CD66e antibody | CB30

Mouse anti Human CD66e

Product Type
Monoclonal Antibody
Clone
CB30
Isotype
IgG1
Specificity
CD66e

Product Code Applications Pack Size List Price Your Price Qty
1820-4700
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F IP P * 0.2 mg loader
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loader
1820-4700T
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F IP P * 25 µg loader
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loader
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Mouse anti Human CD66e antibody, clone CB30 recognizes human CD66e, also known as carcinoembryonic antigen (CEA).

CD66e is a member of the immunoglobulin supergene family that is mainly expressed on epithelial cells. CD66e may play a role in cancer cells metastasis, and has been used clinically as a tumor marker for early detection of disease.

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Human carcinoembryonic antigen.
Approx. Protein Concentrations
1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Immunohistology - Frozen
Immunohistology - Paraffin 1 1/100 1/200
Immunoprecipitation
  1. 1This product requires protein digestion pre-treatment of paraffin sections e.g. pronase.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Adenocarcinoma of colon.

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References for CD66e antibody

  1. Horenstein, A.L. et al. (2003) Design and scaleup of downstream processing of monoclonal antibodies for cancer therapy: from research to clinical proof of principle.
    J Immunol Methods. 275 (1-2): 99-112.
  2. Manini, I. et al. (2020) Heterogeneity Matters: Different Regions of Glioblastoma Are Characterized by Distinctive Tumor-Supporting Pathways.
    Cancers (Basel). 12 (10)Oct 13 [Epub ahead of print].

Further Reading

  1. Thompson, J.A. et al. (1991) Carcinoembryonic antigen gene family: molecular biology and clinical perspectives.
    J Clin Lab Anal. 5 (5): 344-66.

Synonyms
CEA
RRID
AB_620088
UniProt
P06731
Entrez Gene
CEACAM5
GO Terms
GO:0005887 integral to plasma membrane
GO:0031225 anchored to membrane
GO:0016323 basolateral plasma membrane
View more products with CD66 specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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