Clusterin antibody | 3R3/2
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Mouse anti Human Clusterin
- Product Type
- Monoclonal Antibody
- Clone
- 3R3/2
- Isotype
- IgG1
- Specificity
- Clusterin
Antibody Quality and 
Unrivaled 
| Mouse anti Human clusterin antibody, clone 3R3/2 recognizes clusterin, an ubiquitously expressed protein of two 40 kDa chains, alpha and beta, covalently joined by disulfide bonds. It exists in two forms; a pro-apoptotic form localised to the nucleus and a pro-survival secreted form.
Both are thought to be involved in DNA repair and cell cycle regulation. The secreted form is thought to inhibit apoptotic signalling by BAX, Clusterin suppression can sensitise cells to chemotherapy. Clusterin is overexpressed in human prostate cancer, breast cancers and squamous cell carcinomas. Part of the SC5b-9 complex, clusterin prevents the binding of a C5b-C7 complex to the membrane of the target cell inhibiting the complement cascade. Clusterin acts as an extracellular chaperone inhibiting the formation of human lysozyme amyloid associated with Alzheimer's disease. Clusterin is also important in spermatogenesis and is secreted by Sertoli cells. |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Borate buffered saline
- Preservative Stabilisers
- 0.1% Sodium Azide (NaN3)
- Immunogen
- Glomerular basement membrane from a post-mortem kidney.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- Guaranteed until date of expiry. Please see product label.
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
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| Flow Cytometry | |
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| Immunohistology - Frozen | |
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| Western Blotting | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Kidney from post streptocial glomerulonephritis patients.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control | ||||||
References for Clusterin antibody
-
Kumita, J.R. et al. (2007) The extracellular chaperone clusterin potently inhibits human lysozyme amyloid formation by interacting with prefibrillar species.
J Mol Biol. 369 (1): 157-67. -
Shannan, B. et al. (2006) Clusterin and DNA repair: a new function in cancer for a key player in apoptosis and cell cycle control.
J Mol Histol. 37 (5-7): 183-8.
- Synonyms
- Apolipoprotein J
- RRID
- AB_2260877
- UniProt
- P10909
- Entrez Gene
- CLU
- GO Terms
- GO:0001836 release of cytochrome c from mitochondria
- GO:0002576 platelet degranulation
- GO:0009615 response to virus
- GO:0005739 mitochondrion
- GO:0006629 lipid metabolic process
- GO:0006958 complement activation, classical pathway
- GO:0051787 misfolded protein binding
- GO:0030168 platelet activation
- GO:0031093 platelet alpha granule lumen
- View More GO Terms
- GO:0032463 negative regulation of protein homooligomerization
- GO:0034366 spherical high-density lipoprotein particle
- GO:0043691 reverse cholesterol transport
- GO:0045087 innate immune response
- GO:0051788 response to misfolded protein
View more products with CLUSTERIN specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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