CD9 antibody | Bu16
Mouse anti Human CD9 antibody, clone Bu16 detects human CD9, also known as Motility-related protein-1 (MRP-1). CD9 is a 228 amino acid, including an N-terminal initiator methionine ~24-27 kDa multipass transmembrane glycoprotein and member of the tetraspanin family. It is thought that the main role of tetraspanins is to form signal transducing complexes with integrins at the cell surface, for this reason CD9 has multiple functions in cell adhesion and motility, platelet activation, gamete fusion and cell proliferation.
CD9 has a functional role as a tumour metastatic suppressor and is expressed in melanomas, and colon, lung and breast carcinomas.
CD9 has a functional role as a tumour metastatic suppressor and is expressed in melanomas, and colon, lung and breast carcinomas.
Product Details
- Target Species
- Human
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide (NaN3) 1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide (NaN3) - Carrier Free
- Yes
- Approx. Protein Concentrations
- IgG concentration 0.1mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 18 months from date of despatch.
More Information
- UniProt
- P21926 Related reagents
- Entrez Gene
- CD9 Related reagents
- GO Terms
- GO:0005515 protein binding
- GO:0002576 platelet degranulation
- GO:0007155 cell adhesion
- GO:0005887 integral to plasma membrane
- GO:0006928 cellular component movement
- GO:0007342 fusion of sperm to egg plasma membrane
- GO:0030168 platelet activation
- GO:0030913 paranodal junction assembly
- GO:0031092 platelet alpha granule membrane
- Regulatory
- For research purposes only
Applications of CD9 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat | 1/10 | |
| Flow Cytometry | Neat | 1/200 | |
| Immunofluorescence | |||
| Immunoprecipitation | |||
| Western Blotting |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
- Western Blotting
- MCA2655 detects a band of approximately 25kDa in HeLa cell lysates.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG2a Negative Control:FITC | MCA929F | 100 Tests | F | ||
| Mouse IgG2a Negative Control | MCA929 | 100 Tests | F |
Useful Reagents Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Human Seroblock | BUF070A | 50 Test | F | ||
| Human Seroblock | BUF070B | 200 Test | F |
Product Specific References
Further Reading
-
Huang, C.L. et al. (2004) MRP-1/CD9 gene transduction downregulates Wnt signal pathways.
Oncogene. 23 (45): 7475-83. -
Huang, C.L. et al. (2006) MRP-1/CD9 gene transduction regulates the actin cytoskeleton through the downregulation of WAVE2.
Oncogene. 25 (49): 6480-8.
