CD8 antibody | RPA-T8
Mouse anti Human CD8
- Product Type
- Monoclonal Antibody
- Clone
- RPA-T8
- Isotype
- IgG1
- Specificity
- CD8
Antibody Quality and Performance Guarantee
| Mouse anti Human CD8 monoclonal antibody, clone RPA-T8 recognizes the alpha-chain of the human CD8 antigen, a ~32 kDa single pass type 1 membrane glycoprotein containing a single Ig-like V-type domain. CD8α forms either homodimers or disulphide bond linked heterodimers with the CD8β chain. CD8 is expressed by thymocytes, the cytotoxic-suppressor subset of T cells and weakly by NK cells. CD8 functions as a receptor for MHC Class I molecules. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Chimpanzee Baboon Cynomolgus monkey Rhesus Monkey Macaque - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Approx. Protein Concentrations
- IgG concentration 0.5mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/5 | 1/20 |
| Immunohistology - Frozen | |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1 x 106 cells or cells or 100ul human whole blood.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control | ||||||
References for CD8 antibody
-
Morbius, U. (1989) Cluster Report: CD8
inLeukocyte Typing IV, White Cell Differentiation Antigens, edited by Knapp W, Dorken B, Gilks WR, Rieber E, Schmidt RE, Stein H, Von Dem Bome AEG, Oxford University Press: pp 475-8. -
Suranyi, M.G. et al. (1991) Lymphocyte adhesion molecules in T cell-mediated lysis of human kidney cells.
Kidney Int. 39 (2): 312-9. -
Aversa, G. et al. (1994) A monoclonal antibody (A6) recognizing a unique epitope restricted to CD45RO and RB isoforms of the leukocyte common antigen family identifies functional T cell subsets.
Cell Immunol. 158 (2): 314-28. -
SanSebastian, W. et al. (2013) Adeno-associated virus type 6 is retrogradely transported in the non-human primate brain.
Gene Ther. 20 (12): 1178-83. -
Samaranch, L. et al. (2014) AAV9-mediated expression of a non-self protein in nonhuman primate central nervous system triggers widespread neuroinflammation driven by antigen-presenting cell transduction.
Mol Ther. 22 (2): 329-37.
- RRID
- AB_10925685
- UniProt
- P01732
- P10966
- Entrez Gene
- CD8A
- CD8B
- GO Terms
- GO:0005576 extracellular region
- GO:0007169 transmembrane receptor protein tyrosine kinase signaling pathway
- GO:0005887 integral to plasma membrane
- GO:0015026 coreceptor activity
- GO:0019882 antigen processing and presentation
- GO:0042101 T cell receptor complex
- GO:0042288 MHC class I protein binding
- GO:0050776 regulation of immune response
MCA4609T
147616
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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