CD64 antibody | 10.1

Product Code Applications Pack Size List Price Your Price Qty
MCA756G
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.2 mg loader
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loader
MCA756GT
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 25 µg loader
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loader
Search for Batch Specific Datasheets

Mouse anti Human CD64 antibody, clone 10.1 recognizes the human CD64 cell surface antigen, a ~75 kDa glycoprotein expressed by monocytes. The antigen acts as a high affinity receptor for human IgG, and is also known as FcRI.

Mouse anti Human CD64 antibody, clone 10.1 blocks binding of immunoglobulin to FcRI.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Baboon
Cynomolgus monkey
Rhesus Monkey
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
MCA756G: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
MCA756GT: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Human monocytes
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0-Ag14 myeloma cell line
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/50 1/100
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

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References for CD64 antibody

  1. Dougherty, G.J. et al. (1987) The human mononuclear phagocyte high-affinity Fc receptor, FcRI, defined by a monoclonal antibody, 10.1.
    Eur J Immunol. 17 (10): 1453-9.
  2. Beekman, J.M. et al. (2004) Direct interaction between FcgammaRI (CD64) and periplakin controls receptor endocytosis and ligand binding capacity.
    Proc Natl Acad Sci U S A.101: 10392-7.
  3. Tanaka, M. et al. (2009) Activation of Fc gamma RI on monocytes triggers differentiation into immature dendritic cells that induce autoreactive T cell responses.
    J Immunol. 183: 2349-55.
  4. Fet, N.G. et al. (2012) Reduction of activated macrophages after ischaemia-reperfusion injury diminishes oxidative stress and ameliorates renal damage.
    Nephrol Dial Transplant. 27 (8): 3149-55.
  5. Wagner, C. et al. (2008) T lymphocytes in acute bacterial infection: increased prevalence of CD11b(+) cells in the peripheral blood and recruitment to the infected site.
    Immunology. 125: 503-9.
  6. Eisenhardt, S.U. et al. (2009) Dissociation of pentameric to monomeric C-reactive protein on activated platelets localizes inflammation to atherosclerotic plaques.
    Circ Res. 105: 128-37.
  7. Fadlon,. E. et al. (1998) Blood polymorphonuclear leukocytes from the majority of sickle cell patients in the crisis phase of the disease show enhanced adhesion to vascular endothelium and increased expression of CD64.
    Blood. 91: 266-74.
  8. Scheinecker, C. et al. (1998) Initiation of the autologous mixed lymphocyte reaction requires the expression of costimulatory molecules B7-1 and B7-2 on human peripheral blood dendritic cells.
    J Immunol. 161: 3966-73.
  9. View The Latest Product References
  10. Navarro-López, F. et al. (2003) Late T-lymphocyte and monocyte activation in coronary restenosis. Evidence for a persistent inflammatory/immune mechanism?
    Rev Esp Cardiol. 56: 465-72.
  11. Liu M et al. (2011) Vitellogenin mediates phagocytosis through interaction with FcγR.
    Mol Immunol. 49 (1-2): 211-8.
  12. Kapelski S et al. (2014) Assessment of the neutrophilic antibody-dependent respiratory burst (ADRB) response to Plasmodium falciparum.
    J Leukoc Biol. 96 (6): 1131-42.
  13. Loi, A.L.T. et al. (2017) Proteomic profiling of peripheral blood neutrophils identifies two inflammatory phenotypes in stable COPD patients.
    Respir Res. 18 (1): 100.
  14. Hristodorov, D. et al. (2016) Fully human MAP-fusion protein selectively targets and eliminates proliferating CD64(+) M1 macrophages.
    Immunol Cell Biol. 94 (5): 470-8.
  15. Kahn, F. et al. (2008) Antibodies against a surface protein of
    Streptococcus pyogenes promote a pathological inflammatory response.
    PLoS Pathog. 4 (9): e1000149.

Further Reading

  1. Yoshino, N. et al. (2000) Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of Cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies.
    Exp Anim. 49 (2): 97-110.

Flow Cytometry

Functional Assays

Immunofluorescence

Synonyms
FcRI
RRID
AB_322931
UniProt
P12314
Entrez Gene
FCGR1A
GO Terms
GO:0005886 plasma membrane
GO:0016021 integral to membrane
GO:0004872 receptor activity
GO:0005057 receptor signaling protein activity
GO:0006911 phagocytosis, engulfment
GO:0019864 IgG binding
GO:0045087 innate immune response
GO:0060333 interferon-gamma-mediated signaling pathway
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