Mouse anti Human CD9 antibody, clone Bu16 detects human CD9, also known as Motility-related protein-1 (MRP-1). CD9 is a 228 amino acid, including an N-terminal initiator methionine ~24-27 kDa multipass transmembrane glycoprotein and member of the tetraspanin family. It is thought that the main role of tetraspanins is to form signal transducing complexes with integrins at the cell surface, for this reason CD9 has multiple functions in cell adhesion and motility, platelet activation, gamete fusion and cell proliferation.
CD9 has a functional role as a tumour metastatic suppressor and is expressed in melanomas, and colon, lung and breast carcinomas.
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- Carrier Free
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
integral to plasma membrane
cellular component movement
fusion of sperm to egg plasma membrane
paranodal junction assembly
platelet alpha granule membrane
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of CD9 antibody
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
- Western Blotting
- MCA2655 detects a band of approximately 25kDa in HeLa cell lysates.
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Secondary Antibodies Available
Negative Isotype Controls Available
Product Specific References
Huang, C.L. et al. (2004) MRP-1/CD9 gene transduction downregulates Wnt signal pathways.
Oncogene. 23 (45): 7475-83.
Huang, C.L. et al. (2006) MRP-1/CD9 gene transduction regulates the actin cytoskeleton through the downregulation of WAVE2.
Oncogene. 25 (49): 6480-8.
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