CD88 antibody | S5/1
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F P ResetMouse anti Human CD88
- Product Type
- Monoclonal Antibody
- Clone
- S5/1
- Isotype
- IgG2a
- Specificity
- CD88
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Antibody Quality and 
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| Mouse anti Human CD88 antibody, clone S5/1 recognizes the C5a receptor (C5aR) CD88, which is predominantly expressed on cells of the myeloid lineage. Clone S5/1 was raised against a synthetic peptide comprising the N-terminal extracellular domain of the C5aR (met1-Asn31) and has recently been shown to recognise the heptameric peptide (D15DKDTLD21). Clone S5/1 has been shown to inhibit the binding of C5a to its receptor. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rabbit Mustelid Expected from Sequence Bovine Ferret Mink - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- Pack Size: 0.2 mg0.09% Sodium Azide (NaN3)Pack Size: 25 µg
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- C5aR - peptide: Met1 - Asn31.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the X63-Ag8 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/50 | 1/100 |
| Functional Assays 1 | |
||
| Immunohistology - Frozen | |||
| Immunohistology - Paraffin | |
||
| Immunoprecipitation | |||
| Western Blotting | |
- 1 Bio-Rad recommend the use of MCA1283EL for functional studies.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 5 x 105 cells in 100ul.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG2a Negative Control | MCA929 | F | 100 Tests |
|
Log in | ||
| List Price | Your Price | ||||||
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| Description | Mouse IgG2a Negative Control | ||||||
Source Reference
-
Oppermann, M. et al. (1993) Probing the human receptor for C5a anaphylatoxin with site-directed antibodies. Identification of a potential ligand binding site on the NH2-terminal domain.
J Immunol. 151: 3785-94.
References for CD88 antibody
-
Oppermann, M. et al. (1995) Antibodies from the myeloid panel that react with the C5a receptor and antagonize C5a biological activity.
In: Schlossman, S.F. (ed.) Leucocyte Typing V. O.U.P. pp 955-956. -
Oppermann, M. & Götze, O. (1994) Plasma clearance of the human C5a anaphylatoxin by binding to leucocyte C5a receptors.
Immunology. 82 (4): 516-21. -
Werfel, T. et al. (1996) CD88 antibodies specifically bind to C5aR on dermal CD117+ and CD14+ cells and react with a desmosomal antigen in human skin.
J Immunol. 157 (4): 1729-35. -
Sumichika, H. et al. (2002) Identification of a potent and orally active non-peptide C5a receptor antagonist.
J Biol Chem. 277: 49403-7. -
Martel, C.J. & Aasted, B. (2009) Characterization of antibodies against ferret immunoglobulins, cytokines and CD markers.
Vet Immunol Immunopathol. 132:109-15. -
Sopp, P. et al. (2007) Cross-reactivity of mAbs to human CD antigens with cells from cattle.
Vet Immunol Immunopathol. 119: 106-14. -
Camous, L. et al. (2011) Complement alternative pathway acts as a positive feedback amplification of neutrophil activation.
Blood. 117: 1340-9. -
Corrales, L. et al. (2012) Anaphylatoxin C5a Creates a Favorable Microenvironment for Lung Cancer Progression.
J Immunol. 189: 4674-83.
View The Latest Product References
-
Hüttenrauch, F. et al. (2005) G protein-coupled receptor kinases promote phosphorylation and beta-arrestin-mediated internalization of CCR5 homo- and hetero-oligomers.
J Biol Chem. 280: 37503-15. -
Aasted, B. and Viuff, B. (2007) Reactivity of monoclonal antibodies to human CD antigens with cells from mink.
Vet Immunol Immunopathol. 119: 27-37. -
Thivierge, M. et al. (1999) Modulation of formyl peptide receptor expression by IL-10 in human monocytes and neutrophils.
J Immunol. 162: 3590-5. -
Schreiber, A. et al. (2009) C5a receptor mediates neutrophil activation and ANCA-induced glomerulonephritis.
J Am Soc Nephrol. 20: 289-98. -
Eglite, S. et al. (2000) Requirements for C5a receptor-mediated IL-4 and IL-13 production and leukotriene C4 generation in human basophils.
J Immunol. 165: 2183-9. -
Conroy, A. et al. (2009) C5a enhances dysregulated inflammatory and angiogenic responses to malaria in vitro: potential implications for placental malaria.
PLoS One. 4: e4953. -
Kraft, K. et al. (2001) Characterization of sequence determinants within the carboxyl-terminal domain of chemokine receptor CCR5 that regulate signaling and receptor internalization.
J Biol Chem. 276: 34408-18. -
Fukuoka, Y. et al. (2008) Generation of anaphylatoxins by human beta-tryptase from C3, C4, and C5.
J Immunol. 180: 6307-16. -
Bettoni, S. et al. (2017) Interaction between Multimeric von Willebrand Factor and Complement: A Fresh Look to the Pathophysiology of Microvascular Thrombosis.
J Immunol. 199 (3): 1021-40. -
Huang, L. et al. (2005) Discovery of human antibodies against the C5aR target using phage display technology.
J Mol Recognit.18: 327-33. -
Tseng CW et al. (2015) Increased Susceptibility of Humanized NSG Mice to Panton-Valentine Leukocidin and Staphylococcus aureus Skin Infection.
PLoS Pathog. 11 (11): e1005292.
- Synonyms
- C5aR
- RRID
- AB_321883
- UniProt
- P21730
- Entrez Gene
- C5AR1
- GO Terms
- GO:0000187 activation of MAPK activity
- GO:0004944 C5a anaphylatoxin receptor activity
- GO:0005887 integral to plasma membrane
- GO:0006955 immune response
- GO:0006968 cellular defense response
- GO:0007202 activation of phospholipase C activity
- GO:0007204 elevation of cytosolic calcium ion concentration
- GO:0007606 sensory perception of chemical stimulus
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