CD79a antibody | ZL7-4
Mouse anti Human CD79a
- Product Type
- Monoclonal Antibody
- Clone
- ZL7-4
- Isotype
- IgG1
- Specificity
- CD79a
Antibody Quality and Performance Guarantee
| Mouse anti Human CD79a antibody, clone ZL7-4 recognizes the human B-cell antigen receptor complex-associated protein alpha chain, also known as MB-1 membrane glycoprotein or CD79a. clone ZL7-4 reacts with CD79a positive cells by flow cytometry and with CD79a in an ELISA specific for a fusion protein of CD79a-Fc. Mouse anti Human CD79a antibody, clone ZL7-4 has been reported to be useful in distinguishing B-CLL from mantle cell lymphoma in flow cytometric assays (Bell et al. 1999). Mouse anti Human CD79a antibody, clone ZL7-4 has been reported to be suitable for Immunohistochemistry on frozen and pre-treated paraffin sections, but does exhibit epithelial staining. Mouse anti Human CD79a antibody, clone ZL7-4 has been reported to induce phosphorylation of syk kinase (Lanham et al. 2003). |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- TRIS buffered saline
- Preservative Stabilisers
- <0.1% sodium azide (NaN3)
- Immunogen
- IgM complex isolated from Daudi cells.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
||
| Flow Cytometry | |
10ug/ml | |
| Immunohistology - Paraffin | |
||
| Immunoprecipitation | |
20ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control | ||||||
References for CD79a antibody
-
Zhang. L. et al. (1995) The development of anti-CD79 monoclonal antibodies for treatment of B-cell neoplastic disease.
Therapeutic Immunology 2:191-202 -
Bell, P.B. et al. (1999) CD79a detected by ZL7.4 separates chronic lymphocytic leukemia from mantle cell lymphoma in the leukemic phase.
Cytometry. 38 (3): 102-5. -
Cragg, M.S. et al. (2002) The alternative transcript of CD79b is overexpressed in B-CLL and inhibits signaling for apoptosis.
Blood. 100: 3068-76. -
Lanham, S. et al. (2003) Differential signaling via surface IgM is associated with VH gene mutational status and CD38 expression in chronic lymphocytic leukemia.
Blood. 101 (3): 1087-93. -
Allsup, D.J. et al. (2005) B-cell receptor translocation to lipid rafts and associated signaling differ between prognostically important subgroups of chronic lymphocytic leukemia.
Cancer Res. 65: 7328-37. -
Rahemtullah, A. et al. (2008) CD20+ T-cell lymphoma: clinicopathologic analysis of 9 cases and a review of the literature.
Am J Surg Pathol. 32 (11): 1593-607. -
Vendel,A.C. et al (2009) B and T lymphocyte attenuator regulates B cell receptor signaling by targeting Syk and BLNK
J Immunol. 182: 1509-17.
- Synonyms
- MB-1
- RRID
- AB_321767
- UniProt
- P11912
- Entrez Gene
- CD79A
- GO Terms
- GO:0016021 integral to membrane
- GO:0004888 transmembrane receptor activity
- GO:0005771 multivesicular body
- GO:0009897 external side of plasma membrane
- GO:0019815 B cell receptor complex
- GO:0030183 B cell differentiation
- GO:0042100 B cell proliferation
- GO:0045121 membrane raft
- GO:0050853 B cell receptor signaling pathway
- View More GO Terms
MCA1298
149628
154631
167611
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