CD72 antibody | Bu40

Product Code Applications Pack Size List Price Your Price Qty
MCA2501
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F IP P * WB 0.2 mg loader
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loader
MCA2501T
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SDS Safety Datasheet SDS
C F IP P * 25 µg loader
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loader
Search for Batch Specific Datasheets

Mouse anti Human CD72 antibody, clone Bu40 recognizes human CD72, a ~86 kDa cell surface homodimer expressed on B lymphocytes. CD72 is expressed on early B cell precursors, through to mature activated B cells, but is lost upon terminal differentiation to plasma cells.

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Carrier Free
Yes
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/50 1/100
Immunohistology - Frozen
Immunohistology - Paraffin 1 1/50
Immunoprecipitation
Western Blotting
  1. 1This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase. Successful staining has also been accomplished using heat mediated antigen retrieval in citrate buffer pH6.0, see Lorenzi et al. for details
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

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References for CD72 antibody

  1. Katira, A. et al. (1992) Occupancy of CD72 (the CD5 counterstructure) enhances interleukin-4-dependent CD23 expression in resting B lymphocytes.
    Immunology. 76 (3): 422-6.
  2. Lorenzi, T. et al. (2012) Placental expression of CD100, CD72 and CD45 is dysregulated in human miscarriage.
    PLoS One. 7 (5): e35232.
  3. Brown, M.H. & Lacey, E. (2010) A ligand for CD5 is CD5.
    J Immunol. 185 (10): 6068-74.
  4. Svensson, H. et al. (1996) The cell surface phenotype of human natural interferon-alpha producing cells as determined by flow cytometry.
    Scand J Immunol. 44 (2): 164-72.
  5. Ling, N.R. & Lowe, J.L. (1993) Is the activated T helper stimulus able to induce Ig isotype switching in cultured human B cells?
    Int Immunol. 5 (10): 1269-73.
  6. Brzeziêska-błaszczyk, E. & Bąbolewska,E. (2013) Stem cell factor-dependent mast cell proliferation, maturation and activity can be regulated by inhibitory receptors
    Centl Eur J of Immunol. 1: 134-40.

Immunohistology - Paraffin

Western Blotting

RRID
AB_844512
UniProt
P21854
Entrez Gene
CD72
GO Terms
GO:0005102 receptor binding
GO:0007155 cell adhesion
GO:0004888 transmembrane receptor activity
GO:0005887 integral to plasma membrane
GO:0005529 sugar binding
GO:0007411 axon guidance
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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