CD72 antibody | Bu40
Filter by Application:P IP WB Reset
|Mouse anti Human CD72 antibody, clone Bu40 recognizes human CD72, a ~86 kDa cell surface homodimer expressed on B lymphocytes. CD72 is expressed on early B cell precursors, through to mature activated B cells, but is lost upon terminal differentiation to plasma cells.|
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide (NaN3)
- Carrier Free
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Frozen|
|Immunohistology - Paraffin 1||1/50|
- 1This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase. Successful staining has also been accomplished using heat mediated antigen retrieval in citrate buffer pH6.0, see Lorenzi et al. for details
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
References for CD72 antibody
Katira, A. et al. (1992) Occupancy of CD72 (the CD5 counterstructure) enhances interleukin-4-dependent CD23 expression in resting B lymphocytes.
Immunology. 76 (3): 422-6.
Lorenzi, T. et al. (2012) Placental expression of CD100, CD72 and CD45 is dysregulated in human miscarriage.
PLoS One. 7 (5): e35232.
Brown, M.H. & Lacey, E. (2010) A ligand for CD5 is CD5.
J Immunol. 185 (10): 6068-74.
Svensson, H. et al. (1996) The cell surface phenotype of human natural interferon-alpha producing cells as determined by flow cytometry.
Scand J Immunol. 44 (2): 164-72.
Ling, N.R. & Lowe, J.L. (1993) Is the activated T helper stimulus able to induce Ig isotype switching in cultured human B cells?
Int Immunol. 5 (10): 1269-73.
Brzeziêska-błaszczyk, E. & Bąbolewska,E. (2013) Stem cell factor-dependent mast cell proliferation, maturation and activity can be regulated by inhibitory receptors
Centl Eur J of Immunol. 1: 134-40.
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