CD81 antibody | 1D6
Mouse anti Human CD81 antibody, clone 1D6 (MCA1847) used to evaluate CD81 expression in £T£-L1 cell lysates by western blotting.
Image caption:
Characterization of EVs released by 3T3‐L1 adipocytes. A) Cryo‐electron microscopy from Control (untreated), hypoxic or cells exposed to macrophage media (MCM). Scale bar: 100 nm. B) NTA of EVs from normoxic/ hypoxic cells and control/MCM‐exposed 3T3L1 adipocytes. C) Immunoblot of cellular lysates (L) or EVs from control, hypoxic, or MCM exposed cells. Equal amounts of lysate or EVs (1 μg) were separated by SDS‐PAGE and immunoblotted with specific antibodies as indicated.
From: Mleczko J, Ortega FJ, Falcon-Perez JM, Wabitsch M, Fernandez-Real JM, Mora S.
Extracellular Vesicles from Hypoxic Adipocytes and Obese Subjects Reduce Insulin-Stimulated Glucose Uptake.
Mol Nutr Food Res. 2018 Mar;62(5). Epub 2018 Feb 20.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Human CD81 antibody, clone 1D6 (MCA1847) used to evaluate CD81 expression in human extracellular vesicles by western blotting.
Image caption:
A) EVs from plasma (EV blood), EVs from human adipocytes (EV adipo), and adipocyte cellular lysates were separated by SDS‐PAGE and immunoblotted with antibodies for EV markers as indicated. B) Size distribution of plasma EVs by NTA. C) Insulin‐stimulated 2‐deoxyglucose uptake in SGBS cells treated with 2 × 105 EV mL–1 of EVs obtained from lean (n = 8) or obese subjects (n = 9). Data shows mean ± SD EV, each EV was tested in triplicate in two independent experiments, p = 0.0153 (t‐test).
From: Mleczko J, Ortega FJ, Falcon-Perez JM, Wabitsch M, Fernandez-Real JM, Mora S.
Extracellular Vesicles from Hypoxic Adipocytes and Obese Subjects Reduce Insulin-Stimulated Glucose Uptake.
Mol Nutr Food Res. 2018 Mar;62(5). Epub 2018 Feb 20.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Filter by Application:
F WB ResetMouse anti Human CD81
- Product Type
- Monoclonal Antibody
- Clone
- 1D6
- Isotype
- IgG1
- Specificity
- CD81
Antibody Quality and Performance Guarantee
| Mouse anti Human CD81 antibody, clone 1D6 recognizes human CD81, a 26 kDa cell surface antigen also known as TAPA-1, and a member of the tetraspanin family. CD81 is widely expressed on human leucocytes and appears to be involved in a variety of cellular leucocytes including activation, proliferation and differentiation. Mouse anti Human CD81 antibody, clone 1D6 is a potent CD81 reagent, induces homotypic adhesion and has powerful anti-proliferative effects. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Chimpanzee Sheep Goat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- OCI-LY8 cells aggregated by 5A6 (another CD81 antibody)
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse PX3-Ag.8.653 myeloma cell line
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry 1 |  |
1/5 | 1/10 |
| Immunofluorescence | |
||
| Immunohistology - Paraffin | |
||
| Immunoprecipitation | |
||
| Western Blotting | |
- 1Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
|
Log in | ||
| List Price | Your Price | ||||||
|
|
Log in | ||||||
| Description | Mouse IgG1 Negative Control | ||||||
References for CD81 antibody
-
Schick, M.R. & Levy, S. (1993) The TAPA-1 molecule is associated on the surface of B cells with HLA-DR molecules.
J Immunol. 151 (8): 4090-7. -
Levy, S. et al. (1998) CD81 (TAPA-1): a molecule involved in signal transduction and cell adhesion in the immune system.
Annu Rev Immunol. 16: 89-109. -
Griebel, P.J. et al. (2007) Cross-reactivity of mAbs to human CD antigens with sheep leukocytes.
Vet Immunol Immunopathol. 119: 115-22. -
Welton, J.L. et al (2010) Proteomics analysis of bladder cancer exosomes.
Mol Cell Proteomics. 9: 1324-38. -
Davis, W.C. et al. (2007) Use of flow cytometry to identify monoclonal antibodies that recognize conserved epitopes on orthologous leukocyte differentiation antigens in goats, llamas, and rabbits.
Vet Immunol Immunopathol. 119: 123-30. -
Flint, M. et al. (1999) Characterization of hepatitis C virus E2 glycoprotein interaction with a putative cellular receptor, CD81.
J Virol. 73:6235-44. -
Parthasarathy, V. et al. (2009) Distinct roles for tetraspanins CD9, CD63 and CD81 in the formation of multinucleated giant cells.
Immunology. 127: 237-48. -
Rohlena, J. et al. (2009) Endothelial CD81 is a marker of early human atherosclerotic plaques and facilitates monocyte adhesion.
Cardiovasc Res. 81: 187-96.
View The Latest Product References
-
Ventress, J.K. et al. (2016) Peptides from Tetraspanin CD9 Are Potent Inhibitors of Staphylococcus Aureus Adherence to Keratinocytes.
PLoS One. 11 (7): e0160387. -
Mleczko, J. et al. (2018) Extracellular Vesicles from Hypoxic Adipocytes and Obese Subjects Reduce Insulin-Stimulated Glucose Uptake.
Mol Nutr Food Res. 62 (5)Feb 20 [Epub ahead of print].
- Synonyms
- TAPA-1
- RRID
- AB_323286
- UniProt
- P60033
- Entrez Gene
- CD81
- GO Terms
- GO:0000187 activation of MAPK activity
- GO:0005515 protein binding
- GO:0008104 protein localization
- GO:0005887 integral to plasma membrane
- GO:0006661 phosphatidylinositol biosynthetic process
- GO:0008283 cell proliferation
- GO:0008284 positive regulation of cell proliferation
- GO:0043128 positive regulation of 1-phosphatidylinositol 4-kinase activity
- GO:0046813 virion attachment, binding of host cell surface receptor
- View More GO Terms
- GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation
- GO:0050776 regulation of immune response
MCA1847
151281
MCA1847T
150389
153047
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
View more products with CD81 specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
View all Anti-Human ProductsAlways be the first to know.
When we launch new products and resources to help you achieve more in the lab.
Yes, sign me up