CD80 antibody | MEM-233

Mouse anti Human CD80

Product Type
Monoclonal Antibody
Clone
MEM-233
Isotype
IgG1
Specificity
CD80

Product Code Applications Pack Size List Price Your Price Qty
MCA2071GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F IP WB 0.1 mg loader
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loader
MCA2071
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SDS Safety Datasheet SDS
F IP WB 0.2 mg loader
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loader
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Mouse anti Human CD80 antibody, clone MEM-233 recognizes human CD80, also known as B7-1, a ~60 kDa type 1 trans-membrane protein expressed of macrophages, dendritic cells (Munro et al. 1994) and activated B-cells (Ranheim et al. 1993). CD80 is a member of the immunoglobulin superfamily having an extracellular domain bearing both a single Ig-v-like domain, a single Ig-c-like domain, a transmembrane sequence and a short cytoplasmic domain. Although the predicted molecular weight for human CD80 is ~33 kDa, the presence of multiple (8) potential N-glycosylation sites (Chen et al. 1998) results in a migration corresponding to ~60 kDa.

Human CD80 along with CD86 act as co-stimulatory molecules and are both ligands for CD28 and CTLA-4 (Azuma et al. 1993) involved in T cell activation and proliferation (Vasu et al. 2003). Although CD80 binds to the same receptors as CD86 it displays quite different characteristics in its avidity and binding kinetics (van der Merwe et al. 1997).

Mutagenesis indicates residues in both the Ig-v-like and Ig-c-like domains of CD80 are crucial for the interaction with it’s receptors CTLA-4 and CD28 (Peach et al. 1995).

Mouse anti human CD80 antibody, clone MEM-233 binds to residues within the Ig-v-like domain of human CD80 as shown by domain switching assays (Vasu et al. 2003).

Mouse anti Human CD80, clone MEM-233 in combination with Mouse anti Human CD86, clone Bu63 (MCA1118) suggest that clone MEM-233 is able to block binding of human CD80 with it's cognate ligands CD28 and CTLA-4 (Morbach et al. 2011).

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Immunoprecipitation
Western Blotting Non-reducing conditions
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells or 1007mu;l whole blood

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References for CD80 antibody

  1. Zhan, H. et al. (2003) The immunomodulatory role of human conjunctival epithelial cells.
    Invest Ophthalmol Vis Sci. 44 (9): 3906-10.
  2. Angel, C.E. et al. (2006) Cutting edge: CD1a+ antigen-presenting cells in human dermis respond rapidly to CCR7 ligands.
    J Immunol. 176 (10): 5730-4.
  3. Daubenberger, C.A. et al. (2007) Flow cytometric analysis on cross-reactivity of human-specific CD monoclonal antibodies with splenocytes of
    Aotus nancymaae, a non-human primate model for biomedical research.
    Vet Immunol Immunopathol. 119 (1-2): 14-20.
  4. Hovden, A.O. et al. (2011) Maturation of monocyte derived dendritic cells with OK432 boosts IL-12p70 secretion and conveys strong T-cell responses.
    BMC Immunol. 12: 2.
  5. John, J. et al. (2010) Differential effects of Paclitaxel on dendritic cell function.
    BMC Immunol. 11: 14.
  6. Silk, K.M. et al. (2012) Cross-presentation of tumour antigens by human induced pluripotent stem cell-derived CD141+XCR1+ dendritic cells
    Gene Ther. 19: 1035-40.
  7. Piconi, S. et al. (2010) Immunological effects of sublingual immunotherapy: clinical efficacy is associated with modulation of programmed cell death ligand 1, IL-10, and IgG4.
    J Immunol. 185: 7723-30.
  8. Tan, P.H. et al. (2004) Phenotypic and functional differences between human saphenous vein (HSVEC) and umbilical vein (HUVEC) endothelial cells.
    Atherosclerosis. 173: 171-83.
  9. View The Latest Product References
  10. Tan, P.H. et al. (2005) Modulation of human dendritic-cell function following transduction with viral vectors: implications for gene therapy.
    Blood. 105: 3824-32.
  11. Trojan, J. et al. (2010) Antisense anti IGF-I cellular therapy of malignant tumours: immune response in cancer patients.
    Biomed Pharmacother. 64: 576-8.
  12. Huxley, P. et al. (2004) High-affinity small molecule inhibitors of T cell costimulation: compounds for immunotherapy.
    Chem Biol. 11: 1651-8.
  13. Silk, K.M. et al. (2012) Rapamycin conditioning of dendritic cells differentiated from human ES cells promotes a tolerogenic phenotype.
    J Biomed Biotechnol. 2012: 172420.
  14. Scott-Taylor, T.H. et al. (2017) Enhanced formation of giant cells in common variable immunodeficiency: Relation to granulomatous disease.
    Clin Immunol. 175: 1-9.
  15. Demmers, M.W. et al. (2013) Differential effects of activated human renal epithelial cells on T-cell migration.
    PLoS One. 8 (5): e64916.

Flow Cytometry

Synonyms
B7-1
RRID
AB_323471
UniProt
P33681
Entrez Gene
CD80
GO Terms
GO:0005515 protein binding
GO:0016021 integral to membrane
GO:0035556 intracellular signal transduction
GO:0007267 cell-cell signaling
GO:0009967 positive regulation of signal transduction
GO:0015026 coreceptor activity
GO:0016563 transcription activator activity
GO:0031295 T cell costimulation
GO:0042110 T cell activation
GO:0044419 interspecies interaction between organisms
GO:0045086 positive regulation of interleukin-2 biosynthetic process
GO:0045425 positive regulation of granulocyte macrophage colony-stimulating factor biosynthetic process
GO:0045627 positive regulation of T-helper 1 cell differentiation
GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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