CD88 antibody | S5/1




Mouse anti Human CD88:FITC
Mouse anti Human CD88:Low Endotoxin
Mouse anti Human CD88
Mouse anti Human CD88:RPE
- Product Type
- Monoclonal Antibody
- Clone
- S5/1
- Isotype
- IgG2a
Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|
MCA1283F | F | 0.1 mg |
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MCA1283EL | F FN P | 0.5 mg |
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MCA1283T | F FN* P WB | 25 µg |
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MCA1283 | F FN* P WB | 0.2 mg |
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MCA1283PE | F | 100 Tests |
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Clone S5/1 has been shown to inhibit the binding of C5a to its receptor.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rabbit Mustelid Expected from Sequence Bovine Ferret Mink - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Reconstitution
- Reconstitute with 1.0 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. - Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Preservative Stabilisers
- None present
- Preservative Stabilisers
- Pack Size: 25 µg
0.09% Sodium Azide Pack Size: 0.2 mg0.09% Sodium Azide (NaN3) - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose - Carrier Free
- Yes
- Carrier Free
- Yes
- Immunogen
- C5aR - peptide: Met1 - Asn31.
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the X63-Ag8 myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at -20oC only.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Prior to reconstitution store at +4oC.
After reconstitution store at +4oC.
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P21730
- Entrez Gene
- C5AR1
- GO Terms
- GO:0000187 activation of MAPK activity
- GO:0004944 C5a anaphylatoxin receptor activity
- GO:0005887 integral to plasma membrane
- GO:0006955 immune response
- GO:0006968 cellular defense response
- GO:0007202 activation of phospholipase C activity
- GO:0007204 elevation of cytosolic calcium ion concentration
- GO:0007606 sensory perception of chemical stimulus
- Regulatory
- For research purposes only
Applications of CD88 antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | Neat | ||
Flow Cytometry | 1/25 | 1/200 | |
Functional Assays | |||
Immunohistology - Frozen | |||
Immunohistology - Paraffin | |||
Immunoprecipitation | |||
Flow Cytometry | 1/50 | 1/100 | |
Functional Assays 1 | |||
Immunohistology - Frozen | |||
Immunohistology - Paraffin | |||
Immunoprecipitation | |||
Western Blotting | |||
Flow Cytometry | Neat |
- 1 Bio-Rad recommend the use of MCA1283EL for functional studies.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 5 x 105 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 5 x 105 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
- Immunohistology
- This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
Secondary Antibodies Available
Negative Isotype Controls Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Mouse IgG2a Negative Control:FITC | MCA929F | F | 100 Tests |
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Mouse IgG2a Negative Control:Low Endotoxin | MCA929EL | F | 0.5 mg |
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Mouse IgG2a Negative Control | MCA929 | F | 100 Tests |
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Mouse IgG2a Negative Control:RPE | MCA929PE | F | 100 Tests |
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Useful Reagents Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Human Seroblock | BUF070A | F | 50 Test | ||
Human Seroblock | BUF070B | F | 200 Test | ||
Human Seroblock | BUF070A | F | 50 Test | ||
Human Seroblock | BUF070B | F | 200 Test |
Application Based External Images
Flow Cytometry
Functional Assays
Immunoprecipitation
Radioimmunoassays
Product Specific References
Source Reference
-
Oppermann, M. et al. (1993) Probing the human receptor for C5a anaphylatoxin with site-directed antibodies. Identification of a potential ligand binding site on the NH2-terminal domain.
J Immunol. 151: 3785-94.
References for CD88 antibody
-
Oppermann, M. et al. (1995) Antibodies from the myeloid panel that react with the C5a receptor and antagonize C5a biological activity.
In: Schlossman, S.F. (ed.) Leucocyte Typing V. O.U.P. pp 955-956. -
Oppermann, M. & Götze, O. (1994) Plasma clearance of the human C5a anaphylatoxin by binding to leucocyte C5a receptors.
Immunology. 82 (4): 516-21. -
Werfel, T. et al. (1996) CD88 antibodies specifically bind to C5aR on dermal CD117+ and CD14+ cells and react with a desmosomal antigen in human skin.
J Immunol. 157 (4): 1729-35. -
Sumichika, H. et al. (2002) Identification of a potent and orally active non-peptide C5a receptor antagonist.
J Biol Chem. 277: 49403-7. -
Martel, C.J. & Aasted, B. (2009) Characterization of antibodies against ferret immunoglobulins, cytokines and CD markers.
Vet Immunol Immunopathol. 132:109-15. -
Sopp, P. et al. (2007) Cross-reactivity of mAbs to human CD antigens with cells from cattle.
Vet Immunol Immunopathol. 119: 106-14. -
Camous, L. et al. (2011) Complement alternative pathway acts as a positive feedback amplification of neutrophil activation.
Blood. 117: 1340-9. -
Corrales, L. et al. (2012) Anaphylatoxin C5a Creates a Favorable Microenvironment for Lung Cancer Progression.
J Immunol. 189: 4674-83. -
Hüttenrauch, F. et al. (2005) G protein-coupled receptor kinases promote phosphorylation and beta-arrestin-mediated internalization of CCR5 homo- and hetero-oligomers.
J Biol Chem. 280: 37503-15. -
Aasted, B. and Viuff, B. (2007) Reactivity of monoclonal antibodies to human CD antigens with cells from mink.
Vet Immunol Immunopathol. 119: 27-37. -
Thivierge, M. et al. (1999) Modulation of formyl peptide receptor expression by IL-10 in human monocytes and neutrophils.
J Immunol. 162: 3590-5. -
Schreiber, A. et al. (2009) C5a receptor mediates neutrophil activation and ANCA-induced glomerulonephritis.
J Am Soc Nephrol. 20: 289-98. -
Eglite, S. et al. (2000) Requirements for C5a receptor-mediated IL-4 and IL-13 production and leukotriene C4 generation in human basophils.
J Immunol. 165: 2183-9. -
Conroy, A. et al. (2009) C5a enhances dysregulated inflammatory and angiogenic responses to malaria in vitro: potential implications for placental malaria.
PLoS One. 4: e4953. -
Kraft, K. et al. (2001) Characterization of sequence determinants within the carboxyl-terminal domain of chemokine receptor CCR5 that regulate signaling and receptor internalization.
J Biol Chem. 276: 34408-18. -
Fukuoka, Y. et al. (2008) Generation of anaphylatoxins by human beta-tryptase from C3, C4, and C5.
J Immunol. 180: 6307-16. -
Bettoni, S. et al. (2017) Interaction between Multimeric von Willebrand Factor and Complement: A Fresh Look to the Pathophysiology of Microvascular Thrombosis.
J Immunol. 199 (3): 1021-40. -
Huang, L. et al. (2005) Discovery of human antibodies against the C5aR target using phage display technology.
J Mol Recognit.18: 327-33. -
Tseng CW et al. (2015) Increased Susceptibility of Humanized NSG Mice to Panton-Valentine Leukocidin and Staphylococcus aureus Skin Infection.
PLoS Pathog. 11 (11): e1005292.
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