CD70 antibody | Bu69
Mouse anti Human CD70 antibody, clone Bu69 recognises human CD70, also known as CD27 ligand (CD27-L) or Tumor necrosis factor ligand superfamily member 7. CD70 is a 193 amino acid ~29 kDa single pass type II transmembrane glycoprotein belonging to the tumour necrosis factor (TNF) family (UniProt: P32970).
CD70 is expressed on a small number of activated T-cells and the majority of activated B-cells (Bowman et al. 1994). Interaction of CD70 with its receptor, CD27, is thought to play an important role in lymphocyte activation, proliferation, survival and differentiation (Hintzen et al. 1994).
Mouse anti Human CD70 antibody, clone Bu69 has been used for the inhibition of neural stem cell induced apoptosis of allogeneic T cells in vitro (Lee et al. 2013).
CD70 is expressed on a small number of activated T-cells and the majority of activated B-cells (Bowman et al. 1994). Interaction of CD70 with its receptor, CD27, is thought to play an important role in lymphocyte activation, proliferation, survival and differentiation (Hintzen et al. 1994).
Mouse anti Human CD70 antibody, clone Bu69 has been used for the inhibition of neural stem cell induced apoptosis of allogeneic T cells in vitro (Lee et al. 2013).
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P32970
- Entrez Gene
- CD70
- GO Terms
- GO:0000299 integral to membrane of membrane fraction
- GO:0007165 signal transduction
- GO:0005887 integral to plasma membrane
- GO:0005125 cytokine activity
- GO:0005164 tumor necrosis factor receptor binding
- GO:0005615 extracellular space
- GO:0006917 induction of apoptosis
- GO:0006955 immune response
- GO:0007267 cell-cell signaling
- GO:0008283 cell proliferation
- Regulatory
- For research purposes only
Applications of CD70 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | 1/50 | 1/200 | |
| Immunohistology - Frozen |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
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Secondary Antibodies Available
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
|
Product Specific References
References for CD70 antibody
-
Garland Publishing. (1997) Leucocyte typing VI. White cell differentiation antigens.
p522 - 4 -
Zambello, R. et al. (2000) Analysis of TNF-receptor and ligand superfamily molecules in patients with lymphoproliferative disease of granular lymphocytes.
Blood. 96 (2): 647-54. -
Lee, E.M. et al. (2013) CD70-CD27 ligation between neural stem cells and CD4+ T cells induces Fas-FasL-mediated T-cell death.
Stem Cell Res Ther. 4 (3): 56.
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