CD66b antibody | rA10B10-04/4H2
Mouse anti Human CD66b
- Product Type
- Monoclonal Antibody
- Clone
- rA10B10-04/4H2
- Isotype
- IgG1
- Specificity
- CD66b
Antibody Quality and Performance Guarantee
TZA080GA
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| Mouse anti Human CD66b antibody, clone rA10B10-04/4H2, is a recombinant monoclonal antibody that recognizes CD66b, also known as cell adhesion molecule CEACAM8, CGM6 or NCA-95. CD66b is a member of the carcinoembryonic antigen (CEA) family, which is involved in a range of cellular processes through homotypic and heterotypic interactions. CD66b consists of an N-terminal region homologous to the immunoglobulin variable domain, two domains homologous to the immunoglobulin constant domain and is linked to the membrane via a GPI anchor. CD66b is highly expressed in eosinophils and neutrophils, and weakly in bone myelocytes. CD66b is involved in cell adhesion in a calcium-independent manner, functioning with other cell adhesion molecules such as CD66c. CD66b is expressed on leukocytes of chronic myeloid leukemia patients. The level of CD66b-positive neutrophils found within tumors of patients with resectable non-small cell lung cancer (NSCLC) has been found to correlate with the risk of disease recurrence. CD66b+ granulocytes were significantly higher in patients with severe COVID-19 compared to either asymptomatic or convalescent patients. CD66b crosslinking leads to the directed release of preformed IL-8 from neutrophils but does not induce do novo synthesis of cytokines. Cross-linking the CD66b with a specific mAb or a ligand, galectin-3, induces tight cellular adhesion, superoxide generation, and degranulation. |
- Target Species
- Human
- Product Form
- Purified recombinant IgG containing a SpyTag at the C-terminus of the antibody heavy chain – liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Source
- CHO cells
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Recombinant CD66b Gln35-Asp320 expressed by mammalian cells
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 5μl of the suggested working dilution to label 0.5x106 cells in 100μl. Best practices suggest a 5 min centrifugation at 6,000g prior to sample application.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control | ||||||
- Synonyms
- CD66
- CEACAM8
- UniProt
- P31997
- Entrez Gene
- CEACAM8
- GO Terms
- GO:0005887 integral to plasma membrane
- GO:0005615 extracellular space
- GO:0006955 immune response
- GO:0031225 anchored to membrane
TZA080GA
64744246
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