CDw328 antibody | 5-386

Mouse anti Human CDw328

Product Type
Monoclonal Antibody
Clone
5-386
Isotype
IgG1
Specificity
CDw328

Product Code Applications Pack Size List Price Your Price Qty
MCA2522GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F 0.1 mg loader
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loader
MCA2522
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SDS Safety Datasheet SDS
C F 0.2 mg loader
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loader
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Mouse anti Human CDw328 antibody, clone 5-386 recognizes human CDw328, a type I transmembrane glycoprotein and member of the Siglec (sialic acid binding Ig-like lectin) family, designated Siglec-7, originally identified as an inhibitory NK cell receptor (NKR) and negative regulator of NK activation, attributed to ITIM recruitment of SHP-1 phosphatase.

CDw328 is expressed predominantly by natural killer cells (NK) and to a lesser extent by monocytes and granulocytes and, like Siglec-5 (CD170), has been shown to bind to sialylated ligands of targets through recognition of sialic acid in both the alpha-2,3- and alpha-2,6- glycosidic linkage.

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
Pack Size: 0.1 mg
0.09% Sodium Azide (NaN3)
Pack Size: 0.2 mg
0.09%Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Monocyte-derived dendritic cells.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Fusion Partners
Spleen cells from immunised Balb/c mice were fused with cells of the X63-Ag8.653 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/100 1/200
Immunohistology - Frozen 1/100
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Histology Positive Control Tissue
Human tonsil.

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References for CDw328 antibody

  1. Izquierdo-useros, N. et al. (2012) Siglec-1 is a novel dendritic cell receptor that mediates HIV-1 trans-infection through recognition of viral membrane gangliosides.
    PLoS Biol. 10 (12): e1001448.
  2. Perez-Zsolt, D. et al. (2019) Anti-Siglec-1 antibodies block Ebola viral uptake and decrease cytoplasmic viral entry.
    Nat Microbiol. 4 (9): 1558-70.

Further Reading

  1. Falco, M. et al. (1999) Identification and molecular cloning of p75/AIRM1, a novel member of the sialoadhesin family that functions as an inhibitory receptor in human natural killer cells.
    J Exp Med. 190 (6): 793-802.
  2. Nicoll, G. et al. (1999) Identification and characterization of a novel siglec, siglec-7, expressed by human natural killer cells and monocytes.
    J Biol Chem. 274 (48): 34089-95.

Synonyms
Siglec-7
RRID
AB_2189406
UniProt
Q9Y286
Entrez Gene
SIGLEC7
GO Terms
GO:0007155 cell adhesion
GO:0004872 receptor activity
GO:0005887 integral to plasma membrane
GO:0005529 sugar binding
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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