CD62P antibody | Psel.KO.2.5

Mouse anti Human CD62P

Product Type
Monoclonal Antibody
Clone
Psel.KO.2.5
Isotype
IgG1
Specificity
CD62P

Product Code Applications Pack Size List Price Your Price Qty
MCA2418
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F IP 0.2 mg loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Mouse anti Human CD62P antibody, clone Psel.KO.2.5 recognizes the CD62P cell surface antigen, a ~140 kDa glycoprotein, also known as P-selectin.

CD62P is expressed by activated platelets and endothelial cells, and plays an important role in adhesive processes between leucocytes and endothelial cells.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Pig
Sheep
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
CD62P transfected 300.19 cells.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised CD62P knock-out mice (strain C57/B6) were fused with cells of the NS-1 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/50
Immunoprecipitation
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

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References for CD62P antibody

  1. Massaguer, A. et al. (2000) Production and characterization of monoclonal antibodies against conserved epitopes of P-selectin (CD62P).
    Tissue Antigens. 56 (2): 117-28.
  2. Massaguer, A. et al. (2003) Characterization of platelet and soluble-porcine P-selectin (CD62P).
    Vet Immunol Immunopathol. 96 (3-4): 169-81.
  3. Krajewski, S. et al. (2012) Flow cytometry analysis of porcine platelets: optimized methods for best results.
    Platelets. 23: 386-94.
  4. Johnson, C.A. Jr et al. (2011) Platelet activation in ovines undergoing sham surgery or implant of the second generation PediaFlow pediatric ventricular assist device.
    Artif Organs. 35 (6): 602-13.
  5. Tunjungputri, R.N. et al. (2016) Invasive pneumococcal disease leads to activation and hyperreactivity of platelets.
    Thromb Res. 144: 123-6.
  6. Shankarraman, V. et al. (2014) Biocompatibility Assessment of the CentriMag-Novalung Adult ECMO Circuit in a Model of Acute Pulmonary Hypertension.
    ASAIO J. 60 (4): 429-35.
  7. Chan, C.H.H. et al. (2017) Shear Stress-Induced Total Blood Trauma in Multiple Species.
    Artif Organs. 41 (10): 934-47.
  8. Johnson, C.A. Jr. et al. (2011) Platelet activation in ovines undergoing sham surgery or implant of the second generation PediaFlow pediatric ventricular assist device.
    Artif Organs. 35 (6): 602-13.
  9. View The Latest Product References

Further Reading

  1. Piriou-Guzylack, L. & Salmon, H. (2008) Membrane markers of the immune cells in swine: an update.
    Vet Res. 39 (6): 54.

Synonyms
P-SELECTIN
RRID
AB_2238947
UniProt
P16109
Entrez Gene
SELP
GO Terms
GO:0001530 lipopolysaccharide binding
GO:0002576 platelet degranulation
GO:0005887 integral to plasma membrane
GO:0005615 extracellular space
GO:0005624 membrane fraction
GO:0005625 soluble fraction
GO:0008201 heparin binding
GO:0050829 defense response to Gram-negative bacterium
GO:0009897 external side of plasma membrane
GO:0010572 positive regulation of platelet activation
GO:0030168 platelet activation
GO:0031088 platelet dense granule membrane
GO:0031092 platelet alpha granule membrane
GO:0032496 response to lipopolysaccharide
GO:0033691 sialic acid binding
GO:0042806 fucose binding
GO:0043208 glycosphingolipid binding
GO:0070492 oligosaccharide binding
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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