CD49b antibody | 16B4


Mouse anti Human CD49b:Alexa Fluor® 488
Mouse anti Human CD49b
Mouse anti Human CD49b:RPE
- Product Type
- Monoclonal Antibody
- Clone
- 16B4
- Isotype
- IgG1
Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|
MCA2025A488 | F | 100 Tests/1ml |
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MCA2025T | C E F FN* IF IP P* WB | 25 µg |
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MCA2025 | C E F FN* IF IP P* WB | 0.2 mg |
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MCA2025PE | F | 100 Tests |
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Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Alexa Fluor 488 - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
- Reconstitution
- Reconstitute with 1.0 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. - Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Preservative Stabilisers
0.09% Sodium Azide - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose - Carrier Free
- Yes
- Immunogen
- Purified human beta 1 preparation from HT1080 fibrosarcoma cell extract
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the X63/Ag8.653 mouse myeloma cell line
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Prior to reconstitution store at +4oC.
After reconstitution store at +4oC.
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. - Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P17301
- Entrez Gene
- ITGA2
- GO Terms
- GO:0007596 blood coagulation
- GO:0004872 receptor activity
- GO:0005518 collagen binding
- GO:0008305 integrin complex
- GO:0007160 cell-matrix adhesion
- GO:0007229 integrin-mediated signaling pathway
- GO:0007411 axon guidance
- GO:0042802 identical protein binding
- GO:0044419 interspecies interaction between organisms
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
- Regulatory
- For research purposes only
Applications of CD49b antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | Neat | ||
ELISA | 10ug/ml | ||
Flow Cytometry | 30ug/ml | ||
Functional Assays 1 | |||
Immunofluorescence | |||
Immunohistology - Frozen | 30ug/ml | 40ug/ml | |
Immunohistology - Paraffin 2 | |||
Immunoprecipitation | 25ug/ml | 50ug/ml | |
Western Blotting | |||
Flow Cytometry | Neat |
- 1This product contains sodium azide, removal by dialysis is recommended prior to use in functional assays.
- 2This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.
Tris/EDTA pH9.0 is recommended for this purpose.
Secondary Antibodies Available
Negative Isotype Controls Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Mouse IgG1 Negative Control:Alexa Fluor® 488 | MCA928A488 | F | 100 Tests/1ml |
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Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
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Mouse IgG1 Negative Control:RPE | MCA928PE | F | 100 Tests |
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Useful Reagents Available
Description | Product Code | Applications | Pack Size | List Price | Quantity |
---|---|---|---|---|---|
Human Seroblock | BUF070A | F | 50 Test | ||
Human Seroblock | BUF070B | F | 200 Test | ||
Human Seroblock | BUF070A | F | 50 Test | ||
Human Seroblock | BUF070B | F | 200 Test |
Application Based External Images
ELISA
Flow Cytometry
Functional Assays
Immunofluorescence
Western Blotting
Product Specific References
References for CD49b antibody
-
Fitter, S. et al. (1999) Transmembrane 4 superfamily protein CD151 (PETA-3) associates with beta 1 and alpha IIb beta 3 integrins in haemopoietic cell lines and modulates cell-cell adhesion.
Biochem J. 338 ( Pt 1): 61-70. -
Sincock, P.M. et al. (1999) PETA-3/CD151, a member of the transmembrane 4 superfamily, is localised to the plasma membrane and endocytic system of endothelial cells, associates with multiple integrins and modulates cell function.
J Cell Sci. 112 ( Pt 6): 833-44. -
Eaton, C.L. et al. (2010) Evaluation of the frequency of putative prostate cancer stem cells in primary and metastatic prostate cancer.
Prostate. 70 (8): 875-82. -
Gassmann, P. et al. (2009) CXCR4 regulates the early extravasation of metastatic tumor cells in vivo.
Neoplasia. 11: 651-61. -
Gassmann, P. et al. (2010) In vivo tumor cell adhesion in the pulmonary microvasculature is exclusively mediated by tumor cell--endothelial cell interaction.
BMC Cancer. 10: 177. -
Ivaska, J. et al. (2005) PKCepsilon-mediated phosphorylation of vimentin controls integrin recycling and motility.
EMBO J. 24: 3834-45. -
Pellinen, T. et al. (2006) Small GTPase Rab21 regulates cell adhesion and controls endosomal traffic of beta1-integrins.
J Cell Biol. 173: 767-80. -
Upla, P. et al. (2004) Clustering induces a lateral redistribution of alpha 2 beta 1 integrin from membrane rafts to caveolae and subsequent protein kinase C-dependent internalization.
Mol Biol Cell. 15: 625-36. -
Jokinen, J. et al. (2010) Molecular mechanism of alpha2beta1 integrin interaction with human echovirus 1.
EMBO J. 29: 196-208. -
Offterdinger, M. et al. (2003) Heregulin and retinoids synergistically induce branching morphogenesis of breast cancer cells cultivated in 3D collagen gels.
J Cell Physiol. 195: 260-75. -
Connors, W.L. and Heino, J. (2005) A duplexed microsphere-based cellular adhesion assay.
Anal Biochem. 337: 246-55. -
Connors, W.L. et al. (2007) Two synergistic activation mechanisms of alpha2beta1 integrin-mediated collagen binding.
J Biol Chem. 282: 14675-83. -
Vuoriluoto, K. et al. (2008) Syndecan-1 supports integrin alpha2beta1-mediated adhesion to collagen.
Exp Cell Res. 314: 3369-81. -
Holleran, B.J. et al. (2003) Differential recruitment of alpha2beta1 and alpha4beta1 integrins to lipid rafts in Jurkat T lymphocytes exposed to collagen type IV and fibronectin.
J Leukoc Biol. 73: 243-52. -
Rintanen, N. et al. (2012) Calpains promote α2β1 integrin turnover in nonrecycling integrin pathway.
Mol Biol Cell. 23: 448-463. -
Pietiäinen, V. et al. (2004) Echovirus 1 endocytosis into caveosomes requires lipid rafts, dynamin II, and signaling events.
Mol Biol Cell. 15: 4911-25. -
Kankaanpää, P. et al. (2015) Cellular recognition and macropinocytosis-like internalization of nanoparticles targeted to integrin α2β1.
Nanoscale. 7 (42): 17889-901. -
Poulter, N.S. et al. (2017) Clustering of glycoprotein VI (GPVI) dimers upon adhesion to collagen as a mechanism to regulate GPVI signaling in platelets.
J Thromb Haemost. 15 (3): 549-564.
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