Mouse anti Human CD49b antibody, clone 31H4 recognizes human CD49b also known as integrin alpha-2, VLA-2 subunit alpha or collogen receptor. CD49b is a 1181 amino acid ¦130 kDa single pass type-1 transmembrane glycoprotein which forms a heterodimer with integrin beta-1.
Mouse anti Human CD49b antibody, clone 31H4 has been identified as being capable of immunoprecipitating the integrin a2 subunit from human cell-lines surface labelled with 125I. It has been confirmed specific to a2 by relative expression of antigen on various cell lines by flow cytometry, and recognition of affinity purified a2b1 in dot blots.
- Target Species
- Species Cross-Reactivity
|Target Species||Cross Reactivity|
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- Carrier Free
- Purified human b1 integrin preparation from HT1080 fibrosarcoma cell extract.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the X63/Ag8.653 mouse myeloma cell line.
- Store at +4oC for one month or at -20oC for longer.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
integrin-mediated signaling pathway
identical protein binding
interspecies interaction between organisms
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of CD49b antibody
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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Secondary Antibodies Available
Negative Isotype Controls Available
Product Specific References
References for CD49b antibody
Tanaka, T. et al. (2009) Remodeling of the human endometrial epithelium is regulated by laminin and type IV collagen.
Int J Mol Med. 23: 173-80.
Tanaka, T. et al. (2005) Reduced radiosensitivity and increased CD40 expression in cyclophosphamide-resistant subclones established from human cervical squamous cell carcinoma cells.
Oncol Rep. 14: 941-8.
Tanaka, T. et al. (2010) Establishment and characterization of novel human uterine leiomyosarcoma cell lines
Int J Oncol. 37: 125-31.
Tanaka, T. et al. (2012) Differential sensitivity to paclitaxel-induced apoptosis and growth suppression in paclitaxel-resistant cell lines established from HEC-1 human endometrial adenocarcinoma cells.
Int J Oncol. 41: 1837-44.
Tanaka, T. et al. (2008) Autocrine/paracrine regulation of human endometrial stromal remodeling by laminin and type IV collagen.
Int J Mol Med. 22: 581-7.
Tanaka, T. et al. (2006) Optimal combination chemotherapy and chemoradiotherapy with etoposide for advanced cervical squamous cancer cells in vitro.
Oncol Rep. 15: 939-47.
Tanaka, T. et al. (2012) Impaired death-associated protein kinase-mediated survival signals in 5-fluorouracil-resistant human endometrial adenocarcinoma cells.
Oncol Rep. 28: 330-6.
Tanaka, T. et al. (2008) Experimental characterization of recurrent ovarian immature teratoma cells after optimal surgery.
Oncol Rep. 20: 13-23.
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