Chromogranin A antibody | LK2H10

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Anti Human Chromogranin A Antibody, clone LK2H10 thumbnail image 1

Anti Human Chromogranin A Antibody, clone LK2H10 thumbnail image 2

Anti Human Chromogranin A Antibody, clone LK2H10 thumbnail image 3

Anti Human Chromogranin A Antibody, clone LK2H10 thumbnail image 4

Anti Human Chromogranin A Antibody, clone LK2H10 gallery image 1 — Citrate pretreated paraffin embedded human pancreas stained with Mouse anti Human Chromogranin A (**MCA5696**) followed by Histar Detection (STAR3000A)

Anti Human Chromogranin A Antibody, clone LK2H10 gallery image 2 — Immunoperoxidase staining of a human pancreas cryosection with Mouse anti Human chromogranin A antibody, clone LK2H10 (**MCA5696**) followed by Histar detection system (STAR3000). Low power

Anti Human Chromogranin A Antibody, clone LK2H10 gallery image 3 — Immunoperoxidase staining of a human pancreas cryosection with Mouse anti Human chromogranin A antibody, clone LK2H10 (**MCA5696**) followed by Histar detection systm (STAR3000). Medium power

Anti Human Chromogranin A Antibody, clone LK2H10 gallery image 4 — Immunoperoxidase staining of a human pancreas cryosection with Mouse anti Human chromogranin A antibody, clone LK2H10 (**MCA5696**) followed by Histar detection systm (STAR3000). High power

Mouse anti Human Chromogranin A

Product Type: Monoclonal Antibody
Clone: LK2H10
Isotype: IgG1

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
MCA5696	C P* WB			0.2 mg

Mouse anti Human Chromogranin A antibody, clone LK2H10 specifically recognizes chromogranin A (CgA), a secretory glycoprotein and member of the chromogranin/secretogranin family, widely expressed by cells of the neuroendocrine system and related tumours, which is used in immunohistochemistry as a valuable diagnostic marker for pheochromocytomas, paragangliomas and carcinoid, pancreatic and prostate tumours.

CgA constitutes one of the most abundant components of secretory granules, and is the precursor to several functional peptides, including vasostatin and pancreastatin.

Clone LK2H10 has been shown to be suitable for use on cytospins (Jensen et al. 1990).

Product Details

Target Species

Human

Species Cross-Reactivity

Target Species	Cross Reactivity
Pig
Monkey

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Preparation

Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)

Carrier Free

Yes

Immunogen

Human pheochromocytoma tissue.

Approx. Protein Concentrations

IgG concentration 1.0mg/ml

Fusion Partners

Spleen cells from immunised Balb/c mice were fused with cells of the NS-1 mouse myeloma cell line.

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

More Information

UniProt: P10645 Related reagents; P26339 Related reagents; P04404 Related reagents
Entrez Gene: CHGA Related reagents; CHGA Related reagents; Chga Related reagents
GO Terms: GO:0005576 extracellular region; GO:0008217 regulation of blood pressure; GO:0005515 protein binding
Regulatory: For research purposes only

Applications of Chromogranin A antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
Immunohistology - Frozen
Immunohistology - Paraffin ¹	1/100	1/500
Western Blotting

¹This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Histology Positive Control Tissue: Normal Pancreas

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Application Based External Images

Immunohistology - Paraffin

Product Specific References

References for Chromogranin A antibody

Lloyd, R.V. & Wilson, B.S. (1983) Specific endocrine tissue marker defined by a monoclonal antibody.
Science. 222 (4624): 628-30.
DeStephano, D.B. et al. (1984) Pituitary adenomas. An immunohistochemical study of hormone production and chromogranin localization.
Am J Pathol. 116 (3): 464-72.
Jensen, S.M. et al. (1990) A comparison of synaptophysin, chromogranin, and L-dopa decarboxylase as markers for neuroendocrine differentiation in lung cancer cell lines.
Cancer Res. 50 (18): 6068-74.
Lloyd, R.V. et al. (1984) Immunohistochemical detection of chromogranin and neuron-specific enolase in pancreatic endocrine neoplasms.
Am J Surg Pathol. 8 (8): 607-14.
Walts, A.E. et al. (1985) Chromogranin as a marker of neuroendocrine cells in cytologic material--an immunocytochemical study.
Am J Clin Pathol. 84 (3): 273-7.
Wilson, B.S. & Lloyd, R.V. (1984) Detection of chromogranin in neuroendocrine cells with a monoclonal antibody.
Am J Pathol. 115 (3): 458-68.
Wilson, B.S. et al. (1986) Chromogranin from normal human adrenal glands: purification by monoclonal antibody affinity chromatography and partial N-terminal amino acid sequence.
Regul Pept. 13 (3-4): 207-23.
Galfré, G. et al. (1977) Antibodies to major histocompatibility antigens produced by hybrid cell lines.
Nature. 266 (5602): 550-2.
Pernicová, Z. et al. (2011) Androgen depletion induces senescence in prostate cancer cells through down-regulation of Skp2.
Neoplasia. 13: 526-36.
Endo, C. et al. (2009) Immunocytochemical evaluation of large cell neuroendocrine carcinoma of the lung.
Acta Cytol. 53: 36-40.
Jeong, S.et al. (2016) Distinct metaplastic and inflammatory phenotypes in autoimmune and adenocarcinoma-associated chronic atrophic gastritis
United European Gastroenterology Journal. Apr 11 [Epub ahead of print]
Nam, K.T. et al. (2012) Gastric tumor development in Smad3-deficient mice initiates from forestomach/glandular transition zone along the lesser curvature.
Lab Invest. 92 (6): 883-95.
O'Neal, R.L. et al. (2013) Human epididymis protein 4 is up-regulated in gastric and pancreatic adenocarcinomas.
Hum Pathol. 44 (5): 734-42.

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