Complement Factor B antibody | 014III-33.2.4.3
Mouse anti Human complement factor B antibody, clone 014III-33.2.4.3 (MCA2647) used to detect Complement factor B expressing cells in photoreceptor layers of the eye by immunohistochemistry on cryostat sections.
Image caption:
Presence of CFB immunoreactivity in the photoreceptor outer segment layer of donor eyes diagnosed with GA
(a) Immunohistochemical detection CFB (red) in a 67 year old female control donor eye (left panel) and 82 (middle panel) and 88 (right panel) year old female donor eyes diagnosed with GA. CFB is detected in the choroid of all donor eyes, and in the photoreceptor outer segment layer of donor eyes diagnosed with GA.
(b) Immunohistochemistry results on two serial sections obtained from the GA donor eye shown in the middle panel of (a). In the left panel, the primary antibody has been replaced with a mouse IgG2a isotype control antibody (isotype). The right panels show sections through human liver stained with a-CFB antibody or with isotype control antibody. GCL = granule cell layer, INL = inner nuclear layer, BM = Bruch's membrane. Scale bars = 50μm (a, left panels in b) or 10μm (right panel in b).
From: Katschke KJ Jr, Xi H, Cox C, Truong T, Malato Y, Lee WP, McKenzie B, Arceo R, Tao J, Rangell L, Reichelt M, Diehl L, Elstrott J, Weimer RM, van Lookeren Campagne M.
Classical and alternative complement activation on photoreceptor outer segments drives monocyte-dependent retinal atrophy.
Sci Rep. 2018 May 9;8(1):7348.
doi: 10.1038/s41598-018-25557-8.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Human Complement Factor B (Ba Fragment)
- Product Type
- Monoclonal Antibody
- Clone
- 014III-33.2.4.3
- Isotype
- IgG1
- Specificity
- Complement Factor B
- Region
- (Ba FRAGMENT)
Antibody Quality and Performance Guarantee
| Mouse anti Human Complement Factor B (Ba Fragment) antibody, clone 014III-33.2.4.3 recognises the 30 kDa Ba fragment of 90 kDa human complement factor B, present in blood serum. C3b associates with complement factor B, inducing conformational change. This enables complement factor D to cleave the N-terminal of complement factor B (the Ba subunit), leaving the 63 kDa Bb subunit associated with C3b, forming C3 convertase. Subunit Ba inhibits lymphocyte proliferation. Conversely, subunit Bb is involved in the proliferation of preactivated B lymphocytes.
Mouse anti Human Complement Factor B (Ba Fragment) antibody, clone 014III-33.2.4.3 blocks activity of the target protein. Removal of Sodium Azide is recommended prior to use in functional assays. |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from ascites
- Buffer Solution
- Borate buffered saline
- Preservative Stabilisers
- <0.1% sodium azide (NaN3)
- Immunogen
- Purified human complement factor b.
- Approx. Protein Concentrations
- Current, batch-specific concentration 1.1 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- Guaranteed until date of expiry. Please see product label.
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA 1 |  |
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| Flow Cytometry | |
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| Functional Assays | |
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| Immunohistology - Frozen | |
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| Western Blotting | |
- 1Cone 014III-33.2.4.3binds both the Ba fragment and the whole protein so is not suitable for all ELISA applications.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Kidney from post streptococcal glomerulonephritis patients
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control | ||||||
References for Complement Factor B antibody
-
Zipplies, J.K. et al. (2010) Complement factor B expression profile in a spontaneous uveitis model.
Immunobiology. 215 (12): 949-55. -
Loyet, K.M. et al. (2012) Activation of the alternative complement pathway in vitreous is controlled by genetics in age-related macular degeneration.
Invest Ophthalmol Vis Sci. 53 (10): 6628-37. -
Katschke, K.J. Jr. et al. (2018) Classical and alternative complement activation on photoreceptor outer segments drives monocyte-dependent retinal atrophy.
Sci Rep. 8 (1): 7348.
Further Reading
-
Xu, Y. et al. (2001) Structural biology of the alternative pathway convertase.
Immunol Rev. 180: 123-35. -
Ponnuraj, K. et al. (2004) Structural analysis of engineered Bb fragment of complement factor B: insights into the activation mechanism of the alternative pathway C3-convertase.
Mol Cell. 14 (1): 17-28.
- RRID
- AB_2080001
- UniProt
- P00751
- Entrez Gene
- CFB
MCA2647
156700
160625
164602
168608
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