CD85k antibody | rA43B43-02/1F6-3
Mouse anti Human CD85k
- Product Type
- Monoclonal Antibody
- Clone
- rA43B43-02/1F6-3
- Isotype
- IgG1
- Specificity
- CD85k
Antibody Quality and Performance Guarantee
TZA0164GA
Recombinant Antibodies
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| Mouse anti Human CD85k antibody, clone rA43B43-02/1F6-3, is a recombinant monoclonal antibody that recognizes CD85k, also known as Immunoglobulin-like transcript 3 (ILT-3) and leukocyte immunoglobulin-like receptor subfamily B member 4 (LILRB4). CD85k is a type I transmembrane glycoprotein, part of the LILR family of innate immunomodulatory receptors. It contains two extracellular immunoglobulin-like domains and three cytoplasmic immunoreceptor tyrosine-based inhibitor motifs (ITIM) that exert an inhibitory effect on immune responses. CD85k is expressed by dendritic cells, monocytes and macrophages and is stimulated by a range of ligands, including APOE, CD166 and fibronectin. Upon ligand binding, the Src-family protein Lyn is autophosphorylated, phosphorylating the ITIM tyrosine residues and leading to negative regulation of Syk and PI3K signaling. CD85k has roles in the induction of tolerogenic dendritic cells and regulatory T cells, inhibition of cytokine production and inhibition of T cell activity. CD85k is expressed on a number of cancers, including multiple myeloma, gastric cancer, AML and ovarian cancer. It is a potential therapeutic target in cancer immunotherapy. |
- Target Species
- Human
- Product Form
- Purified recombinant IgG containing a SpyTag at the C-terminus of the antibody heavy chain – liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Source
- CHO cells
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Recombinant CD85k Gln22-Glu259 expressed by mammalian cells
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 5μl of the suggested working dilution to label 0.5x106 cells in 100μl. Best practices suggest a 5 min centrifugation at 6,000g prior to sample application.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control | ||||||
- Synonyms
- ILT-3
- UniProt
- Q8NHJ6
- Entrez Gene
- LILRB4
- GO Terms
- GO:0005886 plasma membrane
- GO:0003823 antigen binding
- GO:0016021 integral to membrane
- GO:0004872 receptor activity
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