the middle panel of (a). In the left panel, the primary antibody has been replaced with a mouse IgG2a isotype control antibody (isotype). The right panels show sections through human liver stained with a-CFB antibody or with isotype control antibody. GCL = granule cell layer, INL = inner nuclear layer, BM = Bruch's membrane. Scale bars = 50μm (a, left panels in b) or 10μm (right panel in b).
From: Katschke KJ Jr, Xi H, Cox C, Truong T, Malato Y, Lee WP, McKenzie B, Arceo R, Tao J, Rangell L, Reichelt M, Diehl L, Elstrott J, Weimer RM, van Lookeren Campagne M.
Classical and alternative complement activation on photoreceptor outer segments drives monocyte-dependent retinal atrophy.
Sci Rep. 2018 May 9;8(1):7348.
doi: 10.1038/s41598-018-25557-8.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Human Complement Factor B (Ba Fragment) antibody, clone 014III-33.2.4.3 recognises the 30 kDa Ba fragment of 90 kDa human complement factor B, present in blood serum. C3b associates with complement factor B, inducing conformational change. This enables complement factor D to cleave the N-terminal of complement factor B (the Ba subunit), leaving the 63 kDa Bb subunit associated with C3b, forming C3 convertase. Subunit Ba inhibits lymphocyte proliferation. Conversely, subunit Bb is involved in the proliferation of preactivated B lymphocytes.
Mouse anti Human Complement Factor B (Ba Fragment) antibody, clone 014III-33.2.4.3 has been reported for use in functional studies to block activity of the target protein. Removal of Sodium Azide is recommended prior to use in functional assays. Bio-Rad recommend the use of EQU003 for this purpose.
Mouse anti Human Complement Factor B (Ba Fragment) antibody, clone 014III-33.2.4.3 has been reported for use in functional studies to block activity of the target protein. Removal of Sodium Azide is recommended prior to use in functional assays. Bio-Rad recommend the use of EQU003 for this purpose.
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Borate buffered saline
- Preservative Stabilisers
- 0.1% Sodium Azide (NaN3)
- Immunogen
- Purified human complement factor b.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- Guaranteed until date of expiry. Please see product label.
More Information
Applications of Complement Factor B antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA 1 | |||
| Flow Cytometry | |||
| Functional Assays | |||
| Immunohistology - Frozen | |||
| Western Blotting |
- 1Cone 014III-33.2.4.3binds both the Ba fragment and the whole protein so is not suitable for all ELISA applications.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Kidney from post streptococcal glomerulonephritis patients
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Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
|
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Histar Detection System | STAR3000A | C P | 50 Tests |
Product Specific References
References for Complement Factor B antibody
-
Zipplies, J.K. et al. (2010) Complement factor B expression profile in a spontaneous uveitis model.
Immunobiology. 215 (12): 949-55. -
Katschke, K.J. Jr. et al. (2018) Classical and alternative complement activation on photoreceptor outer segments drives monocyte-dependent retinal atrophy.
Sci Rep. 8 (1): 7348. -
Loyet, K.M. et al. (2012) Activation of the alternative complement pathway in vitreous is controlled by genetics in age-related macular degeneration.
Invest Ophthalmol Vis Sci. 53 (10): 6628-37.
Further Reading
-
Xu, Y. et al. (2001) Structural biology of the alternative pathway convertase.
Immunol Rev. 180: 123-35. -
Ponnuraj, K. et al. (2004) Structural analysis of engineered Bb fragment of complement factor B: insights into the activation mechanism of the alternative pathway C3-convertase.
Mol Cell. 14 (1): 17-28.
