CD64 antibody | 10.1
Mouse anti Human CD64 antibody, clone 10.1 (MCA756G) used as a blocking agent to assess the effect of FcγRs on heparin binding protein (HBP) release by ELISA.
Image caption:
Antibodies against FcγRII inhibit HBP release.
Whole blood (diluted 1∶10) was incubated with antibodies against different FcγRs, isotype control antibodies, or buffer alone, for 20 minutes at 37°C. M1 protein was added and the HBP release was measured. Data are expressed as percentage of release without any antibodies added, and are the mean±SD from three different individuals, each done in duplicate. The different treatment groups were compared using ANOVA, and when found significantly different the treatment groups were compared against the control group using Dunnett's 2 sided test.
From: Kahn F, Mörgelin M, Shannon O, Norrby-Teglund A, Herwald H, Olin AI, et al. (2008) Antibodies against a Surface Protein of Streptococcus pyogenes Promote a Pathological Inflammatory Response. PLoS Pathog 4(9): e1000149. .
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Rat anti Mouse CD200 antibody, clone OX-90 (MCA1958) used to evaluate CD200 expression in mouse intestine by immunofluorescence.
Image caption:
CD200 expression within the intestine.
Immunofluorescence staining for CD200 (green) in colon tissue from untreated mice. Cell nuclei were visualized by DAPI (blue). Intense CD200 immunostaining was observed in the subepithelial layer of the colonic villi (magnification 20×), scale bar = 100 μm;
From: Tonecka, K.; Braniewska, A.; Pilch, Z.; Sas, Z.; Skorzynski, M.; Manuali, E.; Rygiel, T.P.
The CD200 Regulates Inflammation in Mice Independently of TNF-α Production.
Int. J. Mol. Sci. 2021, 22, 5358.
doi: 10.3390/ijms22105358
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Rat anti Mouse CD200 antibody, clone OX-90 (MCA1958) used to evaluate CD200 expression in mouse intestine by immunofluorescence.
Image caption:
CD200 expression within the intestine.
Immunofluorescence staining for CD200 (green) in colon tissue from untreated mice. Cell nuclei were visualized by DAPI (blue). Intense CD200 immunostaining was observed in the subepithelial layer of the colonic villi (magnification 40×), scale bar = 100 μm;
From: Tonecka, K.; Braniewska, A.; Pilch, Z.; Sas, Z.; Skorzynski, M.; Manuali, E.; Rygiel, T.P.
The CD200 Regulates Inflammation in Mice Independently of TNF-α Production.
Int. J. Mol. Sci. 2021, 22, 5358.
doi: 10.3390/ijms22105358
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Mouse anti Human CD64
- Product Type
- Monoclonal Antibody
- Clone
- 10.1
- Isotype
- IgG1
- Specificity
- CD64
Antibody Quality and Performance Guarantee
| Mouse anti Human CD64 antibody, clone 10.1 recognizes the human CD64 cell surface antigen, a ~75 kDa glycoprotein expressed by monocytes. The antigen acts as a high affinity receptor for human IgG, and is also known as FcRI. Mouse anti Human CD64 antibody, clone 10.1 blocks binding of immunoglobulin to FcRI. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Baboon Cynomolgus monkey Rhesus Monkey - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- MCA756G: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- MCA756GT: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Carrier Free
- Yes
- Immunogen
- Human monocytes
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0-Ag14 myeloma cell line
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/50 | 1/100 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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Log in | ||
| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control | ||||||
Source Reference
-
Dougherty, G.J. et al. (1987) The human mononuclear phagocyte high-affinity Fc receptor, FcRI, defined by a monoclonal antibody, 10.1.
Eur J Immunol. 17 (10): 1453-9.
References for CD64 antibody
-
Scheinecker, C. et al. (1998) Initiation of the autologous mixed lymphocyte reaction requires the expression of costimulatory molecules B7-1 and B7-2 on human peripheral blood dendritic cells.
J Immunol. 161: 3966-73. -
Fadlon,. E. et al. (1998) Blood polymorphonuclear leukocytes from the majority of sickle cell patients in the crisis phase of the disease show enhanced adhesion to vascular endothelium and increased expression of CD64.
Blood. 91: 266-74. -
Navarro-López, F. et al. (2003) Late T-lymphocyte and monocyte activation in coronary restenosis. Evidence for a persistent inflammatory/immune mechanism?
Rev Esp Cardiol. 56: 465-72. -
Beekman, J.M. et al. (2004) Direct interaction between FcgammaRI (CD64) and periplakin controls receptor endocytosis and ligand binding capacity.
Proc Natl Acad Sci U S A.101: 10392-7. -
Kahn, F. et al. (2008) Antibodies against a surface protein of
Streptococcus pyogenes promote a pathological inflammatory response.
PLoS Pathog. 4 (9): e1000149. -
Wagner, C. et al. (2008) T lymphocytes in acute bacterial infection: increased prevalence of CD11b(+) cells in the peripheral blood and recruitment to the infected site.
Immunology. 125: 503-9. -
Eisenhardt, S.U. et al. (2009) Dissociation of pentameric to monomeric C-reactive protein on activated platelets localizes inflammation to atherosclerotic plaques.
Circ Res. 105: 128-37. -
Tanaka, M. et al. (2009) Activation of Fc gamma RI on monocytes triggers differentiation into immature dendritic cells that induce autoreactive T cell responses.
J Immunol. 183: 2349-55.
View The Latest Product References
-
Liu M et al. (2011) Vitellogenin mediates phagocytosis through interaction with FcγR.
Mol Immunol. 49 (1-2): 211-8. -
Fet, N.G. et al. (2012) Reduction of activated macrophages after ischaemia-reperfusion injury diminishes oxidative stress and ameliorates renal damage.
Nephrol Dial Transplant. 27 (8): 3149-55. -
Kapelski S et al. (2014) Assessment of the neutrophilic antibody-dependent respiratory burst (ADRB) response to Plasmodium falciparum.
J Leukoc Biol. 96 (6): 1131-42. -
Hristodorov, D. et al. (2016) Fully human MAP-fusion protein selectively targets and eliminates proliferating CD64(+) M1 macrophages.
Immunol Cell Biol. 94 (5): 470-8. -
Loi, A.L.T. et al. (2017) Proteomic profiling of peripheral blood neutrophils identifies two inflammatory phenotypes in stable COPD patients.
Respir Res. 18 (1): 100. -
Rahabi, M. et al. (2020) Divergent Roles for Macrophage C-type Lectin Receptors, Dectin-1 and Mannose Receptors, in the Intestinal Inflammatory Response.
Cell Rep. 30 (13): 4386-98.e5. -
Tonecka, K. et al. (2021) The CD200 Regulates Inflammation in Mice Independently of TNF-α Production.
Int J Mol Sci. 22 (10): 5358.
- Synonyms
- FcRI
- RRID
- AB_322931
- UniProt
- P12314
- Entrez Gene
- FCGR1A
- GO Terms
- GO:0005886 plasma membrane
- GO:0016021 integral to membrane
- GO:0004872 receptor activity
- GO:0005057 receptor signaling protein activity
- GO:0006911 phagocytosis, engulfment
- GO:0019864 IgG binding
- GO:0045087 innate immune response
- GO:0060333 interferon-gamma-mediated signaling pathway
MCA756G
150154
163389
1706
MCA756GT
151905
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