CD88 antibody | P12/1
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|Mouse anti Human CD88 antibody, clone P12/1 recognizes the C5a receptor (C5aR) also known as CD88 or C5a anaphylatoxin chemotactic receptor 1. CD88 is predominantly expressed on cells of the myeloid lineage.
When C5aR is preincubated with C5a, Mouse anti Human CD88 antibody, clone P12/1 does not bind to the receptor, as the binding site of P12/1 is located in the C5a binding region (Werfel et al. 1996 and Weinman et al. 2003)
- Target Species
- Species Cross-Reactivity
Target Species Cross Reactivity Rhesus Monkey
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide
- Carrier Free
- C5aR - peptide: Met1 - Asn31
- Approx. Protein Concentrations
- IgG concentration 1 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse X63 - Ag8 myeloma cell line.
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Frozen|
|Immunohistology - Paraffin|
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 5 x 105 cells in 100ul.
References for CD88 antibody
Werfel, T. et al. (1996) CD88 antibodies specifically bind to C5aR on dermal CD117+ and CD14+ cells and react with a desmosomal antigen in human skin.
J Immunol. 157: 1729-35.
Opperman, M. et al. (1995) Antibodies from the myeloid panel that react with the C5a receptor and anatagonize C5a biological activity.
In: Schlossman, S.F. (ed.) Leucocyte Typing V. OUP: 955-6.
Pollok-Kopp B et al. (2007) Dynamics of protein kinase C-mediated phosphorylation of the complement C5a receptor on serine 334.
J Biol Chem. 282 (7): 4345-53.
Werfel, T. et al. (1995) Binding of anti-C5a receptor (C5aR) antibodies to cells of clinically normal human skin.
In: Schlossman, S.F. (ed.) Leucocyte Typing V. OUP: 957-9.
Oppermann, M. (1995) Cluster report: (C5a receptor).
In: Schlossman, S.F. (ed.) Leucocyte Typing V. OUP: 953-4.
Hüttenrauch, F. et al. (2005) G protein-coupled receptor kinases promote phosphorylation and beta-arrestin-mediated internalization of CCR5 homo- and hetero-oligomers.
J Biol Chem. 280: 37503-15.
Huber-Lang, M. et al. (2005) Changes in the novel orphan, C5a receptor (C5L2), during experimental sepsis and sepsis in humans.
J Immunol. 174 (2): 1104-10.
Morris, A.C. et al. (2011) C5a-mediated neutrophil dysfunction is RhoA-dependent and predicts infection in critically ill patients.
Blood. 117: 5178-88.
View The Latest Product References
Visser T et al. (2012) Homology in systemic neutrophil response induced by human experimental endotoxemia and by trauma.
Shock. 37 (2): 145-51.
Nitta, H. et al. (2013) Enhancement of human cancer cell motility and invasiveness by anaphylatoxin C5a via aberrantly expressed C5a receptor (CD88).
Clin Cancer Res. 19 (8): 2004-13.
Patzelt, J. et al. (2015) Expression of anaphylatoxin receptors on platelets in patients with coronary heart disease.
Atherosclerosis. 238 (2): 289-95.
Unnewehr H et al. (2013) Changes and regulation of the C5a receptor on neutrophils during septic shock in humans.
J Immunol. 190 (8): 4215-25.
Visser T et al. (2011) Isolated blunt chest injury leads to transient activation of circulating neutrophils.
Eur J Trauma Emerg Surg. 37 (2): 177-84.
Presicce, P. et al. (2015) Neutrophil recruitment and activation in decidua with intra-amniotic IL-1beta in the preterm rhesus macaque.
Biol Reprod. 92 (2): 56.
Zannetti, C. et al. (2016) Characterization of the Inflammasome in Human Kupffer Cells in Response to Synthetic Agonists and Pathogens.
J Immunol. 197 (1): 356-67.
Werfel, T. et al. (1996) The human mast cell line HMC-1 expresses C5a receptors and responds to C5a but not to C5a(desArg).
Scand J Immunol. 44 (1): 30-6.
Weinmann, O. et al. (2003) Up-regulation of C5a receptor expression and function on human monocyte derived dendritic cells by prostaglandin E2.
Immunology. 110 (4): 458-65.
Hodille, E. et al. (2020) Staphylococcal Panton–Valentine Leucocidin and Gamma Haemolysin Target and Lyse Mature Bone Marrow Leucocytes.
Toxins. 12 (11): 725.
Henes, J.K. et al. (2021) C5 Variant rs10985126 is Associated with Mortality in Patients with Symptomatic Coronary Artery Disease.
Pharmgenomics Pers Med. 14: 893-903.
Imamura, R. et al. (2021) Prostate cancer C5a receptor expression and augmentation of cancer cell proliferation, invasion, and PD-L1 expression by C5a.
Prostate. 81 (3): 147-156.
Oppermann, M. et al. (1993) Probing the human receptor for C5a anaphylatoxin with site-directed antibodies. Identification of a potential ligand binding site on the NH2-terminal domain.
J Immunol. 151 (7): 3785-94.
Oppermann, M. & Götze, O. (1994) Plasma clearance of the human C5a anaphylatoxin by binding to leucocyte C5a receptors.
Immunology. 82 (4): 516-21.
- Entrez Gene
- GO Terms
- GO:0000187 activation of MAPK activity
- GO:0004944 C5a anaphylatoxin receptor activity
- GO:0005887 integral to plasma membrane
- GO:0006955 immune response
- GO:0006968 cellular defense response
- GO:0007202 activation of phospholipase C activity
- GO:0007204 elevation of cytosolic calcium ion concentration
- GO:0007606 sensory perception of chemical stimulus
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