CD89 antibody | MIP8a
Mouse anti Human CD89 antibody, clone MIP8a (MCA1824) used to inhibit phagocytosis by human neutrophils in vitro
Image caption:
MIP8a FcαRI blocking antibodies do not stimulate Fc-receptor inhibitory pathways. (A) Human neutrophils were incubated with fluorescent BSA- (white bar) or IgA-coated (red bars) beads. Quantification of phagocytosis is depicted in the presence of anti-FcαRI MIP8a blocking antibodies (whole IgG) or as F(ab′)2. Data of three donors are presented as mean ± SD. Student’s t test; **p <0.01.
From: Bos A, Aleyd E, van der Steen LPE, Winter PJ, Heemskerk N, Pouw SM, Boon L, Musters RJP, Bakema JE, Sitaru C, Cogné M, van Egmond M.
Anti-FcαRI Monoclonal Antibodies Resolve IgA Autoantibody-Mediated Disease.
Front Immunol. 2022 Mar 15;13:732977.
doi: 10.3389/fimmu.2022.732977.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human CD89
- Product Type
- Monoclonal Antibody
- Clone
- MIP8a
- Isotype
- IgG1
- Specificity
- CD89
Antibody Quality and Performance Guarantee
| Mouse anti Human CD89 antibody, clone MIP8a recognizes the human CD89 cell surface antigen, a 50-75 kDa cell surface glycoprotein that is also known as the IgA receptor (Fc alpha R). CD89 is expressed by peripheral blood monocytes and neutrophils. MIP8a blocks binding of IgA to the Fc alpha R, and also inhibits neutrophil phagocytosis of IgA complexes. |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- MCA1824: Purified IgG from tissue culture supernatant
- MCA1824GA: Antibody purified from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Immunogen
- Recombinant soluble human Fc alpha R.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Pack Size: 0.2 mg
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Pack Size: 0.1 mg
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
||
| Flow Cytometry | |
1/50 | 1/100 |
| Immunoprecipitation | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells or 100μl whole blood
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
|
Log in | ||
| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control | ||||||
References for CD89 antibody
-
Zhang, W. et al. (2000) Neutrophil lactoferrin release induced by IgA immune complexes differed from that induced by cross-linking of fcalpha receptors (FcalphaR) with a monoclonal antibody, MIP8a.
Clin Exp Immunol. 121 (1): 106-11. -
Lu, J. et al. (2011) Recognition and functional activation of the human IgA receptor (Fc{alpha}RI) by C-reactive protein.
Proc Natl Acad Sci U S A. 108: 4974-9. -
Duc, M. et al. (2010) Antigen binding to secretory immunoglobulin A results in decreased sensitivity to intestinal proteases and increased binding to cellular Fc receptors.
J Biol Chem. 285: 953-60. -
Wu, J. et al. (2007) FcαRI (CD89) alleles determine the proinflammatory potential of serum IgA.
J Immunol. 178: 3973-82. -
Hamre, R. et al. (2003) Expression and modulation of the human immunoglobulin A Fc receptor (CD89) and the FcR gamma chain on myeloid cells in blood and tissue.
Scand J Immunol. 57: 506-16. -
Qian,K. et al. (2008) Functional expression of IgA receptor FcalphaRI on human platelets.
J Leukoc Biol. 84: 1492-500. -
Van Egmond, M. (2011) Method for the treatment or prophylaxis of chronic inflammatory diseases.
European Patent Application No: 12/736963 -
Pascal, V. et al. (2012) Anti-CD20 IgA can protect mice against lymphoma development: evaluation of the direct impact of IgA and cytotoxic effector recruitment on CD20 target cells.
Haematologica. 97 (11): 1686-94.
View The Latest Product References
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Mladenov, R. et al. (2015) The Fc-alpha receptor is a new target antigen for immunotherapy of myeloid leukemia.
Int J Cancer. 137 (11): 2729-38. -
Aleyd, E. et al. (2016) IgA Complexes in Plasma and Synovial Fluid of Patients with Rheumatoid Arthritis Induce Neutrophil Extracellular Traps via FcαRI.
J Immunol. 197 (12): 4552-9. -
van der Steen, L. et al. (2009) Immunoglobulin A: Fc(alpha)RI interactions induce neutrophil migration through release of leukotriene B4.
Gastroenterology. 137 (6): 2018-29.e1-3. -
Hamre, R. et al. (2003) Expression and modulation of the human immunoglobulin A Fc receptor (CD89) and the FcR gamma chain on myeloid cells in blood and tissue.
Scand J Immunol. 57 (6): 506-16. -
Lu, J. et al. (2014) Pentraxins and IgA share a binding hot-spot on FcαRI.
Protein Sci. 23 (4): 378-86. -
Askarian, F. et al. (2021) The lytic polysaccharide monooxygenase CbpD promotes Pseudomonas aeruginosa. virulence in systemic infection.
Nat Commun. 12 (1): 1230. -
Bos, A. et al. (2022) Anti-FcαRI Monoclonal Antibodies Resolve IgA Autoantibody-Mediated Disease.
Front Immunol. 13: 732977.
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