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CD88 antibody | S5/1

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Anti Human CD88 Antibody, clone S5/1 thumbnail image 1 Anti Human CD88 Antibody, clone S5/1 thumbnail image 2 Anti Human CD88 Antibody, clone S5/1 thumbnail image 3 Anti Human CD88 Antibody, clone S5/1 thumbnail image 4
Anti Human CD88 Antibody, clone S5/1 gallery image 1
Staining of human peripheral blood granulocytes with Mouse anti Human CD88 antibody, clone S5/1 (MCA1283).
Anti Human CD88 Antibody, clone S5/1 gallery image 2
Staining of paraffin embedded human lung with Mouse anti Human CD88 antibody, clone S5/1 (MCA1283).
Anti Human CD88 Antibody, clone S5/1 gallery image 3
Staining of human peripheral blood granulocytes with Low endotoxin Mouse anti Human CD88 antibody, clone S5/1 (MCA1283EL) followed by Rabbit F(ab')2 anti Mouse IgG:FITC (STAR9B).
Anti Human CD88 Antibody, clone S5/1 gallery image 4
Mouse anti Human CD88 antibody, clone S5/1(MCA1283) used to stain formalin fixed, paraffin embedded human spleen sections followed by biotinylated anti Mouse IgG and streptavidin alkaline phosphatase (red). Nuclei were counterstained with hematoxylin (blue).

Mouse anti Human CD88:FITC

Mouse anti Human CD88:Low Endotoxin

Mouse anti Human CD88

Mouse anti Human CD88:RPE

Product Type
Monoclonal Antibody
Clone
S5/1
Isotype
IgG2a
Product Code Applications Pack Size List Price Quantity
MCA1283F
Datasheet
F
SDS
0.1 mg loader

MCA1283EL
Datasheet
F FN P
SDS
0.5 mg loader

MCA1283
Datasheet
F FN* P WB
SDS
0.2 mg loader

MCA1283T
Datasheet
F FN* P WB
SDS
25 µg loader

MCA1283PE
Datasheet
F
SDS
100 Tests loader

Mouse anti Human CD88 antibody, clone S5/1 recognizes the C5a receptor (C5aR) CD88, which is predominantly expressed on cells of the myeloid lineage. Clone S5/1 was raised against a synthetic peptide comprising the N-terminal extracellular domain of the C5aR (met1-Asn31) and has recently been shown to recognise the heptameric peptide (D15DKDTLD21).

Clone S5/1 has been shown to inhibit the binding of C5a to its receptor.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rabbit
Mustelid Expected from Sequence
Bovine
Ferret
Mink
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1.0 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preservative Stabilisers
None present
Preservative Stabilisers
Pack Size: 0.2 mg
0.09% Sodium Azide (NaN3)
Pack Size: 25 µg
0.09%Sodium Azide
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose
Carrier Free
Yes
Carrier Free
Yes
Immunogen
C5aR - peptide: Met1 - Asn31.
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Approx. Protein Concentrations
IgG concentration 1 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the X63-Ag8 myeloma cell line.

Storage Information

Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Storage
Store at -20oC only.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Storage
Prior to reconstitution store at +4oC.
After reconstitution store at +4oC.
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light.
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
12 months from date of despatch

More Information

UniProt
P21730
Entrez Gene
C5AR1
GO Terms
GO:0000187 activation of MAPK activity
GO:0004944 C5a anaphylatoxin receptor activity
GO:0005887 integral to plasma membrane
GO:0006955 immune response
GO:0006968 cellular defense response
GO:0007202 activation of phospholipase C activity
GO:0007204 elevation of cytosolic calcium ion concentration
GO:0007606 sensory perception of chemical stimulus
Regulatory
For research purposes only

Applications of CD88 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Flow Cytometry 1/25 1/200
Functional Assays
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Flow Cytometry 1/50 1/100
Functional Assays 1
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
Flow Cytometry Neat
  1. 1 Bio-Rad recommend the use of MCA1283EL for functional studies.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 5 x 105 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 5 x 105 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
Immunohistology
This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Human anti Mouse IgG2a:HRP HCA037P E 0.1 mg loader
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader
Human anti Mouse IgG2a:HRP HCA037P E 0.1 mg loader
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P C E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Mouse IgG2a Negative Control:FITC MCA929F F 100 Tests loader
Mouse IgG2a Negative Control:Low Endotoxin MCA929EL F 0.5 mg loader
Mouse IgG2a Negative Control MCA929 F 100 Tests loader
Mouse IgG2a Negative Control:RPE MCA929PE F 100 Tests loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Human Seroblock BUF070A F 50 Test
Human Seroblock BUF070B F 200 Test
Human Seroblock BUF070A F 50 Test
Human Seroblock BUF070B F 200 Test

Application Based External Images

Flow Cytometry

Functional Assays

Immunoprecipitation

Radioimmunoassays

Product Specific References

Source Reference

  1. Oppermann, M. et al. (1993) Probing the human receptor for C5a anaphylatoxin with site-directed antibodies. Identification of a potential ligand binding site on the NH2-terminal domain.
    J Immunol. 151: 3785-94.

References for CD88 antibody

  1. Oppermann, M. et al. (1995) Antibodies from the myeloid panel that react with the C5a receptor and antagonize C5a biological activity.
    In: Schlossman, S.F. (ed.) Leucocyte Typing V. O.U.P. pp 955-956.
  2. Oppermann, M. & Götze, O. (1994) Plasma clearance of the human C5a anaphylatoxin by binding to leucocyte C5a receptors.
    Immunology. 82 (4): 516-21.
  3. Werfel, T. et al. (1996) CD88 antibodies specifically bind to C5aR on dermal CD117+ and CD14+ cells and react with a desmosomal antigen in human skin.
    J Immunol. 157 (4): 1729-35.
  4. Sumichika, H. et al. (2002) Identification of a potent and orally active non-peptide C5a receptor antagonist.
    J Biol Chem. 277: 49403-7.
  5. Martel, C.J. & Aasted, B. (2009) Characterization of antibodies against ferret immunoglobulins, cytokines and CD markers.
    Vet Immunol Immunopathol. 132:109-15.
  6. Sopp, P. et al. (2007) Cross-reactivity of mAbs to human CD antigens with cells from cattle.
    Vet Immunol Immunopathol. 119: 106-14.
  7. Camous, L. et al. (2011) Complement alternative pathway acts as a positive feedback amplification of neutrophil activation.
    Blood. 117: 1340-9.
  8. Corrales, L. et al. (2012) Anaphylatoxin C5a Creates a Favorable Microenvironment for Lung Cancer Progression.
    J Immunol. 189: 4674-83.
  9. Hüttenrauch, F. et al. (2005) G protein-coupled receptor kinases promote phosphorylation and beta-arrestin-mediated internalization of CCR5 homo- and hetero-oligomers.
    J Biol Chem. 280: 37503-15.
  10. Aasted, B. and Viuff, B. (2007) Reactivity of monoclonal antibodies to human CD antigens with cells from mink.
    Vet Immunol Immunopathol. 119: 27-37.
  11. Thivierge, M. et al. (1999) Modulation of formyl peptide receptor expression by IL-10 in human monocytes and neutrophils.
    J Immunol. 162: 3590-5.
  12. Schreiber, A. et al. (2009) C5a receptor mediates neutrophil activation and ANCA-induced glomerulonephritis.
    J Am Soc Nephrol. 20: 289-98.
  13. Eglite, S. et al. (2000) Requirements for C5a receptor-mediated IL-4 and IL-13 production and leukotriene C4 generation in human basophils.
    J Immunol. 165: 2183-9.
  14. Conroy, A. et al. (2009) C5a enhances dysregulated inflammatory and angiogenic responses to malaria in vitro: potential implications for placental malaria.
    PLoS One. 4: e4953.
  15. Kraft, K. et al. (2001) Characterization of sequence determinants within the carboxyl-terminal domain of chemokine receptor CCR5 that regulate signaling and receptor internalization.
    J Biol Chem. 276: 34408-18.
  16. Fukuoka, Y. et al. (2008) Generation of anaphylatoxins by human beta-tryptase from C3, C4, and C5.
    J Immunol. 180: 6307-16.
  17. Bettoni, S. et al. (2017) Interaction between Multimeric von Willebrand Factor and Complement: A Fresh Look to the Pathophysiology of Microvascular Thrombosis.
    J Immunol. 199 (3): 1021-40.
  18. Huang, L. et al. (2005) Discovery of human antibodies against the C5aR target using phage display technology.
    J Mol Recognit.18: 327-33.
  19. Tseng CW et al. (2015) Increased Susceptibility of Humanized NSG Mice to Panton-Valentine Leukocidin and Staphylococcus aureus Skin Infection.
    PLoS Pathog. 11 (11): e1005292.

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