CD70 antibody | Bu69

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Mouse anti Human CD70

Product Type
Monoclonal Antibody
Clone
Bu69
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA1765GT C F 25 µg
MCA1765G C F 0.2 mg
Mouse anti Human CD70 antibody, clone Bu69 recognises human CD70, also known as CD27 ligand (CD27-L) or Tumor necrosis factor ligand superfamily member 7. CD70 is a 193 amino acid ~29 kDa single pass type II transmembrane glycoprotein belonging to the tumour necrosis factor (TNF) family (UniProt: P32970).

CD70 is expressed on a small number of activated T-cells and the majority of activated B-cells (Bowman et al. 1994). Interaction of CD70 with its receptor, CD27, is thought to play an important role in lymphocyte activation, proliferation, survival and differentiation (Hintzen et al. 1994).

Mouse anti Human CD70 antibody, clone Bu69 has been used for the inhibition of neural stem cell induced apoptosis of allogeneic T cells in vitro (Lee et al. 2013).

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P32970 Related reagents
Entrez Gene
CD70 Related reagents
GO Terms
GO:0000299 integral to membrane of membrane fraction
GO:0007165 signal transduction
GO:0005887 integral to plasma membrane
GO:0005125 cytokine activity
GO:0005164 tumor necrosis factor receptor binding
GO:0005615 extracellular space
GO:0006917 induction of apoptosis
GO:0006955 immune response
GO:0007267 cell-cell signaling
GO:0008283 cell proliferation
Regulatory
For research purposes only

Applications of CD70 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/50 1/200
Immunohistology - Frozen
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG1 Negative Control MCA928 100 Tests F

Application Based External Images

Functional Assays

Product Specific References

References for CD70 antibody

  1. Garland Publishing. (1997) Leucocyte typing VI. White cell differentiation antigens.
    p522 - 4
  2. Zambello, R. et al. (2000) Analysis of TNF-receptor and ligand superfamily molecules in patients with lymphoproliferative disease of granular lymphocytes.
    Blood. 96 (2): 647-54.
  3. Lee, E.M. et al. (2013) CD70-CD27 ligation between neural stem cells and CD4+ T cells induces Fas-FasL-mediated T-cell death.
    Stem Cell Res Ther. 4 (3): 56.