CD87 antibody | VIM-5

Mouse anti Human CD87

Product Type
Monoclonal Antibody
Clone
VIM-5
Isotype
IgG1
Specificity
CD87

Product Code Applications Pack Size List Price Your Price Qty
MCA2516GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F IP 0.1 mg loader
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loader
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Mouse anti Human CD87 antibody, clone VIM-5 recognizes human CD87, also known as urokinase plasminogen activator receptor (uPAR), a 50-65 kDa single chain glycosylphostidylinsitol (GPI) anchored glycoprotein, expressed by granulocytes, tissue macrophages, monocytes, endothelial cells, fibroblasts and mesenchymal and epithelial tumour cells.

CD87 specifically binds and activates urokinase plasminogen activator (uPA), a serine protease which converts the inactive receptor-bound proenzyme plasminogen into plasmin, triggering the proteolysis cascade of fibrinolysis. The interaction between uPAR and uPA is also known to play a role in cancer cell invasion and metastasis.

Clone VIM-5 is reported to block urokinase binding.

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
THP-1 cells (Human acute monocytic leukaemia cells).
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Fusion Partners
Spleen cells from immunised Balb/c mice were fused with cells of the X63.Ag8.653 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/50 1/200
Immunohistology - Frozen 1/100
Immunoprecipitation
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Histology Positive Control Tissue
Human tonsil.

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Description Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)
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Description Goat anti Mouse IgG (Fc):FITC
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Description Goat anti Mouse IgG (Fc):HRP
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Description Rabbit F(ab')2 anti Mouse IgG:RPE
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Description Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
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Description Goat anti Mouse IgG:FITC (Rat Adsorbed)
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Description Goat anti Mouse IgG:RPE (Rat Adsorbed)
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Description Goat anti Mouse IgG:HRP (Rat Adsorbed)
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
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Description Goat anti Mouse IgG/A/M:Alk. Phos.
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
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Description Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
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Description Rabbit F(ab')2 anti Mouse IgG:Dylight®800
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader
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Description Rabbit F(ab')2 anti Mouse IgG:FITC

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control MCA928 F 100 Tests loader
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Description Mouse IgG1 Negative Control

References for CD87 antibody

  1. Sillaber, C. et al. (1997) Molecular and functional characterization of the urokinase receptor on human mast cells.
    J Biol Chem. 272 (12): 7824-32.
  2. Gadd, S.J. et al. (1990) M5, a phosphoinositol-linked human myelomonocytic activation-associated antigen.
    Clin Exp Immunol. 80 (2): 252-6.

Further Reading

  1. Mondino, A. & Blasi, F. (2004) uPA and uPAR in fibrinolysis, immunity and pathology.
    Trends Immunol. 25 (8): 450-5.

Synonyms
UPAR
UPA-R
RRID
AB_915235
UniProt
Q03405
Entrez Gene
PLAUR
View more products with CD87 specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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