CD88 antibody | P12/1
Fitc conjugated Mouse anti Human CD88 antibody, clone P12/1 (MCA2059F) used for the evaluation of C5aR expression on human bone marrow leukocytes by flowcytometry.
Image caption:
Analysis of C5aR expression on bone marrow leucocytes subpopulation. Expression was assessed using monoclonal antibodies coupled to fluorescein isothiocyanate (FITC) compared to isotypic control. The ratio median fluorescence intensity (RMFI) is the ratio between the median of the intensity of the fluorescence of specific monoclonal antibodies (purple for polymorphonuclear neutrophil cells (PMNs), green for Ly and blue for CD11b+ Mo) and the median of the intensity of the fluorescence of the isotypic control (grey). PMN, polymorphonuclear neutrophil cells; Ly, lymphocytes; CD11b+ mo, CD11b+ monocytes.
From: Hodille, E.; Plesa, A.; Bourrelly, E.; Belmont, L.; Badiou, C.; Lina, G.; Dumitrescu, O.
Staphylococcal Panton–Valentine Leucocidin and Gamma Haemolysin Target and Lyse Mature Bone Marrow Leucocytes.
Toxins 2020, 12, 725.
doi: 10.3390/toxins12110725This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human CD88
- Product Type
- Monoclonal Antibody
- Clone
- P12/1
- Isotype
- IgG2a
- Specificity
- CD88
Antibody Quality and Performance Guarantee
| Mouse anti Human CD88 antibody, clone P12/1 recognizes the C5a receptor (C5aR) also known as CD88 or C5a anaphylatoxin chemotactic receptor 1. CD88 is predominantly expressed on cells of the myeloid lineage. When C5aR is preincubated with C5a, Mouse anti Human CD88 antibody, clone P12/1 does not bind to the receptor, as the binding site of P12/1 is located in the C5a binding region (Werfel et al. 1996 and Weinman et al. 2003) |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rhesus Monkey - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- C5aR - peptide: Met1 - Asn31
- Approx. Protein Concentrations
- IgG concentration 1 mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the mouse X63 - Ag8 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/50 | 1/100 |
| Immunohistology - Frozen | |
||
| Immunohistology - Paraffin | |
||
| Immunoprecipitation | |||
| Western Blotting | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 5 x 105 cells in 100μl
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG2a Negative Control | MCA929 | F | 100 Tests |
|
Log in | ||
| List Price | Your Price | ||||||
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Log in | ||||||
| Description | Mouse IgG2a Negative Control | ||||||
Source Reference
-
Werfel, T. et al. (1996) CD88 antibodies specifically bind to C5aR on dermal CD117+ and CD14+ cells and react with a desmosomal antigen in human skin.
J Immunol. 157: 1729-35.
References for CD88 antibody
-
Werfel, T. et al. (1995) Binding of anti-C5a receptor (C5aR) antibodies to cells of clinically normal human skin.
In: Schlossman, S.F. (ed.) Leucocyte Typing V. OUP: 957-9. -
Oppermann, M. (1995) Cluster report: (C5a receptor).
In: Schlossman, S.F. (ed.) Leucocyte Typing V. OUP: 953-4. -
Opperman, M. et al. (1995) Antibodies from the myeloid panel that react with the C5a receptor and anatagonize C5a biological activity.
In: Schlossman, S.F. (ed.) Leucocyte Typing V. OUP: 955-6. -
Werfel, T. et al. (1996) The human mast cell line HMC-1 expresses C5a receptors and responds to C5a but not to C5a(desArg).
Scand J Immunol. 44 (1): 30-6. -
Weinmann, O. et al. (2003) Up-regulation of C5a receptor expression and function on human monocyte derived dendritic cells by prostaglandin E2.
Immunology. 110 (4): 458-65. -
Hüttenrauch, F. et al. (2005) G protein-coupled receptor kinases promote phosphorylation and beta-arrestin-mediated internalization of CCR5 homo- and hetero-oligomers.
J Biol Chem. 280: 37503-15. -
Huber-Lang, M. et al. (2005) Changes in the novel orphan, C5a receptor (C5L2), during experimental sepsis and sepsis in humans.
J Immunol. 174 (2): 1104-10. -
Pollok-Kopp B et al. (2007) Dynamics of protein kinase C-mediated phosphorylation of the complement C5a receptor on serine 334.
J Biol Chem. 282 (7): 4345-53.
View The Latest Product References
-
Visser T et al. (2011) Isolated blunt chest injury leads to transient activation of circulating neutrophils.
Eur J Trauma Emerg Surg. 37 (2): 177-84. -
Morris, A.C. et al. (2011) C5a-mediated neutrophil dysfunction is RhoA-dependent and predicts infection in critically ill patients.
Blood. 117: 5178-88. -
Visser T et al. (2012) Homology in systemic neutrophil response induced by human experimental endotoxemia and by trauma.
Shock. 37 (2): 145-51. -
Nitta, H. et al. (2013) Enhancement of human cancer cell motility and invasiveness by anaphylatoxin C5a via aberrantly expressed C5a receptor (CD88).
Clin Cancer Res. 19 (8): 2004-13. -
Unnewehr H et al. (2013) Changes and regulation of the C5a receptor on neutrophils during septic shock in humans.
J Immunol. 190 (8): 4215-25. -
Presicce, P. et al. (2015) Neutrophil recruitment and activation in decidua with intra-amniotic IL-1beta in the preterm rhesus macaque.
Biol Reprod. 92 (2): 56. -
Patzelt, J. et al. (2015) Expression of anaphylatoxin receptors on platelets in patients with coronary heart disease.
Atherosclerosis. 238 (2): 289-95. -
Zannetti, C. et al. (2016) Characterization of the Inflammasome in Human Kupffer Cells in Response to Synthetic Agonists and Pathogens.
J Immunol. 197 (1): 356-67. -
Hodille, E. et al. (2020) Staphylococcal Panton–Valentine Leucocidin and Gamma Haemolysin Target and Lyse Mature Bone Marrow Leucocytes.
Toxins. 12 (11): 725. -
Henes, J.K. et al. (2021) C5 Variant rs10985126 is Associated with Mortality in Patients with Symptomatic Coronary Artery Disease.
Pharmgenomics Pers Med. 14: 893-903. -
Imamura, R. et al. (2021) Prostate cancer C5a receptor expression and augmentation of cancer cell proliferation, invasion, and PD-L1 expression by C5a.
Prostate. 81 (3): 147-156. -
Newman, J. et al. (2024) Chorioamnionitis accelerates granule cell and oligodendrocyte maturation in the cerebellum of preterm nonhuman primates.
J Neuroinflammation. 21 (1): 16.
Further Reading
-
Oppermann, M. et al. (1993) Probing the human receptor for C5a anaphylatoxin with site-directed antibodies. Identification of a potential ligand binding site on the NH2-terminal domain.
J Immunol. 151 (7): 3785-94. -
Oppermann, M. & Götze, O. (1994) Plasma clearance of the human C5a anaphylatoxin by binding to leucocyte C5a receptors.
Immunology. 82 (4): 516-21.
- Synonyms
- C5aR
- RRID
- AB_566906
- UniProt
- P21730
- Entrez Gene
- C5AR1
- GO Terms
- GO:0000187 activation of MAPK activity
- GO:0004944 C5a anaphylatoxin receptor activity
- GO:0005887 integral to plasma membrane
- GO:0006955 immune response
- GO:0006968 cellular defense response
- GO:0007202 activation of phospholipase C activity
- GO:0007204 elevation of cytosolic calcium ion concentration
- GO:0007606 sensory perception of chemical stimulus
MCA2059
154487
163541
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