CD88 antibody | P12/1

Mouse anti Human CD88

Product Type
Monoclonal Antibody
Clone
P12/1
Isotype
IgG2a
Specificity
CD88

Product Code Applications Pack Size List Price Your Price Qty
MCA2059
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F P WB 0.2 mg loader
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loader
MCA2059T
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SDS Safety Datasheet SDS
C F P WB 25 µg loader
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loader
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Mouse anti Human CD88 antibody, clone P12/1 recognizes the C5a receptor (C5aR) also known as CD88 or C5a anaphylatoxin chemotactic receptor 1. CD88 is predominantly expressed on cells of the myeloid lineage.

When C5aR is preincubated with C5a, Mouse anti Human CD88 antibody, clone P12/1 does not bind to the receptor, as the binding site of P12/1 is located in the C5a binding region (Werfel et al. 1996 and Weinman et al. 2003)

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rhesus Monkey
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
C5aR - peptide: Met1 - Asn31
Approx. Protein Concentrations
IgG concentration 1 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse X63 - Ag8 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/50 1/100
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 5 x 105 cells in 100ul.

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Description Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (Fc):FITC
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Description Goat anti Mouse IgG (Fc):HRP
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Description Rabbit F(ab')2 anti Mouse IgG:RPE
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Description Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
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Description Goat anti Mouse IgG:FITC (Rat Adsorbed)
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Description Goat anti Mouse IgG:RPE (Rat Adsorbed)
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
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Description Goat anti Mouse IgG:HRP (Rat Adsorbed)
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
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Description Goat anti Mouse IgG/A/M:Alk. Phos.
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
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Description Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
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Description Rabbit F(ab')2 anti Mouse IgG:Dylight®800
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader
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Description Rabbit F(ab')2 anti Mouse IgG:FITC

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG2a Negative Control MCA929 F 100 Tests loader
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Description Mouse IgG2a Negative Control

References for CD88 antibody

  1. Werfel, T. et al. (1996) CD88 antibodies specifically bind to C5aR on dermal CD117+ and CD14+ cells and react with a desmosomal antigen in human skin.
    J Immunol. 157: 1729-35.
  2. Opperman, M. et al. (1995) Antibodies from the myeloid panel that react with the C5a receptor and anatagonize C5a biological activity.
    In: Schlossman, S.F. (ed.) Leucocyte Typing V. OUP: 955-6.
  3. Pollok-Kopp B et al. (2007) Dynamics of protein kinase C-mediated phosphorylation of the complement C5a receptor on serine 334.
    J Biol Chem. 282 (7): 4345-53.
  4. Werfel, T. et al. (1995) Binding of anti-C5a receptor (C5aR) antibodies to cells of clinically normal human skin.
    In: Schlossman, S.F. (ed.) Leucocyte Typing V. OUP: 957-9.
  5. Oppermann, M. (1995) Cluster report: (C5a receptor).
    In: Schlossman, S.F. (ed.) Leucocyte Typing V. OUP: 953-4.
  6. Hüttenrauch, F. et al. (2005) G protein-coupled receptor kinases promote phosphorylation and beta-arrestin-mediated internalization of CCR5 homo- and hetero-oligomers.
    J Biol Chem. 280: 37503-15.
  7. Huber-Lang, M. et al. (2005) Changes in the novel orphan, C5a receptor (C5L2), during experimental sepsis and sepsis in humans.
    J Immunol. 174 (2): 1104-10.
  8. Morris, A.C. et al. (2011) C5a-mediated neutrophil dysfunction is RhoA-dependent and predicts infection in critically ill patients.
    Blood. 117: 5178-88.
  9. View The Latest Product References
  10. Visser T et al. (2012) Homology in systemic neutrophil response induced by human experimental endotoxemia and by trauma.
    Shock. 37 (2): 145-51.
  11. Nitta, H. et al. (2013) Enhancement of human cancer cell motility and invasiveness by anaphylatoxin C5a via aberrantly expressed C5a receptor (CD88).
    Clin Cancer Res. 19 (8): 2004-13.
  12. Patzelt, J. et al. (2015) Expression of anaphylatoxin receptors on platelets in patients with coronary heart disease.
    Atherosclerosis. 238 (2): 289-95.
  13. Unnewehr H et al. (2013) Changes and regulation of the C5a receptor on neutrophils during septic shock in humans.
    J Immunol. 190 (8): 4215-25.
  14. Visser T et al. (2011) Isolated blunt chest injury leads to transient activation of circulating neutrophils.
    Eur J Trauma Emerg Surg. 37 (2): 177-84.
  15. Presicce, P. et al. (2015) Neutrophil recruitment and activation in decidua with intra-amniotic IL-1beta in the preterm rhesus macaque.
    Biol Reprod. 92 (2): 56.
  16. Zannetti, C. et al. (2016) Characterization of the Inflammasome in Human Kupffer Cells in Response to Synthetic Agonists and Pathogens.
    J Immunol. 197 (1): 356-67.
  17. Werfel, T. et al. (1996) The human mast cell line HMC-1 expresses C5a receptors and responds to C5a but not to C5a(desArg).
    Scand J Immunol. 44 (1): 30-6.
  18. Weinmann, O. et al. (2003) Up-regulation of C5a receptor expression and function on human monocyte derived dendritic cells by prostaglandin E2.
    Immunology. 110 (4): 458-65.
  19. Hodille, E. et al. (2020) Staphylococcal Panton–Valentine Leucocidin and Gamma Haemolysin Target and Lyse Mature Bone Marrow Leucocytes.
    Toxins. 12 (11): 725.
  20. Henes, J.K. et al. (2021) C5 Variant rs10985126 is Associated with Mortality in Patients with Symptomatic Coronary Artery Disease.
    Pharmgenomics Pers Med. 14: 893-903.
  21. Imamura, R. et al. (2021) Prostate cancer C5a receptor expression and augmentation of cancer cell proliferation, invasion, and PD-L1 expression by C5a.
    Prostate. 81 (3): 147-156.

Further Reading

  1. Oppermann, M. et al. (1993) Probing the human receptor for C5a anaphylatoxin with site-directed antibodies. Identification of a potential ligand binding site on the NH2-terminal domain.
    J Immunol. 151 (7): 3785-94.
  2. Oppermann, M. & Götze, O. (1994) Plasma clearance of the human C5a anaphylatoxin by binding to leucocyte C5a receptors.
    Immunology. 82 (4): 516-21.

Western Blotting

Synonyms
C5aR
RRID
AB_566906
UniProt
P21730
Entrez Gene
C5AR1
GO Terms
GO:0000187 activation of MAPK activity
GO:0004944 C5a anaphylatoxin receptor activity
GO:0005887 integral to plasma membrane
GO:0006955 immune response
GO:0006968 cellular defense response
GO:0007202 activation of phospholipase C activity
GO:0007204 elevation of cytosolic calcium ion concentration
GO:0007606 sensory perception of chemical stimulus
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