CD88 antibody | S5/1

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Anti Human CD88 Antibody, clone S5/1 thumbnail image 1

Anti Human CD88 Antibody, clone S5/1 thumbnail image 2

Anti Human CD88 Antibody, clone S5/1 thumbnail image 3

Anti Human CD88 Antibody, clone S5/1 thumbnail image 4

Anti Human CD88 Antibody, clone S5/1 gallery image 1 — Staining of human peripheral blood granulocytes with Mouse anti Human CD88 antibody, clone S5/1 (**MCA1283**).

Anti Human CD88 Antibody, clone S5/1 gallery image 2 — Staining of paraffin embedded human lung with Mouse anti Human CD88 antibody, clone S5/1 (**MCA1283**).

Anti Human CD88 Antibody, clone S5/1 gallery image 3 — Staining of human peripheral blood granulocytes with Low endotoxin Mouse anti Human CD88 antibody, clone S5/1 (**MCA1283EL**) followed by Rabbit F(ab')₂ anti Mouse IgG:FITC (STAR9B).

Anti Human CD88 Antibody, clone S5/1 gallery image 4 — FFPE human spleen stained with Mouse Anti-Human CD88 antibody, clone S5/1(MCA1283), biotinylated Anti-Mouse IgG and Streptavidin Alkaline Phosphatase (red). Nuclei were counterstained with hematoxylin.

Mouse anti Human CD88:FITC

Mouse anti Human CD88:Low Endotoxin

Mouse anti Human CD88

Mouse anti Human CD88:RPE

Product Type: Monoclonal Antibody
Clone: S5/1
Isotype: IgG2a

Product Code	Applications	Pack Size
MCA1283F	F	0.1 mg
MCA1283EL	F FN P	0.5 mg
MCA1283T	F FN* P WB	25 µg
MCA1283	F FN* P WB	0.2 mg
MCA1283PE	F	100 Tests

Mouse anti Human CD88 antibody, clone S5/1 recognizes the C5a receptor (C5aR) CD88, which is predominantly expressed on cells of the myeloid lineage. Clone S5/1 was raised against a synthetic peptide comprising the N-terminal extracellular domain of the C5aR (met1-Asn31) and has recently been shown to recognise the heptameric peptide (D15DKDTLD21).

Clone S5/1 has been shown to inhibit the binding of C5a to its receptor.

Product Details

Target Species

Human

Species Cross-Reactivity

Target Species	Cross Reactivity
Rabbit
Mustelid	Expected from Sequence
Bovine
Ferret
Mink

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid

Product Form

Purified IgG - liquid

Product Form

Pack Size: 25 µg

Ig fraction - liquid

Pack Size: 0.2 mg

Purified IgG - liquid

Product Form

Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized

Reconstitution

Reconstitute with 1.0 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.

Preparation

Purified IgG prepared by affinity chromatography on Protein A

Preparation

Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant

Preparation

Purified IgG prepared by affinity chromatography on Protein G

Preparation

Purified IgG prepared by affinity chromatography on Protein A

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin

Preservative Stabilisers

None present

Preservative Stabilisers

Pack Size: 25 µg

0.09%

Sodium Azide

Pack Size: 0.2 mg

0.09% Sodium Azide (NaN₃)

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
5% Sucrose

Carrier Free

Yes

Carrier Free

Yes

Immunogen

C5aR - peptide: Met1 - Asn31.

Approx. Protein Concentrations

IgG concentration 0.1 mg/ml

Approx. Protein Concentrations

IgG concentration 1 mg/ml

Approx. Protein Concentrations

Pack Size: 25 µg

Ig concentration 1 mg/ml

Pack Size: 0.2 mg

IgG concentration 1.0 mg/ml

Fusion Partners

Spleen cells from immunised BALB/c mice were fused with cells of the X63-Ag8 myeloma cell line.

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at -20^oC only.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC or at -20^oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Prior to reconstitution store at +4^oC.
After reconstitution store at +4^oC.
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light.
Guarantee: 18 months from date of despatch
Guarantee: 18 months from date of despatch.
Guarantee: 18 months from date of despatch.
Guarantee: 12 months from date of reconstitution

More Information

UniProt: P21730 Related reagents
Entrez Gene: C5AR1 Related reagents
GO Terms: GO:0000187 activation of MAPK activity; GO:0004944 C5a anaphylatoxin receptor activity; GO:0005887 integral to plasma membrane; GO:0006955 immune response; GO:0006968 cellular defense response; GO:0007202 activation of phospholipase C activity; GO:0007204 elevation of cytosolic calcium ion concentration; GO:0007606 sensory perception of chemical stimulus
Regulatory: For research purposes only

Applications of CD88 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
Flow Cytometry	Neat
Flow Cytometry	1/25	1/200
Functional Assays
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Flow Cytometry	1/50	1/100
Functional Assays ¹
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
Flow Cytometry	Neat

¹ Bio-Rad recommend the use of MCA1283EL for functional studies.

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 10ul of the suggested working dilution to label 1x10⁶ cells in 100ul.
Flow Cytometry: Use 10ul of the suggested working dilution to label 5 x 10⁵ cells in 100ul.
Flow Cytometry: Use 10ul of the suggested working dilution to label 5 x 10⁵ cells in 100ul.
Flow Cytometry: Use 10ul of the suggested working dilution to label 1x10⁶ cells in 100ul
Immunohistology: This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Human anti Mouse IgG2a:FITC	HCA037F	0.1 mg	F
Human anti Mouse IgG2a:HRP	HCA037P	0.1 mg	E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F
Human anti Mouse IgG2a:FITC	HCA037F	0.1 mg	F
Human anti Mouse IgG2a:HRP	HCA037P	0.1 mg	E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Negative Isotype Controls Available

Description	Product Code	Pack Size	Applications
Mouse IgG2a Negative Control:FITC	MCA929F	100 Tests	F
Mouse IgG2a Negative Control:Low Endotoxin	MCA929EL	0.5 mg	F
Mouse IgG2a Negative Control	MCA929	100 Tests	F
Mouse IgG2a Negative Control:RPE	MCA929PE	100 Tests	F

Useful Reagents Available

Description	Product Code	Pack Size	Applications
Human Seroblock	BUF070A	50 Test	F
Human Seroblock	BUF070B	200 Test	F
Human Seroblock	BUF070A	50 Test	F
Human Seroblock	BUF070B	200 Test	F

Application Based External Images

Flow Cytometry

Functional Assays

Immunoprecipitation

Radioimmunoassays

Product Specific References

References for CD88 antibody

Oppermann, M. et al. (1993) Probing the human receptor for C5a anaphylatoxin with site-directed antibodies. Identification of a potential ligand binding site on the NH2-terminal domain.
J Immunol. 151: 3785-94.
Oppermann, M. et al. (1995) Antibodies from the myeloid panel that react with the C5a receptor and antagonize C5a biological activity.
In: Schlossman, S.F. (ed.) Leucocyte Typing V. O.U.P. pp 955-956.
Werfel, T. et al. (1996) CD88 antibodies specifically bind to C5aR on dermal CD117+ and CD14+ cells and react with a desmosomal antigen in human skin.
J Immunol. 157 (4): 1729-35.
Oppermann, M. & Götze, O. (1994) Plasma clearance of the human C5a anaphylatoxin by binding to leucocyte C5a receptors.
Immunology. 82 (4): 516-21.
Martel, C.J. & Aasted, B. (2009) Characterization of antibodies against ferret immunoglobulins, cytokines and CD markers.
Vet Immunol Immunopathol. 132:109-15.
Sopp, P. et al. (2007) Cross-reactivity of mAbs to human CD antigens with cells from cattle.
Vet Immunol Immunopathol. 119: 106-14.
Camous, L. et al. (2011) Complement alternative pathway acts as a positive feedback amplification of neutrophil activation.
Blood. 117: 1340-9.
Corrales, L. et al. (2012) Anaphylatoxin C5a Creates a Favorable Microenvironment for Lung Cancer Progression.
J Immunol. 189: 4674-83.
Hüttenrauch, F. et al. (2005) G protein-coupled receptor kinases promote phosphorylation and beta-arrestin-mediated internalization of CCR5 homo- and hetero-oligomers.
J Biol Chem. 280: 37503-15.
Sumichika, H. et al. (2002) Identification of a potent and orally active non-peptide C5a receptor antagonist.
J Biol Chem. 277: 49403-7.
Thivierge, M. et al. (1999) Modulation of formyl peptide receptor expression by IL-10 in human monocytes and neutrophils.
J Immunol. 162: 3590-5.
Schreiber, A. et al. (2009) C5a receptor mediates neutrophil activation and ANCA-induced glomerulonephritis.
J Am Soc Nephrol. 20: 289-98.
Eglite, S. et al. (2000) Requirements for C5a receptor-mediated IL-4 and IL-13 production and leukotriene C4 generation in human basophils.
J Immunol. 165: 2183-9.
Conroy, A. et al. (2009) C5a enhances dysregulated inflammatory and angiogenic responses to malaria in vitro: potential implications for placental malaria.
PLoS One. 4: e4953.
Kraft, K. et al. (2001) Characterization of sequence determinants within the carboxyl-terminal domain of chemokine receptor CCR5 that regulate signaling and receptor internalization.
J Biol Chem. 276: 34408-18.
Fukuoka, Y. et al. (2008) Generation of anaphylatoxins by human beta-tryptase from C3, C4, and C5.
J Immunol. 180: 6307-16.
Aasted, B. and Viuff, B. (2007) Reactivity of monoclonal antibodies to human CD antigens with cells from mink.
Vet Immunol Immunopathol. 119: 27-37.
Huang, L. et al. (2005) Discovery of human antibodies against the C5aR target using phage display technology.
J Mol Recognit.18: 327-33.
Tseng CW et al. (2015) Increased Susceptibility of Humanized NSG Mice to Panton-Valentine Leukocidin and Staphylococcus aureus Skin Infection.
PLoS Pathog. 11 (11): e1005292.
Bettoni, S. et al. (2017) Interaction between Multimeric von Willebrand Factor and Complement: A Fresh Look to the Pathophysiology of Microvascular Thrombosis.
J Immunol. 199 (3): 1021-40.

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