CD95 antibody | LOB 3/17
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Mouse anti Human CD95
- Product Type
- Monoclonal Antibody
- LOB 3/17
|Mouse anti Human CD95 antibody, clone LOB 3/17 recognizes the human CD95 cell surface antigen, also known as Tumor necrosis factor receptor superfamily member 6, Fas, Apo-1 antigen, Apoptosis-mediating surface antigen FAS or FASLG receptor. CD95 is a 310 amino acid ~40-50 kDa single pass type I transmembrane glycoprotein expressed by activated T and B cells, NK cells and thymocytes. Mutations in the CD95 gene, FAS can lead to the development of Autoimmune lymphoproliferative syndrome 1A (ALPS1A), an apoptotic disorder with early onset resulting in an accumulation of autoreactive lymphocytes (Peters et al. 1999).|
- Target Species
- Species Cross-Reactivity
Target Species Cross Reactivity Rhesus Monkey
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- TRIS buffered saline
- Preservative Stabilisers
- <0.1% sodium azide (NaN3)
- Fusion protein comprising extracellular domain of human Fas linked to human Fc.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse NSI myeloma cell line.
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Frozen|
|Immunohistology - Paraffin|
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
References for CD95 antibody
Mesdaghi, M. et al. (2010) Natural killer cells in allergic rhinitis patients and nonatopic controls.
Int Arch Allergy Immunol. 153 (3): 234-8.
Ximeri, M. et al. (2010) Effect of lenalidomide therapy on hematopoiesis of patients with myelodysplastic syndrome associated with chromosome 5q deletion.
Haematologica. 95 (3): 406-14.
Aref, S. et al. (2004) Accelerated neutrophil apoptosis in neutropenic patients with hepatosplenic schistosomiasis is induced by serum Fas ligand.
Hematol J. 5 (5): 434-9.
Welsh, J.P. et al. (2004) In vitro effects of interferon-gamma and tumor necrosis factor-alpha on CD34+ bone marrow progenitor cells from aplastic anemia patients and normal donors.
Hematol J. 5 (1): 39-46.
Wethkamp, N. et al. (2011) Daxx-beta and Daxx-gamma, two novel splice variants of the transcriptional co-repressor Daxx.
J Biol Chem. 286 (22): 19576-88.
Chen, J.Y. et al. (2003) TNF-alpha renders human peritoneal mesothelial cells sensitive to anti-Fas antibody-induced apoptosis.
Nephrol Dial Transplant. 18 (9): 1741-7.
Papadaki, H.A. et al. (2002) Bone marrow progenitor cell reserve and function and stromal cell function are defective in rheumatoid arthritis: evidence for a tumor necrosis factor alpha-mediated effect.
Blood. 99 (5): 1610-9.
Mavroudi, I. et al. (2011) The CD40/CD40 ligand interactions exert pleiotropic effects on bone marrow granulopoiesis.
J Leukoc Biol. 89 (5): 771-83.
View The Latest Product References
Pyrovolaki, K. et al. (2009) Increased expression of CD40 on bone marrow CD34+ hematopoietic progenitor cells in patients with systemic lupus erythematosus: contribution to Fas-mediated apoptosis.
Arthritis Rheum. 60 (2): 543-52.
Boula, A. et al. (2006) Effect of cA2 anti-tumor necrosis factor-alpha antibody therapy on hematopoiesis of patients with myelodysplastic syndromes.
Clin Cancer Res. 12 (10): 3099-108.
Papadaki, H.A. et al. (2005) Normal bone marrow hematopoietic stem cell reserves and normal stromal cell function support the use of autologous stem cell transplantation in patients with multiple sclerosis.
Bone Marrow Transplant. 36 (12): 1053-63.
Bachsais, M. et al. (2016) The Interaction of CD154 with the α5β1 Integrin Inhibits Fas-Induced T Cell Death.
PLoS One. 11 (7): e0158987.
Ismail, M. et al. (2001) Bcl-2 and Bcl-x expression in the CD34+ cells of aplastic anaemia patients: relationship with increased apoptosis and upregulation of Fas antigen.
Br J Haematol. 113 (3): 706-12.
Bachsais, M. et al. (2020) CD154 inhibits death of T cells via a Cis interaction with the α5β1 integrin.
PLoS One. 15 (8): e0235753.
Ismail, M.M. et al. (2003) Differential apoptosis and Fas expression on GPI-negative and GPI-positive stem cells: a mechanism for the evolution of paroxysmal nocturnal haemoglobinuria.
Br J Haematol. 123 (3): 545-51.
Paulsen, M. & Janssen, O. (2011) Pro- and anti-apoptotic CD95 signaling in T cells.
Cell Commun Signal. 9: 7.
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