CD73 antibody | AD2

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Anti Human CD73 Antibody, clone AD2 gallery image 1 — **Published customer image:**
Phycoerythrin conjugated Mouse anti Human CD73 antibody, clone AD2 (**MCA6068PE**) used to evaluate CD73 expression on bone-marrow derived mesenchymal stem cells by flow cytometry
**Image caption:**
Chaturvedi CP, Tripathy NK, Minocha E, Sharma A, Rahman K, Nityanand S.
Altered Expression of Hematopoiesis Regulatory Molecules in Lipopolysaccharide-Induced Bone Marrow Mesenchymal Stem Cells of Patients with Aplastic Anemia. Stem Cells Int. 2018 Oct 17;2018:6901761.

From: Insert article citation with space after colon and Hyperlink to PubMed record.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Mouse anti Human CD73:FITC

Mouse anti Human CD73

Mouse anti Human CD73:RPE

Product Type: Monoclonal Antibody
Clone: AD2
Isotype: IgG1

Product Code	Applications	Pack Size	Your Price	Quantity
MCA6068F Datasheet	F SDS	100 Tests/0.5ml	Log in	Get Quote
MCA6068GA Datasheet	C F IF SDS	0.1 mg	Log in	Get Quote
MCA6068PE Datasheet	F SDS	100 Tests/0.5ml	Log in	Get Quote

Mouse anti Human CD73, clone AD2 recognizes CD73 also known as ecto-5′-nucleotidase. CD73 is a enzyme that catalyzes the coversion of AMP to adenosine. CD73 expression has been detected on subsets of T cells and B cells, follicular dendritic cells, endothelial cells and mesenchymal stem cells. CD73 has been implicated in T cell activation and lymphocyte adhesion.

Product Details

Target Species: Human
Product Form: Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form: Purified IgG - liquid
Product Form: Purified IgG conjugated to R. Phycoerythrin (RPE) - liquid
Preparation: Purified IgG prepared by affinity chromatography
Preparation: Purified IgG prepared by affinity chromatography
Preparation: Purified IgG prepared by affinity chromatography
Buffer Solution: Phosphate buffered saline
Buffer Solution: Phosphate buffered saline
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
0.2% Bovine Serum Albumin
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
0.2% Bovine Serum Albumin
Immunogen: Pre-B leukemia cell line
Approx. Protein Concentrations: IgG concentration 0.5 mg/ml

Storage Information

Storage: Store at +4^oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage: Store at +4^oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee: Guaranteed until date of expiry. Please see product label.
Guarantee: Guaranteed until date of expiry. Please see product label.
Guarantee: Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.

More Information

UniProt: Q6NZX3
Regulatory: For research purposes only

Applications of CD73 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
Flow Cytometry	1/2	1/10
Flow Cytometry	Neat	1/10
Immunofluorescence
Immunohistology - Frozen
Flow Cytometry	Neat

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 10 μl of the suggested working dilution to label 1x10⁶ cells in 100ul
Flow Cytometry: Use 10 μl of the suggested working dilution to label 1x10⁶ cells in 100ul
Flow Cytometry: Use 5μl of the suggested working dilution to label 1x10⁶ cells in 100μl

Secondary Antibodies Available

Description	Product Code	Applications	Pack Size	Your Price	Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	E WB	0.5 mg	Log in	Get Quote
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	F IF	0.1 mg	Log in	Get Quote
Goat anti Mouse IgG (H/L):DyLight®550 (Multi Species Adsorbed)	STAR117D550	F IF WB	0.1 mg	Log in	Get Quote
Goat anti Mouse IgG (H/L):DyLight®650 (Multi Species Adsorbed)	STAR117D650	F IF	0.1 mg	Log in	Get Quote
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	F WB	0.1 mg	Log in	Get Quote
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	F IF WB	0.1 mg	Log in	Get Quote
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	F	0.5 mg	Log in	Get Quote
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	C E WB	0.5 mg	Log in	Get Quote
Goat anti Mouse IgG (Fc):FITC	STAR120F	C F	1 mg	Log in	Get Quote
Goat anti Mouse IgG (Fc):HRP	STAR120P	E WB	1 mg	Log in	Get Quote
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	F	1 ml	Log in	Get Quote
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	C E P RE WB	1 mg	Log in	Get Quote
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	F	0.5 mg	Log in	Get Quote
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	F	1 ml	Log in	Get Quote
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	C E P	0.5 mg	Log in	Get Quote
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	C E WB	1 mg	Log in	Get Quote
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	E	1 mg	Log in	Get Quote
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	F IF WB	0.1 mg	Log in	Get Quote
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	F	1 mg	Log in	Get Quote

Negative Isotype Controls Available

Description	Product Code	Applications	Pack Size	Your Price	Quantity
Mouse IgG1 Negative Control:FITC	MCA928F	F	100 Tests	Log in	Get Quote
Mouse IgG1 Negative Control	MCA928	F	100 Tests	Log in	Get Quote
Mouse IgG1 Negative Control:RPE	MCA928PE	F	100 Tests	Log in	Get Quote

Product Specific References

References for CD73 antibody

Terp, M.G. et al. (2013) Anti-human CD73 monoclonal antibody inhibits metastasis formation in human breast cancer by inducing clustering and internalization of CD73 expressed on the surface of cancer cells.
J Immunol. 191 (8): 4165-73.
Yoo, H.S. et al. (2013) Mesenchymal Stem Cell Lines Isolated by Different Isolation Methods Show Variations in the Regulation of Graft-versus-host Disease.
Immune Netw. 13 (4): 133-40.
Krüger, K.H. et al. (1991) Expression of ecto-5′-nucleotidase (CD73) in normal mammary gland and in breast carcinoma.
Br J Cancer. 63 (1): 114-8.
GarikipatiV, N.S. et al. (2018) Isolation and characterization of mesenchymal stem cells from human fetus heart.
PLoS One. 13 (2): e0192244.
Chaturvedi, C.P. et al. (2018) Altered Expression of Hematopoiesis Regulatory Molecules in Lipopolysaccharide-Induced Bone Marrow Mesenchymal Stem Cells of Patients with Aplastic Anemia.
Stem Cells Int. 2018: 6901761.
Di Paola, A. et al. (2021) Eltrombopag in paediatric immune thrombocytopenia: Iron metabolism modulation in mesenchymal stromal cells.
Br J Haematol. Dec 28 [Epub ahead of print].

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