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Mouse anti Human CD56

Product Type
Monoclonal Antibody
Clone
rAB03-1H8
Isotype
IgG1
Specificity
CD56

Product Code Applications Pack Size List Price Your Price Qty
TZA062GA
Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.1 mg loader
List Price Your Price
loader

Mouse anti Human CD56 antibody, clone rAB03-1H8, is a recombinant monoclonal antibody that recognizes CD56, also known as human neural cell adhesion molecule (NCAM).

CD56 is a cell surface glycoprotein of the immunoglobulin superfamily. In the human hematopoietic system, CD56 is expressed on natural killer (NK) cells, alpha beta T cells, gamma delta T cells, dendritic cells and monocytes. As the name suggests, it is also expressed by cells of neuronal lineage. Three main isoforms of CD56 exist that differ in the intracellular domain. In NK cells, CD56 has been shown to act as a pathogen recognition receptor, promote cytotoxicity and stimulate exocytosis. In neuronal tissues, CD56 mediates homophilic and heterophilic adhesion, and is implicated in neural development, cell migration and synaptic plasticity.

CD56 is considered a classical surface marker of NK cells, which are usually divided into two subsets, CD56dim and CD56bright. NK cells in peripheral blood are predominantly CD56dim, whilst CD56bright NK cells are more prevalent in tissues. Expression of CD56 by NK cells can be used as an activation marker. Aberrant CD56 expression is associated with a range of tumors and malignancies, including tumors of the lung, neural derived malignancies, and natural killer cell lymphomas.

Target Species
Human
Product Form
Purified recombinant IgG containing a SpyTag at the C-terminus of the antibody heavy chain – liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Source
CHO cells
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Recombinant CD56 Leu20-Ala708 expressed by mammalian cells
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 5μl of the suggested working dilution to label 0.5x106 cells in 100μl. Best practices suggest a 5 min centrifugation at 6,000g prior to sample application.

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Description Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)
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Description Rabbit F(ab')2 anti Mouse IgG:FITC

Description Product Code Applications Pack Size List Price Your Price Quantity
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Description Mouse IgG1 Negative Control

Synonyms
NCAM-1
UniProt
P13591
Entrez Gene
NCAM1

TZA062GA

PDF 64684036

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