CD62E/CD62P antibody | 1.2B6
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|Mouse anti Human CD62E/CD62P antibody, clone 1.2B6 recognizes the human CD62E and CD62P cell surface antigens.
Although initially thought to recognize only human CD62E, more recent data (Goda et al. 2003) shows that Mouse anti Human CD62E/CD62P antibody, clone 1.2B6 also recognizes human CD62P, binding to a common epitope shared by these members of the selectin family.
Clone 1.2B6 reacts with porcine E-selectin (CD62E) but not with porcine P-selectin (Stocker et al. 2000).
- Target Species
- Species Cross-Reactivity
Target Species Cross Reactivity Pig
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide
- Carrier Free
- Human E-Selectin (ELAM-1).
- Approx. Protein Concentrations
- IgG concentration 1 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the NS1 mouse myeloma cell line.
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Frozen 1||1ug/ml||10ug/ml|
|Immunohistology - Paraffin|
|Western Blotting 2|
- 1The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Bio-Rad recommends the use of acetone fixation for frozen sections.
- 2Clone 1.2B6 recognizes human CD62E/CD62P only under non-reducing conditions.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
- Histology Positive Control Tissue
- Human Tonsil
References for CD62E/CD62P antibody
Wellicome, S.M. et al. (1990) A monoclonal antibody that detects a novel antigen on endothelial cells that is induced by tumor necrosis factor, IL-1, or lipopolysaccharide.
J Immunol. 144 (7): 2558-65.
Thornhill, M.H. & Haskard, D.O. (1990) IL-4 regulates endothelial cell activation by IL-1, tumor necrosis factor, or IFN-gamma.
J Immunol. 145 (3): 865-72.
Kyan-Aung, U. et al. (1991) Endothelial leukocyte adhesion molecule-1 and intercellular adhesion molecule-1 mediate the adhesion of eosinophils to endothelial cells in vitro and are expressed by endothelium in allergic cutaneous inflammation in vivo.
J Immunol. 146 (2): 521-8.
Keelan, E.T. et al. (1994) Characterization of E-selectin expression in vivo with use of a radiolabeled monoclonal antibody.
Am J Physiol. 266 (1 Pt 2): H278-90.
Goda, K. et al. (1999) Characterization of an apparently conserved epitope in E- and P-selectin identified by dual-specific monoclonal antibodies.
Eur J Immunol. 29 (5): 1551-60.
Urquhart, P. et al. (2007) Carbon monoxide-releasing molecules modulate leukocyte-endothelial interactions under flow.
J Pharmacol Exp Ther 321: 656-662.
Gómez del Moral, M. et al. (1999) African swine fever virus infection induces tumor necrosis factor alpha production: implications in pathogenesis.
J Virol. 73: 2173-80.
Vallée, I. et al. (2001) African swine fever virus infection of porcine aortic endothelial cells leads to inhibition of inflammatory responses, activation of the thrombotic state, and apoptosis.
J Virol. 75: 10372-82.
View The Latest Product References
Stocker, C.J. et al. (2000) TNF-alpha, IL-4, and IFN-gamma regulate differential expression of P- and E-selectin expression by porcine aortic endothelial cells.
J Immunol. 164: 3309-15.
Rathod, K.S. et al. (2017) Accelerated resolution of inflammation underlies sex differences in inflammatory responses in humans.
J Clin Invest. 127 (1): 169-182.
- Entrez Gene
- GO Terms
- GO:0001530 lipopolysaccharide binding
- GO:0002576 platelet degranulation
- GO:0005887 integral to plasma membrane
- GO:0005615 extracellular space
- GO:0005624 membrane fraction
- GO:0005625 soluble fraction
- GO:0008201 heparin binding
- GO:0050829 defense response to Gram-negative bacterium
- GO:0009897 external side of plasma membrane
- View More GO Terms
- GO:0010572 positive regulation of platelet activation
- GO:0030168 platelet activation
- GO:0031088 platelet dense granule membrane
- GO:0031092 platelet alpha granule membrane
- GO:0032496 response to lipopolysaccharide
- GO:0033691 sialic acid binding
- GO:0042806 fucose binding
- GO:0043208 glycosphingolipid binding
- GO:0070492 oligosaccharide binding
- GO:0007157 heterophilic cell-cell adhesion
- GO:0002092 positive regulation of receptor internalization
- GO:0002523 leukocyte migration involved in inflammatory response
- GO:0016021 integral to membrane
- GO:0004888 transmembrane receptor activity
- GO:0005901 caveola
- GO:0005905 coated pit
- GO:0007202 activation of phospholipase C activity
- GO:0019722 calcium-mediated signaling
- GO:0030029 actin filament-based process
- GO:0030863 cortical cytoskeleton
- GO:0034612 response to tumor necrosis factor
- GO:0043274 phospholipase binding
- GO:0048471 perinuclear region of cytoplasm
- GO:0050727 regulation of inflammatory response
- GO:0050901 leukocyte tethering or rolling
- GO:0070555 response to interleukin-1
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