CD98 antibody | 44D7

Product Code Applications Pack Size List Price Your Price Qty
MCA1105G
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C F IP 0.2 mg loader
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Mouse anti Human CD98 antibody, clone 44D7 reacts with the heavy chain of the CD98 molecule. The same epitope as recognized by clone 4F2. This antibody binds to all cell lines in culture and to activated T-cells.

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
HOON pre-B leukaemia cell line.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunized BALB/c mice were fused with cells of the SP2/0-Ag14 mouse myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/50 1/200
Immunohistology - Frozen
Immunoprecipitation
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

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References for CD98 antibody

  1. Letarte, M. et al. (1986) Correlations between the 44D7 antigenic complex and the plasma membrane Na+-Ca2+ exchanger.
    Biochem Cell Biol. 64 (11): 1160-9.
  2. Jaramillo, R. et al. (2013) Relation between objective measures of atopy and myocardial infarction in the United States.
    J Allergy Clin Immunol. 131 (2): 405-11.e1-11.
  3. Månsson, A. et al. (2010) Leukocyte phenotype changes induced by specific immunotherapy in patients with birch allergy.
    J Investig Allergol Clin Immunol. 20 (6): 476-83.
  4. Millrud, C.R. et al. (2012) The activation pattern of blood leukocytes in head and neck squamous cell carcinoma is correlated to survival.
    PLoS One. 7 (12): e51120.
  5. Garrigues, H.J. et al. (2014) KSHV attachment and entry are dependent on αVβ3 integrin localized to specific cell surface microdomains and do not correlate with the presence of heparan sulfate.
    Virology. 464-465: 118-133.

Immunofluorescence

Synonyms
4F2
RRID
AB_324424
UniProt
P08195
Entrez Gene
SLC3A2
GO Terms
GO:0005515 protein binding
GO:0016049 cell growth
GO:0007596 blood coagulation
GO:0003824 catalytic activity
GO:0016021 integral to membrane
GO:0005432 calcium:sodium antiporter activity
GO:0005975 carbohydrate metabolic process
GO:0009986 cell surface
GO:0015175 neutral amino acid transmembrane transporter activity
GO:0015827 tryptophan transport
GO:0016324 apical plasma membrane
GO:0034641 cellular nitrogen compound metabolic process
GO:0042470 melanosome
GO:0043169 cation binding
GO:0050900 leukocyte migration
GO:0060356 leucine import
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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