CD59 antibody | rA49B49-05/3A2
Mouse anti Human CD59
- Product Type
- Monoclonal Antibody
- Clone
- rA49B49-05/3A2
- Isotype
- IgG1
- Specificity
- CD59
Antibody Quality and Performance Guarantee
TZA0176GA
Recombinant Antibodies
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| Mouse anti Human CD59 antibody, clone rA49B49-05/3A2, is a recombinant monoclonal antibody that recognizes CD59, also known as MAC-inhibitory protein (MAC-IP), membrane inhibitor of reactive lysis (MIRL) or protectin. CD59 is predominantly a GPI-anchored membrane protein that is widely expressed across cell and tissue types, including erythrocytes, leukocytes and various epithelial cells. It is an essential component of the innate immune system, inhibiting membrane attack complex (MAC) formation by binding the component complement proteins C8 and C9. CD59 also regulates T cell transduction by transferring a palmitate group to the linker for activation of T cells protein (LAT), causing co-localisation in lipid rafts. CD59 deficiency results in excessive complement activation and is associated with various disorders, including paroxysmal nocturnal hemoglobinuria, diabetes and multiple sclerosis. CD59 expression is elevated in multiple cancers, contributing to tumor cell evasion of the innate immune system. |
- Target Species
- Human
- Product Form
- Purified recombinant IgG containing a SpyTag at the C-terminus of the antibody heavy chain – liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Source
- CHO cells
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Recombinant CD59 Leu26-Asn102 expressed by mammalian cells
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 5μl of the suggested working dilution to label 0.5x106 cells in 100μl. Best practices suggest a 5 min centrifugation at 6,000g prior to sample application.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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| Description | Mouse IgG1 Negative Control | ||||||
- Synonyms
- MAC-IP
- UniProt
- P13987
- Entrez Gene
- CD59
- GO Terms
- GO:0005515 protein binding
- GO:0007596 blood coagulation
- GO:0005576 extracellular region
- GO:0005624 membrane fraction
- GO:0007166 cell surface receptor linked signaling pathway
- GO:0031362 anchored to external side of plasma membrane
View more products with CD59 specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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