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CD62P antibody | Psel.KO.2.5

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Anti Human CD62P Antibody, clone Psel.KO.2.5 thumbnail image 1 Anti Human CD62P Antibody, clone Psel.KO.2.5 thumbnail image 2 Anti Human CD62P Antibody, clone Psel.KO.2.5 thumbnail image 3
Anti Human CD62P Antibody, clone Psel.KO.2.5 gallery image 1
Figure A. FITC conjugated Mouse anti Human CD61 (MCA2488F) and Pacific Blue conjugated Mouse IgG1 isotype control (MCA928PB). Figure B and C. FITC conjugated Mouse anti Human CD61 (MCA2488F) and Pacific Blue conjugated Mouse anti Human CD62P (MCA2418PB). All experiments performed on Human blood gated on single platelets, in the presence of 10% human serum. Platelets in Figure C were thrombin activated. Data acquired on the ZE5 Cell Analyzer
Anti Human CD62P Antibody, clone Psel.KO.2.5 gallery image 2
Figure A. FITC conjugated Mouse anti Human CD61 (MCA2588F) and Alexa Fluor 700 conjugated Mouse IgG1 isotype control (MCA928A700). Figure B. FITC conjugated Mouse anti Human CD61 (MCA2588F) and Alexa Fluor 700 conjugated Mouse anti Human CD62P (MCA2418A700). All experiments performed on thrombin activated Human platelets gated on platelets, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer
Anti Human CD62P Antibody, clone Psel.KO.2.5 gallery image 3
Figure A. FITC conjugated Mouse anti Human CD61 (MCA2588F) and Alexa Fluor 647 conjugated Mouse IgG1 isotype control (MCA928A647). Figure B . FITC conjugated Mouse anti Human CD61 (MCA2588F) and Alexa Fluor 647 conjugated Mouse anti Human CD62P (MCA2418A647). All experiments performed on human blood gated on single platelets activated with thrombin, in the presence of 10% Human serum. Data acquired on the ZE5 Cell Analyzer

Mouse anti Human CD62P:Alexa Fluor® 488

Mouse anti Human CD62P:Alexa Fluor® 647

Mouse anti Human CD62P:Pacific Blue®

Mouse anti Human CD62P

Mouse anti Human CD62P:RPE

Mouse anti Human CD62P:Alexa Fluor® 700

Product Type
Monoclonal Antibody
Clone
Psel.KO.2.5
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA2418A488
Datasheet
F
MSDS
100 Tests loader

MCA2418A647
Datasheet
F
MSDS
100 Tests/1ml loader

MCA2418PB
Datasheet
F
MSDS
100 Tests/1ml loader

MCA2418
Datasheet
F IP
MSDS
0.2 mg loader

MCA2418PE
Datasheet
F
MSDS
100 Tests loader

MCA2418A700
Datasheet
F
MSDS
100 Tests/1ml loader

Mouse anti Human CD62P antibody, clone Psel.KO.2.5 recognizes the CD62P cell surface antigen, a ~140 kDa glycoprotein, also known as P-selectin.

CD62P is expressed by activated platelets and endothelial cells, and plays an important role in adhesive processes between leucocytes and endothelial cells.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Pig
Sheep
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Alexa Fluor® 488 - liquid
Product Form
Purified IgG conjugated to Alexa Fluor 647 - liquid
Product Form
Purified IgG conjugated to Pacific Blue - liquid
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Product Form
Purified IgG conjugated to Alexa Fluor 700 - liquid
Reconstitution
Reconstitute with 1.0 ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Carrier Free
Yes
Immunogen
CD62P transfected 300.19 cells.
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Fusion Partners
Spleen cells from immunised CD62P knock-out mice (strain C57/B6) were fused with cells of the NS-1 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Prior to reconstitution store at +4oC.
After reconstitution store at +4oC.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use
Guarantee
18 months from date of despatch.
Guarantee
18 months from date of despatch
Guarantee
12 months from date of despatch
Guarantee
18 months from date of despatch.
Guarantee
18 months from date of reconstitution.
Guarantee
12 months from date of despatch

More Information

UniProt
P16109 Related reagents
Entrez Gene
SELP Related reagents
GO Terms
GO:0001530 lipopolysaccharide binding
GO:0002576 platelet degranulation
GO:0005887 integral to plasma membrane
GO:0005615 extracellular space
GO:0005624 membrane fraction
GO:0005625 soluble fraction
GO:0008201 heparin binding
GO:0050829 defense response to Gram-negative bacterium
GO:0009897 external side of plasma membrane
GO:0010572 positive regulation of platelet activation
GO:0030168 platelet activation
GO:0031088 platelet dense granule membrane
GO:0031092 platelet alpha granule membrane
GO:0032496 response to lipopolysaccharide
GO:0033691 sialic acid binding
GO:0042806 fucose binding
GO:0043208 glycosphingolipid binding
GO:0070492 oligosaccharide binding
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Regulatory
For research purposes only

Applications of CD62P antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Flow Cytometry Neat 1/2
Flow Cytometry Neat
Flow Cytometry Neat 1/50
Immunoprecipitation
Flow Cytometry neat
Flow Cytometry Neat
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A E WB 0.5 mg loader
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA F IF 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA F WB 0.1 mg loader
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA F IF WB 0.1 mg loader
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F F 0.5 mg loader
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P E WB 0.5 mg loader
Goat anti Mouse IgG (Fc):FITC STAR120F C F 1 mg loader
Goat anti Mouse IgG (Fc):HRP STAR120P E WB 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A F 1 ml loader
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B C E P RE WB 1 mg loader
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 F 0.5 mg loader
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 F 1 ml loader
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 C E P 0.5 mg loader
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A C E WB 1 mg loader
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P E 1 mg loader
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B F 1 mg loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Mouse IgG1 Negative Control:Alexa Fluor® 488 MCA928A488 F 100 Tests/1ml loader
Mouse IgG1 Negative Control:Alexa Fluor® 647 MCA928A647 F 100 Tests/1ml loader
Mouse IgG1 Negative Control:Pacific Blue® MCA928PB F 100 Tests/1ml loader
Mouse IgG1 Negative Control MCA928 F 100 Tests loader
Mouse IgG1 Negative Control:RPE MCA928PE F 100 Tests loader
Mouse IgG1 Negative Control:Alexa Fluor® 700 MCA928A700 F 100 Tests/1ml loader

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Human Seroblock BUF070A F 50 Test
Human Seroblock BUF070B F 200 Test
Human Seroblock BUF070A F 50 Test
Human Seroblock BUF070B F 200 Test
Human Seroblock BUF070A F 50 Test
Human Seroblock BUF070B F 200 Test
Human Seroblock BUF070A F 50 Test
Human Seroblock BUF070B F 200 Test
Human Seroblock BUF070A F 50 Test
Human Seroblock BUF070B F 200 Test

Product Specific References

References for CD62P antibody

  1. Massaguer, A. et al. (2000) Production and characterization of monoclonal antibodies against conserved epitopes of P-selectin (CD62P).
    Tissue Antigens. 56 (2): 117-28.
  2. Massaguer, A. et al. (2003) Characterization of platelet and soluble-porcine P-selectin (CD62P).
    Vet Immunol Immunopathol. 96 (3-4): 169-81.
  3. Krajewski, S. et al. (2012) Flow cytometry analysis of porcine platelets: optimized methods for best results.
    Platelets. 23: 386-94.
  4. Johnson, C.A. Jr et al. (2011) Platelet activation in ovines undergoing sham surgery or implant of the second generation PediaFlow pediatric ventricular assist device.
    Artif Organs. 35 (6): 602-13.
  5. Tunjungputri, R.N. et al. (2016) Invasive pneumococcal disease leads to activation and hyperreactivity of platelets.
    Thromb Res. 144: 123-6.
  6. Shankarraman, V. et al. (2014) Biocompatibility Assessment of the CentriMag-Novalung Adult ECMO Circuit in a Model of Acute Pulmonary Hypertension.
    ASAIO J. 60 (4): 429-35.
  7. Chan, C.H.H. et al. (2017) Shear Stress-Induced Total Blood Trauma in Multiple Species.
    Artif Organs. 41 (10): 934-47.
  8. Johnson, C.A. Jr. et al. (2011) Platelet activation in ovines undergoing sham surgery or implant of the second generation PediaFlow pediatric ventricular assist device.
    Artif Organs. 35 (6): 602-13.

Further Reading

  1. Piriou-Guzylack, L. & Salmon, H. (2008) Membrane markers of the immune cells in swine: an update.
    Vet Res. 39 (6): 54.
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