CD49b antibody | 31H4

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Mouse anti Human CD49b

Product Type: Monoclonal Antibody
Clone: 31H4
Isotype: IgG1

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
MCA2027	E F IP			0.2 mg

Mouse anti Human CD49b antibody, clone 31H4 recognizes human CD49b also known as integrin alpha-2, VLA-2 subunit alpha or collogen receptor. CD49b is a 1181 amino acid ¦130 kDa single pass type-1 transmembrane glycoprotein which forms a heterodimer with integrin beta-1.

Mouse anti Human CD49b antibody, clone 31H4 has been identified as being capable of immunoprecipitating the integrin a2 subunit from human cell-lines surface labelled with ¹²⁵I. It has been confirmed specific to a2 by relative expression of antigen on various cell lines by flow cytometry, and recognition of affinity purified a2b1 in dot blots.

Product Details

Target Species

Human

Species Cross-Reactivity

Target Species	Cross Reactivity
Rat

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Preparation

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09%

Sodium Azide

Carrier Free

Yes

Immunogen

Purified human b1 integrin preparation from HT1080 fibrosarcoma cell extract.

Approx. Protein Concentrations

IgG concentration 1.0 mg/ml

Fusion Partners

Spleen cells from immunised BALB/c mice were fused with cells of the X63/Ag8.653 mouse myeloma cell line.

Storage Information

Storage: Store at +4^oC for one month or at -20^oC for longer.

This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

More Information

UniProt: P17301 Related reagents
Entrez Gene: ITGA2 Related reagents
GO Terms: GO:0007596 blood coagulation; GO:0004872 receptor activity; GO:0005518 collagen binding; GO:0008305 integrin complex; GO:0007160 cell-matrix adhesion; GO:0007229 integrin-mediated signaling pathway; GO:0007411 axon guidance; GO:0042802 identical protein binding; GO:0044419 interspecies interaction between organisms
Regulatory: For research purposes only

Applications of CD49b antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
ELISA		10ug/ml
Flow Cytometry	1/25	1/50
Immunoprecipitation	25ug/ml	50ug/ml

Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Negative Isotype Controls Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Mouse IgG1 Negative Control	MCA928	100 Tests	F

Product Specific References

References for CD49b antibody

Tanaka, T. et al. (2009) Remodeling of the human endometrial epithelium is regulated by laminin and type IV collagen.
Int J Mol Med. 23: 173-80.
Tanaka, T. et al. (2005) Reduced radiosensitivity and increased CD40 expression in cyclophosphamide-resistant subclones established from human cervical squamous cell carcinoma cells.
Oncol Rep. 14: 941-8.
Tanaka, T. et al. (2010) Establishment and characterization of novel human uterine leiomyosarcoma cell lines
Int J Oncol. 37: 125-31.
Tanaka, T. et al. (2012) Differential sensitivity to paclitaxel-induced apoptosis and growth suppression in paclitaxel-resistant cell lines established from HEC-1 human endometrial adenocarcinoma cells.
Int J Oncol. 41: 1837-44.
Tanaka, T. et al. (2008) Autocrine/paracrine regulation of human endometrial stromal remodeling by laminin and type IV collagen.
Int J Mol Med. 22: 581-7.
Tanaka, T. et al. (2006) Optimal combination chemotherapy and chemoradiotherapy with etoposide for advanced cervical squamous cancer cells in vitro.
Oncol Rep. 15: 939-47.
Tanaka, T. et al. (2012) Impaired death-associated protein kinase-mediated survival signals in 5-fluorouracil-resistant human endometrial adenocarcinoma cells.
Oncol Rep. 28: 330-6.
Tanaka, T. et al. (2008) Experimental characterization of recurrent ovarian immature teratoma cells after optimal surgery.
Oncol Rep. 20: 13-23.

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