CD59 antibody | MEM-43
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Mouse anti Human CD59
- Product Type
- Monoclonal Antibody
|Mouse anti Human CD59 antibody, clone MEM-43 recognizes CD59, a glycosyl-phosphatidylinositol (GPI) anchored membrane protein also known as membrane attack complex inhibition factor. CD59 blocks the formation of the complement membrane attack complex (MAC) by binding of C8a and C9. CD59 is found on all types of leucocytes including platelets and is also expressed on many non-haematopoietic cells.
The epitope recognized by Mouse anti Human CD59 antibody, clone MEM-43 is lost after reduction therefore, non-reducing conditions are required for western blotting techniques.
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Thymocytes and T lymphocytes.
- Approx. Protein Concentrations
- IgG concentration 1 mg/ml
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
|Application Name||Verified||Min Dilution||Max Dilution|
|Immunohistology - Frozen|
|Immunohistology - Paraffin 1|
|Western Blotting 2|
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
- 2This product recognizes CD59 under non-reducing conditions.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
- Histology Positive Control Tissue
References for CD59 antibody
Horejsí, V. et al. (1988) Monoclonal antibodies against human leucocyte antigens. II. Antibodies against CD45 (T200), CD3 (T3), CD43, CD10 (CALLA), transferrin receptor (T9), a novel broadly expressed 18-kDa antigen (MEM-43) and a novel antigen of restricted expression (MEM-74).
Folia Biol (Praha). 34 (1): 23-34.
Stefanová, I. et al. (1989) Characterization of a broadly expressed human leucocyte surface antigen MEM-43 anchored in membrane through phosphatidylinositol.
Mol Immunol. 26 (2): 153-61.
Stefanová, I. et al. (1989) in Leucocyte Typing IV: White cell differentiation antigens.
Ed. Knapp, W. et al. Oxford University Press pp 678-97.
Stefanová, I. & Horejsí, V. (1991) Association of the CD59 and CD55 cell surface glycoproteins with other membrane molecules.
J Immunol. 147 (5): 1587-92.
Tandon, N. et al. (1994) Expression and function of multiple regulators of complement activation in autoimmune thyroid disease.
Immunology. 81 (4): 643-7.
Vanderplasschen, A. et al. (1997) Extracellular enveloped vaccinia virus is resistant to complement because of incorporation of host complement control proteins into its envelope.
Proc Natl Acad Sci U S A. 95: 7544-9.
Cowan, P.J. et al. (1998) High-level endothelial expression of human CD59 prolongs heart function in an ex vivo model of xenograft rejection.
Transplantation. 65: 826-31.
Chong, Y.H. and Lee, M.J. (2000) Expression of complement inhibitor protein CD59 in human neuronal and glial cell lines treated with HIV-1 gp41 peptides.
J Neurovirol. 6: 51-60.
View The Latest Product References
Shamri, R. et al. (2002) Chemokine stimulation of lymphocyte alpha 4 integrin avidity but not of leukocyte function-associated antigen-1 avidity to endothelial ligands under shear flow requires cholesterol membrane rafts.
J Biol Chem. 277: 40027-35.
Zhang, J. et al. (2002) Early complement activation and decreased levels of glycosylphosphatidylinositol-anchored complement inhibitors in human and experimental diabetic retinopathy.
Diabetes. 51: 3499-504.
Donin, N. et al. (2003) Complement resistance of human carcinoma cells depends on membrane regulatory proteins, protein kinases and sialic acid.
Clin Exp Immunol. 131: 254-63.
Gendek-Kubiak, H. and Gendek, E.G. (2004) Immunolocalization of protectin (CD59) and macrophages in polymyositis and dermatomyositis.
J Neuroimmunol. 149: 187-94.
Jolly, C, and Sattentau. Q.J. (2005) Human Immunodeficiency Virus Type 1 Virological Synapse Formation in T Cells Requires Lipid Raft Integrity
J Virol. 79: 12088-94.
Ohyama, M. et al. (2006) Characterization and isolation of stem cell-enriched human hair follicle bulge cells.
J Clin Invest. 116: 249-60.
Ellison, B.S. et al. (2007) Complement susceptibility in glutamine deprived breast cancer cells.
Cell Div. 2007 2: 20.
Takemoto, M. et al. (2007) Human herpesvirus 7 infection increases the expression levels of CD46 and CD59 in target cells.
J Gen Virol. 88: 1415-22.
Shaw, M.L. et al. (2008) Cellular proteins in influenza virus particles.
PLoS Pathog. 4: e1000085.
Bonnon, C. et al. (2010) Selective export of human GPI-anchored proteins from the endoplasmic reticulum.
J Cell Sci. 123: 1705-15.
Sadallah, S. et al. (2011) Microparticles (ectosomes) shed by stored human platelets downregulate macrophages and modify the development of dendritic cells.
J Immunol. 186: 6543-52.
Rondelli, T. et al. (2013) The frequency of granulocytes with spontaneous somatic mutations: a wide distribution in a normal human population.
PLoS One. 8 (1): e54046.
Abe, Y. et al. (2017) Glycan region of GPI anchored-protein is required for cytocidal oligomerization of an anticancer parasporin-2, Cry46Aa1 protein, from Bacillus thuringiensis strain A1547.
J Invertebr Pathol. 142: 71-81.
Sica, M. et al. (2017) Eculizumab treatment: stochastic occurrence of C3 binding to individual PNH erythrocytes.
J Hematol Oncol. 10 (1): 126.
Gullipalli, D. et al. (2018) Antibody Inhibition of Properdin Prevents Complement-Mediated Intravascular and Extravascular Hemolysis.
J Immunol. 201 (3): 1021-1029.
Ueda, M. et al. (2019) Endovascular trophoblast expresses CD59 to evade complement-dependent cytotoxicity.
Mol Cell Endocrinol. 490: 57-67.
- Entrez Gene
- GO Terms
- GO:0005515 protein binding
- GO:0007596 blood coagulation
- GO:0005576 extracellular region
- GO:0005624 membrane fraction
- GO:0007166 cell surface receptor linked signaling pathway
- GO:0031362 anchored to external side of plasma membrane
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