CD71 antibody | DF1513
Mouse anti Human CD71 antibody, clone DF1513 (MCA1148) used to evaluate CD71 expression on erythroid cells during differentiation by flow cytometry.
Image caption:
Level of RBC membrane proteins in BEL-A compared to normal adult erythroid cells during erythropoiesis.
BEL-A cells in expansion medium (undif), and at day 4 and 10 following transfer to differentiation media, and erythroblasts differentiated from adult peripheral blood CD34+ cells at day 5, 12 and 21 in culture were incubated with antibodies to the proteins indicated and analysed by Flow Cytometry. Histograms illustrating fluorescent intensity of BEL-A cells incubated with antibodies to indicated proteins at labelled stages of differentiation. Percentages refer to percentage positivity.
From: Trakarnsanga K, Griffiths RE, Wilson MC, Blair A, Satchwell TJ, Meinders M, Cogan N, Kupzig S, Kurita R, Nakamura Y, Toye AM, Anstee DJ, Frayne J.
An immortalized adult human erythroid line facilitates sustainable and scalable generation of functional red cells.
Nat Commun. 2017 Mar 14;8:14750.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Filter by Application:
F ResetMouse anti Human CD71
- Product Type
- Monoclonal Antibody
- Clone
- DF1513
- Isotype
- IgG1
- Specificity
- CD71
Antibody Quality and Performance Guarantee
| Mouse anti Human CD71 antibody, clone DF1513 recognizes the human CD71 cell surface antigen, a ~190 kDa homodimeric glycoprotein expressed by proliferating cells. CD71 is also known as the transferrin receptor. Mutation of the TFRC gene has been implicated in the development of Immunodeficiency 46 (IMD46) a combined immunodeficiency characterized by early onset chronic diarrhea, recurrent infections and intermittent neutropenia and thrombocytopenia (Jabara et al. 2016) |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rhesus Monkey Mustelid Ferret - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- KGI cell line.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the mouse NSI myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/10 | 1/25 |
| Immunofluorescence | |
||
| Immunohistology - Frozen | |||
| Immunohistology - Paraffin |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control | ||||||
References for CD71 antibody
-
Sopper, S. et al. (1997) Lymphocyte subsets and expression of differentiation markers in blood and lymphoid organs of rhesus monkeys.
Cytometry. 29 (4): 351-62. -
Martel, C.J. & Aasted, B. (2009) Characterization of antibodies against ferret immunoglobulins, cytokines and CD markers.
Vet Immunol Immunopathol. 132:109-15. -
Meng, J. et al. (2011) Contribution of human muscle-derived cells to skeletal muscle regeneration in dystrophic host mice.
PLoS One. 6(3):e17454. -
Stockwin, L.H. et al. (2009) Artemisinin dimer anticancer activity correlates with heme-catalyzed reactive oxygen species generation and endoplasmic reticulum stress induction.
Int J Cancer. 125: 1266-75. -
Janes, P.W. et al. (1999) Aggregation of lipid rafts accompanies signaling via the T cell antigen receptor.
J Cell Biol. 147: 447-61. -
Makoveichuk, E. et al. (2012) Inactivation of lipoprotein lipase occurs on the surface of THP-1 macrophages where oligomers of angiopoietin-like protein 4 are formed.
Biochem Biophys Res Commun. 425:138-43. -
Procaccini, C. et al. (2012) Leptin-induced mTOR activation defines a specific molecular and transcriptional signature controlling CD4+ effector T cell responses.
J Immunol. 189: 2941-53. -
Weissgerber, P. et al. (2003) Investigation of mechanisms involved in phagocytosis of Legionella pneumophila by human cells.
FEMS Microbiol Lett. 219 (2): 173-9.
View The Latest Product References
-
Trakarnsanga, K. et al. (2017) An immortalized adult human erythroid line facilitates sustainable and scalable generation of functional red cells.
Nat Commun. 8: 14750.
- Synonyms
- Transferrin Receptor
- RRID
- AB_1125283
- UniProt
- P02786
- Entrez Gene
- TFRC
- GO Terms
- GO:0008233 peptidase activity
- GO:0004998 transferrin receptor activity
- GO:0005887 integral to plasma membrane
- GO:0005768 endosome
- GO:0005905 coated pit
- GO:0006508 proteolysis
- GO:0006879 cellular iron ion homeostasis
- GO:0006897 endocytosis
- GO:0033572 transferrin transport
- View More GO Terms
- GO:0042470 melanosome
- GO:0044419 interspecies interaction between organisms
- GO:0055085 transmembrane transport
View more products with CD71 specificity
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