CD49b antibody | 16B4
Mouse anti Human CD49b
- Product Type
- Monoclonal Antibody
- Clone
- 16B4
- Isotype
- IgG1
- Specificity
- CD49b
Antibody Quality and Performance Guarantee
| Mouse anti Human CD49b antibody, clone 16B4 recognizes human CD49b, also known as integrin α2, collagen receptor, Platelet membrane glycoprotein Ia or VLA-2 subunit alpha. CD49b is a 1181 amino acid ~160 kDa single pass type-1 transmembrane glycoprotein possessing multiple FG-GAP repeats and a single VWFA domain. Mouse anti human CD49b antibody, clone 16B4 is identified as capable of immunoprecipitating a non-reducible alpha subunit from I125 surface labeled human cell line extract. It is confirmed as specific to the alpha 2 subunit by relative expression of antigen on various cell lines by FACS, and its recognition of affinity purified alpha 2 beta 1 in dot blots. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Purified human beta 1 preparation from HT1080 fibrosarcoma cell extract
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the X63/Ag8.653 mouse myeloma cell line
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
10ug/ml | |
| Flow Cytometry | |
30ug/ml | |
| Functional Assays 1 | |
||
| Immunofluorescence | |
||
| Immunohistology - Frozen | |
30ug/ml | 40ug/ml |
| Immunohistology - Paraffin 2 | |
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| Immunoprecipitation | |
25ug/ml | 50ug/ml |
| Western Blotting | |
- 1This product contains sodium azide, removal by dialysis is recommended prior to use in functional assays.
- 2This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.
Tris/EDTA pH9.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control | MCA928 | F | 100 Tests |
|
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| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control | ||||||
References for CD49b antibody
-
Fitter, S. et al. (1999) Transmembrane 4 superfamily protein CD151 (PETA-3) associates with beta 1 and alpha IIb beta 3 integrins in haemopoietic cell lines and modulates cell-cell adhesion.
Biochem J. 338 ( Pt 1): 61-70. -
Sincock, P.M. et al. (1999) PETA-3/CD151, a member of the transmembrane 4 superfamily, is localised to the plasma membrane and endocytic system of endothelial cells, associates with multiple integrins and modulates cell function.
J Cell Sci. 112 ( Pt 6): 833-44. -
Eaton, C.L. et al. (2010) Evaluation of the frequency of putative prostate cancer stem cells in primary and metastatic prostate cancer.
Prostate. 70 (8): 875-82. -
Gassmann, P. et al. (2009) CXCR4 regulates the early extravasation of metastatic tumor cells in vivo.
Neoplasia. 11: 651-61. -
Gassmann, P. et al. (2010) In vivo tumor cell adhesion in the pulmonary microvasculature is exclusively mediated by tumor cell--endothelial cell interaction.
BMC Cancer. 10: 177. -
Ivaska, J. et al. (2005) PKCepsilon-mediated phosphorylation of vimentin controls integrin recycling and motility.
EMBO J. 24: 3834-45. -
Pellinen, T. et al. (2006) Small GTPase Rab21 regulates cell adhesion and controls endosomal traffic of beta1-integrins.
J Cell Biol. 173: 767-80. -
Upla, P. et al. (2004) Clustering induces a lateral redistribution of alpha 2 beta 1 integrin from membrane rafts to caveolae and subsequent protein kinase C-dependent internalization.
Mol Biol Cell. 15: 625-36.
View The Latest Product References
-
Jokinen, J. et al. (2010) Molecular mechanism of alpha2beta1 integrin interaction with human echovirus 1.
EMBO J. 29: 196-208. -
Offterdinger, M. et al. (2003) Heregulin and retinoids synergistically induce branching morphogenesis of breast cancer cells cultivated in 3D collagen gels.
J Cell Physiol. 195: 260-75. -
Connors, W.L. and Heino, J. (2005) A duplexed microsphere-based cellular adhesion assay.
Anal Biochem. 337: 246-55. -
Connors, W.L. et al. (2007) Two synergistic activation mechanisms of alpha2beta1 integrin-mediated collagen binding.
J Biol Chem. 282: 14675-83. -
Vuoriluoto, K. et al. (2008) Syndecan-1 supports integrin alpha2beta1-mediated adhesion to collagen.
Exp Cell Res. 314: 3369-81. -
Holleran, B.J. et al. (2003) Differential recruitment of alpha2beta1 and alpha4beta1 integrins to lipid rafts in Jurkat T lymphocytes exposed to collagen type IV and fibronectin.
J Leukoc Biol. 73: 243-52. -
Rintanen, N. et al. (2012) Calpains promote α2β1 integrin turnover in nonrecycling integrin pathway.
Mol Biol Cell. 23: 448-463. -
Pietiäinen, V. et al. (2004) Echovirus 1 endocytosis into caveosomes requires lipid rafts, dynamin II, and signaling events.
Mol Biol Cell. 15: 4911-25. -
Kankaanpää, P. et al. (2015) Cellular recognition and macropinocytosis-like internalization of nanoparticles targeted to integrin α2β1.
Nanoscale. 7 (42): 17889-901. -
Poulter, N.S. et al. (2017) Clustering of glycoprotein VI (GPVI) dimers upon adhesion to collagen as a mechanism to regulate GPVI signaling in platelets.
J Thromb Haemost. 15 (3): 549-564.
- Synonyms
- Integrin Alpha 2 Chain
- VLA-2
- RRID
- AB_321444
- UniProt
- P17301
- Entrez Gene
- ITGA2
- GO Terms
- GO:0007596 blood coagulation
- GO:0004872 receptor activity
- GO:0005518 collagen binding
- GO:0008305 integrin complex
- GO:0007160 cell-matrix adhesion
- GO:0007229 integrin-mediated signaling pathway
- GO:0007411 axon guidance
- GO:0042802 identical protein binding
- GO:0044419 interspecies interaction between organisms
- View More GO Terms
MCA2025
149490
MCA2025T
157090
1702
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