CD80 antibody | rA11B11-01/2A7
Mouse anti Human CD80
- Product Type
- Monoclonal Antibody
- Clone
- rA11B11-01/2A7
- Isotype
- IgG1
- Specificity
- CD80
Antibody Quality and Performance Guarantee
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| Mouse anti Human CD80 antibody, clone rA11B11-01/2A7, is a recombinant monoclonal antibody that recognizes CD80, also known as B-lymphocyte activation antigen B7 or B7-1. CD80 is a member of the immunoglobulin superfamily, consisting of an extracellular domain bearing two Ig-like domains, a transmembrane sequence, and a short cytoplasmic domain. CD80 is expressed by macrophages, dendritic cells, T cells and activated B-cells. CD80, along with CD86, act as co-stimulatory molecules and are both ligands for CD28 and CTLA-4. Binding to CD28 induces T-cell proliferation, whereas binding to CTLA-4 inhibits T-cell activation. Although CD80 binds to the same receptors as CD86, it displays different characteristics in its avidity and binding kinetics. CD80 is also expressed on the surface of tumor cells. It binds PD-L1, promoting T cell responses by interfering in the interaction between tumor cells and T cells. CD80 has been proposed as an immunotherapy target. |
- Target Species
- Human
- Product Form
- Purified recombinant IgG containing a SpyTag at the C-terminus of the antibody heavy chain – liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Source
- CHO cells
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Recombinant CD80 (Val35-Asn242) expressed by mammalian cells
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 5μl of the suggested working dilution to label 0.5x106 cells in 100μl. Best practices suggest a 5 min centrifugation at 6,000g prior to sample application.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control | ||||||
- Synonyms
- B7-1
- UniProt
- P33681
- Entrez Gene
- CD80
- GO Terms
- GO:0005515 protein binding
- GO:0016021 integral to membrane
- GO:0035556 intracellular signal transduction
- GO:0007267 cell-cell signaling
- GO:0009967 positive regulation of signal transduction
- GO:0015026 coreceptor activity
- GO:0016563 transcription activator activity
- GO:0031295 T cell costimulation
- GO:0042110 T cell activation
- View More GO Terms
- GO:0044419 interspecies interaction between organisms
- GO:0045086 positive regulation of interleukin-2 biosynthetic process
- GO:0045425 positive regulation of granulocyte macrophage colony-stimulating factor biosynthetic process
- GO:0045627 positive regulation of T-helper 1 cell differentiation
- GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation
TZA077GA
64702102
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