CD63 antibody | MEM-259

Mouse anti Human CD63

Product Type
Monoclonal Antibody
Clone
MEM-259
Isotype
IgG1
Specificity
CD63

Product Code Applications Pack Size List Price Your Price Qty
MCA2142T
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F IF IP 25 µg loader
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loader
MCA2142
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F IF IP 0.2 mg loader
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loader
Search for Batch Specific Datasheets

Mouse anti Human CD63 antibody, clone MEM-259 recognizes the CD63 cell surface antigen, also known as granulophysin, lysozomal associated membrane protein 3 (LAMP-3), Melanoma-associated antigen ME491, Ocular melanoma-associated antigen or tetraspanin-30. CD63 is a 238 amino acid, tetraspanin glycoprotein of ~40-60 kDa.

CD63 is expressed by monocytes, macrophages, endothelial cells where it is a component of Weibel-Palade bodies (Vischer et al. 1993) and by a variety of cell lines. It is located intracellularly in lysosomal granules of platelets, being translocated to the surface upon activation. It may therefore by useful as a marker of platelet activation (Hamamoto et al. 1994). CD63 expression is also associated with melanoma development (Radford et al. 1997) and expression is rapidly down-regulated by progesterone in endometrial tissues (Okada et al. 1999).

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
HPB-ALL cell line.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/25 1/100
Immunofluorescence
Immunoprecipitation
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul or 100ul whole blood.

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References for CD63 antibody

  1. Casey, T.M. et al. (2007) Organelle proteomics: identification of the exocytic machinery associated with the natural killer cell secretory lysosome.
    Mol Cell Proteomics. 6 (5): 767-80.
  2. Durand-Panteix, S. et al. (2012) B7-H1, which represses EBV-immortalized B cell killing by autologous T and NK cells, is oppositely regulated by c-Myc and EBV latency III program at both mRNA and secretory lysosome levels.
    J Immunol. 189 (1): 181-90.
  3. Abdel-Latif, D. et al. (2004) Rac2 is critical for neutrophil primary granule exocytosis.
    Blood. 104: 832-9.
  4. Norling, L.V. et al. (2012) Resolvin D1 limits polymorphonuclear leukocyte recruitment to inflammatory loci: receptor-dependent actions.
    Arterioscler Thromb Vasc Biol. 32 (8): 1970-8.
  5. McKechnie, N.M. et al. (2006) Fas-ligand is stored in secretory lysosomes of ocular barrier epithelia and released with microvesicles.
    Exp Eye Res. 83: 304-14.
  6. Spring, F.A. et al. (2013) Tetraspanins CD81 and CD82 facilitate α4β1-mediated adhesion of human erythroblasts to vascular cell adhesion molecule-1.
    PLoS One. 8(5):e62654.
  7. Pliyev, B.K. (2008) Activated human neutrophils rapidly release the chemotactically active D2D3 form of the urokinase-type plasminogen activator receptor (uPAR/CD87).
    Mol Cell Biochem. 321: 111-22.
  8. Suraeva, N.M. et al. (2015) Changes in the Morphological and Immunological Characteristics of Mel Ibr Melanoma Cells in Response to Chicken Embryo Extract.
    Bull Exp Biol Med. 159 (4): 520-3.
  9. View The Latest Product References
  10. Schäfer, T. et al. (2010) A granular variant of CD63 is a regulator of repeated human mast cell degranulation.
    Allergy. 65 (10): 1242-55.
  11. Jakhria, T. et al. (2014) β2-microglobulin amyloid fibrils are nanoparticles that disrupt lysosomal membrane protein trafficking and inhibit protein degradation by lysosomes.
    J Biol Chem. 289 (52): 35781-94.
  12. Ethier, C. et al. (2016) Calcitriol Reduces Eosinophil Necrosis Which Leads to the Diminished Release of Cytotoxic Granules.
    Int Arch Allergy Immunol. 171 (2): 119-29.

RRID
AB_324562
UniProt
P08962
Entrez Gene
CD63
GO Terms
GO:0002576 platelet degranulation
GO:0005887 integral to plasma membrane
GO:0005765 lysosomal membrane
GO:0030168 platelet activation
GO:0031088 platelet dense granule membrane
GO:0031902 late endosome membrane
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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