Chromogranin A antibody | LK2H10

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Mouse anti Human Chromogranin A

Product Type
Monoclonal Antibody
Clone
LK2H10
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA5696 C P* WB 0.2 mg
Mouse anti Human Chromogranin A antibody, clone LK2H10 specifically recognizes chromogranin A (CgA), a secretory glycoprotein and member of the chromogranin/secretogranin family, widely expressed by cells of the neuroendocrine system and related tumours, which is used in immunohistochemistry as a valuable diagnostic marker for pheochromocytomas, paragangliomas and carcinoid, pancreatic and prostate tumours.

CgA constitutes one of the most abundant components of secretory granules, and is the precursor to several functional peptides, including vasostatin and pancreastatin.

Clone LK2H10 has been shown to be suitable for use on cytospins (Jensen et al. 1990).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Pig
Monkey
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Human pheochromocytoma tissue.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Fusion Partners
Spleen cells from immunised Balb/c mice were fused with cells of the NS-1 mouse myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P10645 Related reagents
P26339 Related reagents
P04404 Related reagents
Entrez Gene
CHGA Related reagents
CHGA Related reagents
Chga Related reagents
GO Terms
GO:0005576 extracellular region
GO:0008217 regulation of blood pressure
GO:0005515 protein binding
Regulatory
For research purposes only

Applications of Chromogranin A antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen
Immunohistology - Paraffin 1 1/100 1/500
Western Blotting
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Normal Pancreas

Application Based External Images

Immunohistology - Paraffin

Product Specific References

References for Chromogranin A antibody

  1. Lloyd, R.V. & Wilson, B.S. (1983) Specific endocrine tissue marker defined by a monoclonal antibody.
    Science. 222 (4624): 628-30.
  2. DeStephano, D.B. et al. (1984) Pituitary adenomas. An immunohistochemical study of hormone production and chromogranin localization.
    Am J Pathol. 116 (3): 464-72.
  3. Jensen, S.M. et al. (1990) A comparison of synaptophysin, chromogranin, and L-dopa decarboxylase as markers for neuroendocrine differentiation in lung cancer cell lines.
    Cancer Res. 50 (18): 6068-74.
  4. Lloyd, R.V. et al. (1984) Immunohistochemical detection of chromogranin and neuron-specific enolase in pancreatic endocrine neoplasms.
    Am J Surg Pathol. 8 (8): 607-14.
  5. Walts, A.E. et al. (1985) Chromogranin as a marker of neuroendocrine cells in cytologic material--an immunocytochemical study.
    Am J Clin Pathol. 84 (3): 273-7.
  6. Wilson, B.S. & Lloyd, R.V. (1984) Detection of chromogranin in neuroendocrine cells with a monoclonal antibody.
    Am J Pathol. 115 (3): 458-68.
  7. Wilson, B.S. et al. (1986) Chromogranin from normal human adrenal glands: purification by monoclonal antibody affinity chromatography and partial N-terminal amino acid sequence.
    Regul Pept. 13 (3-4): 207-23.
  8. Galfré, G. et al. (1977) Antibodies to major histocompatibility antigens produced by hybrid cell lines.
    Nature. 266 (5602): 550-2.
  9. Pernicová, Z. et al. (2011) Androgen depletion induces senescence in prostate cancer cells through down-regulation of Skp2.
    Neoplasia. 13: 526-36.
  10. Endo, C. et al. (2009) Immunocytochemical evaluation of large cell neuroendocrine carcinoma of the lung.
    Acta Cytol. 53: 36-40.
  11. Jeong, S.et al. (2016) Distinct metaplastic and inflammatory phenotypes in autoimmune and adenocarcinoma-associated chronic atrophic gastritis
    United European Gastroenterology Journal. Apr 11 [Epub ahead of print]
  12. Nam, K.T. et al. (2012) Gastric tumor development in Smad3-deficient mice initiates from forestomach/glandular transition zone along the lesser curvature.
    Lab Invest. 92 (6): 883-95.
  13. O'Neal, R.L. et al. (2013) Human epididymis protein 4 is up-regulated in gastric and pancreatic adenocarcinomas.
    Hum Pathol. 44 (5): 734-42.