CD72 antibody | Bu40
Mouse anti Human CD72 antibody, clone Bu40 (MCA2501) used to identify CD72 expressing cells in 1st trimester control placental tissue sections by immunohistochemistry on formalin fixed, paraffin embedded tissue sections following antigen retrieval in citrate buffer pH6.0.
Image caption:
Representative first trimester placenta (control).Paraffin serial sections of representative first trimester placenta (9th week of gestation) from a voluntary termination of pregnancy. A subpopulation of Hofbauer cells, identified by CD68 are positive for CD100. Staining for CD72 (panel c) and CD45 (panel d) is largely negative. Bar = 60μm.
From:
Citation: Lorenzi T, Turi A, Lorenzi M, Paolinelli F, Mancioli F, La Sala L, et al. (2012)
Placental Expression of CD100, CD72 and CD45 Is Dysregulated in Human Miscarriage.
PLoS ONE 7(5): e35232.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human CD72 antibody, clone Bu40 (MCA2501) used to evaluate CD72 expression in miscarriage and normal placental tissue lysates by western blotting following immunopreciptiation with the same antibody.
Image caption:
CD72 protein expression in normal pregnancy and in miscarriage.
Representative immunoblot. MC = miscarriage. 8–9 weeks (8≤ w.g. <9); 9–10 weeks (9≤ w.g. ≤10); >10 weeks (w.g. >10). β-actin: housekeeping protein.
From:
Citation: Lorenzi T, Turi A, Lorenzi M, Paolinelli F, Mancioli F, La Sala L, et al. (2012)
Placental Expression of CD100, CD72 and CD45 Is Dysregulated in Human Miscarriage.
PLoS ONE 7(5): e35232.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human CD72 antibody, clone Bu40 (MCA2501) used to identify CD72 expressing cells in villous stroma following miscarriage by immunohistochemistry on formalin fixed, paraffin embedded tissue sections following antigen retrieval in citrate buffer pH6.0.
Image caption:
Tissue from a representative first miscarriage, 9th week of gestation.
Paraffin serial sections. A subpopulation of Hofbauer cells residing in the villous stroma, are positive for CD72 (red asterisk). Bar = 60μm.
From:
Citation: Lorenzi T, Turi A, Lorenzi M, Paolinelli F, Mancioli F, La Sala L, et al. (2012)
Placental Expression of CD100, CD72 and CD45 Is Dysregulated in Human Miscarriage.
PLoS ONE 7(5): e35232.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human CD72 antibody, clone Bu40 (MCA2501) used to identify CD72 expressing cells in 1st trimester control placental tissue sections by immunohistochemistry on formalin fixed, paraffin embedded tissue sections following antigen retrieval in citrate buffer pH6.0.
Image caption:
Representative first trimester placenta (control).Paraffin serial sections of representative first trimester placenta (9th week of gestation) from a voluntary termination of pregnancy. A subpopulation of Hofbauer cells, identified by CD68 are positive for CD100. Staining for CD72 (panel c) and CD45 (panel d) is largely negative. Bar = 60μm.
From:
Citation: Lorenzi T, Turi A, Lorenzi M, Paolinelli F, Mancioli F, La Sala L, et al. (2012)
Placental Expression of CD100, CD72 and CD45 Is Dysregulated in Human Miscarriage.
PLoS ONE 7(5): e35232.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human CD72
- Product Type
- Monoclonal Antibody
- Clone
- Bu40
- Isotype
- IgG2a
- Specificity
- CD72
Antibody Quality and Performance Guarantee
| Mouse anti Human CD72 antibody, clone Bu40 recognizes human CD72, a ~86 kDa cell surface homodimer expressed on B lymphocytes. CD72 is expressed on early B cell precursors, through to mature activated B cells, but is lost upon terminal differentiation to plasma cells. |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide (NaN3) - Carrier Free
- Yes
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/50 | 1/100 |
| Immunohistology - Frozen | |
||
| Immunohistology - Paraffin 1 | |
1/50 | |
| Immunoprecipitation | |
||
| Western Blotting | |
- 1This product requires protein digestion pre-treatment of paraffin sections e.g. trypsin or pronase. Successful staining has also been accomplished using heat mediated antigen retrieval in citrate buffer pH6.0, see Lorenzi et al. for details
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG2a Negative Control | MCA929 | F | 100 Tests |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG2a Negative Control | ||||||
References for CD72 antibody
-
Katira, A. et al. (1992) Occupancy of CD72 (the CD5 counterstructure) enhances interleukin-4-dependent CD23 expression in resting B lymphocytes.
Immunology. 76 (3): 422-6. -
Ling, N.R. & Lowe, J.L. (1993) Is the activated T helper stimulus able to induce Ig isotype switching in cultured human B cells?
Int Immunol. 5 (10): 1269-73. -
Svensson, H. et al. (1996) The cell surface phenotype of human natural interferon-alpha producing cells as determined by flow cytometry.
Scand J Immunol. 44 (2): 164-72. -
Brown, M.H. & Lacey, E. (2010) A ligand for CD5 is CD5.
J Immunol. 185 (10): 6068-74. -
Lorenzi, T. et al. (2012) Placental expression of CD100, CD72 and CD45 is dysregulated in human miscarriage.
PLoS One. 7 (5): e35232. -
Brzeziêska-błaszczyk, E. & Bąbolewska,E. (2013) Stem cell factor-dependent mast cell proliferation, maturation and activity can be regulated by inhibitory receptors
Centl Eur J of Immunol. 1: 134-40.
- RRID
- AB_844512
- UniProt
- P21854
- Entrez Gene
- CD72
- GO Terms
- GO:0005102 receptor binding
- GO:0007155 cell adhesion
- GO:0004888 transmembrane receptor activity
- GO:0005887 integral to plasma membrane
- GO:0005529 sugar binding
- GO:0007411 axon guidance
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