CD73 antibody | rA9-03/4C2
Mouse anti Human CD73
- Product Type
- Monoclonal Antibody
- Clone
- rA9-03/4C2
- Isotype
- IgG1
- Specificity
- CD73
Antibody Quality and Performance Guarantee
TZA078GA
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| Mouse anti Human CD73 antibody, clone rA9-03/4C2, is a recombinant monoclonal antibody that recognizes CD73, also known as Ecto-5'-nucleotidase or 5’-NT. CD73 is a cell surface GPI-anchored enzyme that catalyses the final step in the extracellular metabolism of ATP to form adenosine. It is expressed on endothelial cells, subsets of T cells and B cells, follicular dendritic cells and stromal cells. The formation of extracellular adenosine by CD73 regulates numerous signaling pathways through the activation of G protein-coupled adenosine receptors. CD73 plays a critical role in cellular homeostasis, stress responses and injury across many tissues. CD73 was originally identified as a marker of lymphocyte differentiation. CD73 is expressed by many different types of cancer and promotes tumor outgrowth, metastasis and drug resistance. High CD73 expression in tumors is associated with shorter overall survival and poor prognosis of patients in multiple cancers, including ovarian cancer, pancreatic cancer, and melanoma. Up-regulation of CD73 in breast cancer patients has been highlighted as a mechanism of resistance to therapies, with CD73 expression significantly related to poor prognosis and chemoresistance and blockade of the enzyme improving antitumor responses. CD73 expression has been indicated as both a potential prognostic factor for human solid tumors, a promising target in future cancer immunotherapy and as a potential biomarker for anti-PD-1/PD-L1 treatment. |
- Target Species
- Human
- Product Form
- Purified recombinant IgG containing a SpyTag at the C-terminus of the antibody heavy chain – liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Source
- CHO cells
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Mammalian cells transfected with full length CD73
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 5μl of the suggested working dilution to label 0.5x106 cells in 100μl. Best practices suggest a 5 min centrifugation at 6,000g prior to sample application.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control | ||||||
- Synonyms
- 5'-NT
- UniProt
- P21589
- Entrez Gene
- NT5E
- GO Terms
- GO:0005886 plasma membrane
- GO:0000166 nucleotide binding
- GO:0005624 membrane fraction
- GO:0005737 cytoplasm
- GO:0006144 purine base metabolic process
- GO:0006206 pyrimidine base metabolic process
- GO:0006259 DNA metabolic process
- GO:0008253 5'-nucleotidase activity
- GO:0031225 anchored to membrane
- View More GO Terms
- GO:0046135 pyrimidine nucleoside catabolic process
TZA078GA
64714518
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