CD64 antibody | 10.1
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Mouse anti Human CD64
- Product Type
- Monoclonal Antibody
|Mouse anti Human CD64 antibody, clone 10.1 recognizes the human CD64 cell surface antigen, a ~75 kDa glycoprotein expressed by monocytes. The antigen acts as a high affinity receptor for human IgG, and is also known as FcRI.
Mouse anti Human CD64 antibody, clone 10.1 blocks binding of immunoglobulin to FcRI.
- Target Species
- Species Cross-Reactivity
Target Species Cross Reactivity Baboon Cynomolgus monkey Rhesus Monkey
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- MCA756G: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- MCA756GT: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide
- Carrier Free
- Human monocytes
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0-Ag14 myeloma cell line
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
|Application Name||Verified||Min Dilution||Max Dilution|
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Dougherty, G.J. et al. (1987) The human mononuclear phagocyte high-affinity Fc receptor, FcRI, defined by a monoclonal antibody, 10.1.
Eur J Immunol. 17 (10): 1453-9.
References for CD64 antibody
Scheinecker, C. et al. (1998) Initiation of the autologous mixed lymphocyte reaction requires the expression of costimulatory molecules B7-1 and B7-2 on human peripheral blood dendritic cells.
J Immunol. 161: 3966-73.
Fadlon,. E. et al. (1998) Blood polymorphonuclear leukocytes from the majority of sickle cell patients in the crisis phase of the disease show enhanced adhesion to vascular endothelium and increased expression of CD64.
Blood. 91: 266-74.
Navarro-López, F. et al. (2003) Late T-lymphocyte and monocyte activation in coronary restenosis. Evidence for a persistent inflammatory/immune mechanism?
Rev Esp Cardiol. 56: 465-72.
Beekman, J.M. et al. (2004) Direct interaction between FcgammaRI (CD64) and periplakin controls receptor endocytosis and ligand binding capacity.
Proc Natl Acad Sci U S A.101: 10392-7.
Kahn, F. et al. (2008) Antibodies against a surface protein of
Streptococcus pyogenes promote a pathological inflammatory response.
PLoS Pathog. 4 (9): e1000149.
Wagner, C. et al. (2008) T lymphocytes in acute bacterial infection: increased prevalence of CD11b(+) cells in the peripheral blood and recruitment to the infected site.
Immunology. 125: 503-9.
Eisenhardt, S.U. et al. (2009) Dissociation of pentameric to monomeric C-reactive protein on activated platelets localizes inflammation to atherosclerotic plaques.
Circ Res. 105: 128-37.
Tanaka, M. et al. (2009) Activation of Fc gamma RI on monocytes triggers differentiation into immature dendritic cells that induce autoreactive T cell responses.
J Immunol. 183: 2349-55.
View The Latest Product References
Liu M et al. (2011) Vitellogenin mediates phagocytosis through interaction with FcγR.
Mol Immunol. 49 (1-2): 211-8.
Fet, N.G. et al. (2012) Reduction of activated macrophages after ischaemia-reperfusion injury diminishes oxidative stress and ameliorates renal damage.
Nephrol Dial Transplant. 27 (8): 3149-55.
Kapelski S et al. (2014) Assessment of the neutrophilic antibody-dependent respiratory burst (ADRB) response to Plasmodium falciparum.
J Leukoc Biol. 96 (6): 1131-42.
Hristodorov, D. et al. (2016) Fully human MAP-fusion protein selectively targets and eliminates proliferating CD64(+) M1 macrophages.
Immunol Cell Biol. 94 (5): 470-8.
Loi, A.L.T. et al. (2017) Proteomic profiling of peripheral blood neutrophils identifies two inflammatory phenotypes in stable COPD patients.
Respir Res. 18 (1): 100.
- Entrez Gene
- GO Terms
- GO:0005886 plasma membrane
- GO:0016021 integral to membrane
- GO:0004872 receptor activity
- GO:0005057 receptor signaling protein activity
- GO:0006911 phagocytosis, engulfment
- GO:0019864 IgG binding
- GO:0045087 innate immune response
- GO:0060333 interferon-gamma-mediated signaling pathway
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