CD71 antibody | DF1513
Mouse anti Human CD71 antibody, clone DF1513 (MCA1148) used to evaluate CD71 expression on erythroid cells during differentiation by flow cytometry.
Image caption:
Level of RBC membrane proteins in BEL-A compared to normal adult erythroid cells during erythropoiesis.
BEL-A cells in expansion medium (undif), and at day 4 and 10 following transfer to differentiation media, and erythroblasts differentiated from adult peripheral blood CD34+ cells at day 5, 12 and 21 in culture were incubated with antibodies to the proteins indicated and analysed by Flow Cytometry. Histograms illustrating fluorescent intensity of BEL-A cells incubated with antibodies to indicated proteins at labelled stages of differentiation. Percentages refer to percentage positivity.
From: Trakarnsanga K, Griffiths RE, Wilson MC, Blair A, Satchwell TJ, Meinders M, Cogan N, Kupzig S, Kurita R, Nakamura Y, Toye AM, Anstee DJ, Frayne J.
An immortalized adult human erythroid line facilitates sustainable and scalable generation of functional red cells.
Nat Commun. 2017 Mar 14;8:14750.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Human CD71:FITC
Mouse anti Human CD71:Low Endotoxin
Mouse anti Human CD71
Mouse anti Human CD71:RPE
- Product Type
- Monoclonal Antibody
- Clone
- DF1513
- Isotype
- IgG1
Mouse anti Human CD71 antibody, clone DF1513 recognizes the human CD71 cell surface antigen, a ~190 kDa homodimeric glycoprotein expressed by proliferating cells. CD71 is also known as the transferrin receptor. Mutation of the TFRC gene has been implicated in the development of Immunodeficiency 46 (IMD46) a combined immunodeficiency characterized by early onset chronic diarrhea, recurrent infections and intermittent neutropenia and thrombocytopenia (Jabara et al. 2016)
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rhesus Monkey Mustelid Ferret - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Reconstitution
- Pack Size: 25 TestsReconstitute in 0.25 ml disilled waterPack Size: 100 TestsReconstitute with 1 ml distilled water
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Preservative Stabilisers
- None present
- Preservative Stabilisers
0.09% Sodium Azide - Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin 5% Sucrose - Carrier Free
- Yes
- Carrier Free
- Yes
- Immunogen
- KGI cell line.
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the mouse NSI myeloma cell line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Prior to reconstitution store at +4oC. Following reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 12 months from date of despatch.
- Shelf Life
- 18 months from date of despatch.
- Shelf Life
- 12 months from date of reconstitution.
More Information
- UniProt
- P02786 Related reagents
- Entrez Gene
- TFRC Related reagents
- GO Terms
- GO:0008233 peptidase activity
- GO:0004998 transferrin receptor activity
- GO:0005887 integral to plasma membrane
- GO:0005768 endosome
- GO:0005905 coated pit
- GO:0006508 proteolysis
- GO:0006879 cellular iron ion homeostasis
- GO:0006897 endocytosis
- GO:0033572 transferrin transport
- GO:0042470 melanosome
- GO:0044419 interspecies interaction between organisms
- GO:0055085 transmembrane transport
- Regulatory
- For research purposes only
Applications of CD71 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | Neat | ||
| Flow Cytometry | 1/10 | 1/25 | |
| Immunofluorescence | |||
| Immunohistology - Frozen | |||
| Immunohistology - Paraffin | |||
| Flow Cytometry | 1/10 | 1/25 | |
| Immunofluorescence | |||
| Immunohistology - Frozen | |||
| Immunohistology - Paraffin | |||
| Flow Cytometry | Neat |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the diluted antibody to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control:FITC | MCA928F | 100 Tests | F | ||
| Mouse IgG1 Negative Control:Low Endotoxin | MCA928EL | 0.5 mg | C F | ||
| Mouse IgG1 Negative Control | MCA928 | 100 Tests | F | ||
| Mouse IgG1 Negative Control:RPE | MCA928PE | 100 Tests | F |
Useful Reagents Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Human Seroblock | BUF070A | 50 Test | F | ||
| Human Seroblock | BUF070B | 200 Test | F | ||
| Human Seroblock | BUF070A | 50 Test | F | ||
| Human Seroblock | BUF070B | 200 Test | F |
Product Specific References
References for CD71 antibody
-
Sopper, S. et al. (1997) Lymphocyte subsets and expression of differentiation markers in blood and lymphoid organs of rhesus monkeys.
Cytometry. 29 (4): 351-62. -
Martel, C.J. & Aasted, B. (2009) Characterization of antibodies against ferret immunoglobulins, cytokines and CD markers.
Vet Immunol Immunopathol. 132:109-15. -
Meng, J. et al. (2011) Contribution of human muscle-derived cells to skeletal muscle regeneration in dystrophic host mice.
PLoS One. 6(3):e17454. -
Stockwin, L.H. et al. (2009) Artemisinin dimer anticancer activity correlates with heme-catalyzed reactive oxygen species generation and endoplasmic reticulum stress induction.
Int J Cancer. 125: 1266-75. -
Janes, P.W. et al. (1999) Aggregation of lipid rafts accompanies signaling via the T cell antigen receptor.
J Cell Biol. 147: 447-61. -
Makoveichuk, E. et al. (2012) Inactivation of lipoprotein lipase occurs on the surface of THP-1 macrophages where oligomers of angiopoietin-like protein 4 are formed.
Biochem Biophys Res Commun. 425:138-43. -
Procaccini, C. et al. (2012) Leptin-induced mTOR activation defines a specific molecular and transcriptional signature controlling CD4+ effector T cell responses.
J Immunol. 189: 2941-53. -
Weissgerber, P. et al. (2003) Investigation of mechanisms involved in phagocytosis of Legionella pneumophila by human cells.
FEMS Microbiol Lett. 219 (2): 173-9. -
Trakarnsanga, K. et al. (2017) An immortalized adult human erythroid line facilitates sustainable and scalable generation of functional red cells.
Nat Commun. 8: 14750.
