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CD49b antibody | 31H4

Product Code Applications Pack Size List Price Your Price Qty
MCA2027
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E F IP 0.2 mg loader
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loader

Mouse anti Human CD49b antibody, clone 31H4 recognizes human CD49b also known as integrin alpha-2, VLA-2 subunit alpha or collogen receptor. CD49b is a 1181 amino acid ¦130 kDa single pass type-1 transmembrane glycoprotein which forms a heterodimer with integrin beta-1.

Mouse anti Human CD49b antibody, clone 31H4 has been identified as being capable of immunoprecipitating the integrin a2 subunit from human cell-lines surface labelled with 125I. It has been confirmed specific to a2 by relative expression of antigen on various cell lines by flow cytometry, and recognition of affinity purified a2b1 in dot blots.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
Carrier Free
Yes
Immunogen
Purified human b1 integrin preparation from HT1080 fibrosarcoma cell extract.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the X63/Ag8.653 mouse myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 10ug/ml
Flow Cytometry 1/25 1/50
Immunoprecipitation 25ug/ml 50ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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References for CD49b antibody

  1. Tanaka, T. et al. (2009) Remodeling of the human endometrial epithelium is regulated by laminin and type IV collagen.
    Int J Mol Med. 23: 173-80.
  2. Tanaka, T. et al. (2005) Reduced radiosensitivity and increased CD40 expression in cyclophosphamide-resistant subclones established from human cervical squamous cell carcinoma cells.
    Oncol Rep. 14: 941-8.
  3. Tanaka, T. et al. (2010) Establishment and characterization of novel human uterine leiomyosarcoma cell lines
    Int J Oncol. 37: 125-31.
  4. Tanaka, T. et al. (2012) Differential sensitivity to paclitaxel-induced apoptosis and growth suppression in paclitaxel-resistant cell lines established from HEC-1 human endometrial adenocarcinoma cells.
    Int J Oncol. 41: 1837-44.
  5. Tanaka, T. et al. (2008) Autocrine/paracrine regulation of human endometrial stromal remodeling by laminin and type IV collagen.
    Int J Mol Med. 22: 581-7.
  6. Tanaka, T. et al. (2006) Optimal combination chemotherapy and chemoradiotherapy with etoposide for advanced cervical squamous cancer cells in vitro.
    Oncol Rep. 15: 939-47.
  7. Tanaka, T. et al. (2012) Impaired death-associated protein kinase-mediated survival signals in 5-fluorouracil-resistant human endometrial adenocarcinoma cells.
    Oncol Rep. 28 (1): 330-6.
  8. Tanaka, T. et al. (2008) Experimental characterization of recurrent ovarian immature teratoma cells after optimal surgery.
    Oncol Rep. 20: 13-23.
  9. View The Latest Product References

Synonyms
Integrin Alpha 2 Chain
VLA-2
RRID
AB_321445
UniProt
P17301
Entrez Gene
ITGA2
GO Terms
GO:0007596 blood coagulation
GO:0004872 receptor activity
GO:0005518 collagen binding
GO:0008305 integrin complex
GO:0007160 cell-matrix adhesion
GO:0007229 integrin-mediated signaling pathway
GO:0007411 axon guidance
GO:0042802 identical protein binding
GO:0044419 interspecies interaction between organisms

MCA2027

158555 1601

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