Chromogranin A antibody | LK2H10
Mouse anti Human Chromogranin A
- Product Type
- Monoclonal Antibody
- Clone
- LK2H10
- Isotype
- IgG1
- Specificity
- Chromogranin A
Antibody Quality and Performance Guarantee
| Mouse anti Human Chromogranin A antibody, clone LK2H10 specifically recognizes chromogranin A (CgA), a secretory glycoprotein and member of the chromogranin/secretogranin family, widely expressed by cells of the neuroendocrine system and related tumours, which is used in immunohistochemistry as a valuable diagnostic marker for pheochromocytomas, paragangliomas and carcinoid, pancreatic and prostate tumours. CgA constitutes one of the most abundant components of secretory granules, and is the precursor to several functional peptides, including vasostatin and pancreastatin. Clone LK2H10 has been shown to be suitable for use on cytospins (Jensen et al. 1990). |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Pig Monkey - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Human pheochromocytoma tissue.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Fusion Partners
- Spleen cells from immunized Balb/c mice were fused with cells of the NS-1 mouse myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Frozen |  |
||
| Immunohistology - Paraffin 1 | |
1/100 | 1/500 |
| Western Blotting | |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Normal Pancreas
Source Reference
-
Wilson, B.S. & Lloyd, R.V. (1984) Detection of chromogranin in neuroendocrine cells with a monoclonal antibody.
Am J Pathol. 115 (3): 458-68.
Antibody Characterization Reference
-
DeStephano, D.B. et al. (1984) Pituitary adenomas. An immunohistochemical study of hormone production and chromogranin localization.
Am J Pathol. 116 (3): 464-72.
References for Chromogranin A antibody
-
Lloyd, R.V. & Wilson, B.S. (1983) Specific endocrine tissue marker defined by a monoclonal antibody.
Science. 222 (4624): 628-30. -
Lloyd, R.V. et al. (1984) Immunohistochemical detection of chromogranin and neuron-specific enolase in pancreatic endocrine neoplasms.
Am J Surg Pathol. 8 (8): 607-14. -
Walts, A.E. et al. (1985) Chromogranin as a marker of neuroendocrine cells in cytologic material--an immunocytochemical study.
Am J Clin Pathol. 84 (3): 273-7. -
Wilson, B.S. et al. (1986) Chromogranin from normal human adrenal glands: purification by monoclonal antibody affinity chromatography and partial N-terminal amino acid sequence.
Regul Pept. 13 (3-4): 207-23. -
Jensen, S.M. et al. (1990) A comparison of synaptophysin, chromogranin, and L-dopa decarboxylase as markers for neuroendocrine differentiation in lung cancer cell lines.
Cancer Res. 50 (18): 6068-74. -
Endo, C. et al. (2009) Immunocytochemical evaluation of large cell neuroendocrine carcinoma of the lung.
Acta Cytol. 53: 36-40. -
Pernicová, Z. et al. (2011) Androgen depletion induces senescence in prostate cancer cells through down-regulation of Skp2.
Neoplasia. 13: 526-36. -
Nam, K.T. et al. (2012) Gastric tumor development in Smad3-deficient mice initiates from forestomach/glandular transition zone along the lesser curvature.
Lab Invest. 92 (6): 883-95.
View The Latest Product References
-
O'Neal, R.L. et al. (2013) Human epididymis protein 4 is up-regulated in gastric and pancreatic adenocarcinomas.
Hum Pathol. 44 (5): 734-42. -
Jeong, S. et al. (2017) Distinct metaplastic and inflammatory phenotypes in autoimmune and adenocarcinoma-associated chronic atrophic gastritis.
United European Gastroenterol J. 5 (1): 37-44. -
Hopton, R.E. et al. (2023) The Role of Lrig1 in the Development of the Colonic Epithelium.
bioRxiv. May 02 [Preprint].
Further Reading
-
Galfré, G. et al. (1977) Antibodies to major histocompatibility antigens produced by hybrid cell lines.
Nature. 266 (5602): 550-2.
- RRID
- AB_10718986
- UniProt
- P10645
- P26339
- P04404
- Entrez Gene
- CHGA
- CHGA
- Chga
- GO Terms
- GO:0005576 extracellular region
- GO:0008217 regulation of blood pressure
- GO:0005515 protein binding
MCA5696
155117
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