Mouse anti Human clusterin antibody, clone 3R3/2 recognizes clusterin, an ubiquitously expressed protein of two 40 kDa chains, alpha and beta, covalently joined by disulfide bonds. It exists in two forms; a pro-apoptotic form localised to the nucleus and a pro-survival secreted form.
Both are thought to be involved in DNA repair and cell cycle regulation. The secreted form is thought to inhibit apoptotic signalling by BAX, Clusterin suppression can sensitise cells to chemotherapy. Clusterin is overexpressed in human prostate cancer, breast cancers and squamous cell carcinomas.
Part of the SC5b-9 complex, clusterin prevents the binding of a C5b-C7 complex to the membrane of the target cell inhibiting the complement cascade. Clusterin acts as an extracellular chaperone inhibiting the formation of human lysozyme amyloid associated with Alzheimer's disease. Clusterin is also important in spermatogenesis and is secreted by Sertoli cells.
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Borate buffered saline
- Preservative Stabilisers
- 0.1% Sodium Azide (NaN3)
- Glomerular basement membrane from a post-mortem kidney.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 18 months from date of despatch.
- Entrez Gene
- GO Terms
release of cytochrome c from mitochondria
response to virus
lipid metabolic process
complement activation, classical pathway
misfolded protein binding
platelet alpha granule lumen
negative regulation of protein homooligomerization
spherical high-density lipoprotein particle
reverse cholesterol transport
innate immune response
response to misfolded protein
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of Clusterin antibody
|Immunohistology - Frozen
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Kidney from post streptocial glomerulonephritis patients.
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Secondary Antibodies Available
Negative Isotype Controls Available
Product Specific References
References for Clusterin antibody
Kumita, J.R. et al. (2007) The extracellular chaperone clusterin potently inhibits human lysozyme amyloid formation by interacting with prefibrillar species.
J Mol Biol. 369 (1): 157-67.
Shannan, B. et al. (2006) Clusterin and DNA repair: a new function in cancer for a key player in apoptosis and cell cycle control.
J Mol Histol. 37 (5-7): 183-8.