CD49b antibody | 16B4
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|Mouse anti Human CD49b antibody, clone 16B4 recognizes human CD49b, also known as integrin α2, collagen receptor, Platelet membrane glycoprotein Ia or VLA-2 subunit alpha. CD49b is a 1181 amino acid ~160 kDa single pass type-1 transmembrane glycoprotein possessing multiple FG-GAP repeats and a single VWFA domain. Mouse anti human CD49b antibody, clone 16B4 is identified as capable of immunoprecipitating a non-reducible alpha subunit from I125 surface labeled human cell line extract. It is confirmed as specific to the alpha 2 subunit by relative expression of antigen on various cell lines by FACS, and its recognition of affinity purified alpha 2 beta 1 in dot blots.|
- Target Species
- Species Cross-Reactivity
Target Species Cross Reactivity Rat
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide
- Carrier Free
- Purified human beta 1 preparation from HT1080 fibrosarcoma cell extract
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the X63/Ag8.653 mouse myeloma cell line
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
|Application Name||Verified||Min Dilution||Max Dilution|
|Functional Assays 1|
|Immunohistology - Frozen||30ug/ml||40ug/ml|
|Immunohistology - Paraffin 2|
- 1This product contains sodium azide, removal by dialysis is recommended prior to use in functional assays.
- 2This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.
Tris/EDTA pH9.0 is recommended for this purpose.
References for CD49b antibody
Fitter, S. et al. (1999) Transmembrane 4 superfamily protein CD151 (PETA-3) associates with beta 1 and alpha IIb beta 3 integrins in haemopoietic cell lines and modulates cell-cell adhesion.
Biochem J. 338 ( Pt 1): 61-70.
Sincock, P.M. et al. (1999) PETA-3/CD151, a member of the transmembrane 4 superfamily, is localised to the plasma membrane and endocytic system of endothelial cells, associates with multiple integrins and modulates cell function.
J Cell Sci. 112 ( Pt 6): 833-44.
Eaton, C.L. et al. (2010) Evaluation of the frequency of putative prostate cancer stem cells in primary and metastatic prostate cancer.
Prostate. 70 (8): 875-82.
Gassmann, P. et al. (2009) CXCR4 regulates the early extravasation of metastatic tumor cells in vivo.
Neoplasia. 11: 651-61.
Gassmann, P. et al. (2010) In vivo tumor cell adhesion in the pulmonary microvasculature is exclusively mediated by tumor cell--endothelial cell interaction.
BMC Cancer. 10: 177.
Ivaska, J. et al. (2005) PKCepsilon-mediated phosphorylation of vimentin controls integrin recycling and motility.
EMBO J. 24: 3834-45.
Pellinen, T. et al. (2006) Small GTPase Rab21 regulates cell adhesion and controls endosomal traffic of beta1-integrins.
J Cell Biol. 173: 767-80.
Upla, P. et al. (2004) Clustering induces a lateral redistribution of alpha 2 beta 1 integrin from membrane rafts to caveolae and subsequent protein kinase C-dependent internalization.
Mol Biol Cell. 15: 625-36.
View The Latest Product References
Jokinen, J. et al. (2010) Molecular mechanism of alpha2beta1 integrin interaction with human echovirus 1.
EMBO J. 29: 196-208.
Offterdinger, M. et al. (2003) Heregulin and retinoids synergistically induce branching morphogenesis of breast cancer cells cultivated in 3D collagen gels.
J Cell Physiol. 195: 260-75.
Connors, W.L. and Heino, J. (2005) A duplexed microsphere-based cellular adhesion assay.
Anal Biochem. 337: 246-55.
Connors, W.L. et al. (2007) Two synergistic activation mechanisms of alpha2beta1 integrin-mediated collagen binding.
J Biol Chem. 282: 14675-83.
Vuoriluoto, K. et al. (2008) Syndecan-1 supports integrin alpha2beta1-mediated adhesion to collagen.
Exp Cell Res. 314: 3369-81.
Holleran, B.J. et al. (2003) Differential recruitment of alpha2beta1 and alpha4beta1 integrins to lipid rafts in Jurkat T lymphocytes exposed to collagen type IV and fibronectin.
J Leukoc Biol. 73: 243-52.
Rintanen, N. et al. (2012) Calpains promote α2β1 integrin turnover in nonrecycling integrin pathway.
Mol Biol Cell. 23: 448-463.
Pietiäinen, V. et al. (2004) Echovirus 1 endocytosis into caveosomes requires lipid rafts, dynamin II, and signaling events.
Mol Biol Cell. 15: 4911-25.
Kankaanpää, P. et al. (2015) Cellular recognition and macropinocytosis-like internalization of nanoparticles targeted to integrin α2β1.
Nanoscale. 7 (42): 17889-901.
Poulter, N.S. et al. (2017) Clustering of glycoprotein VI (GPVI) dimers upon adhesion to collagen as a mechanism to regulate GPVI signaling in platelets.
J Thromb Haemost. 15 (3): 549-564.
- Integrin Alpha 2 Chain
- Entrez Gene
- GO Terms
- GO:0007596 blood coagulation
- GO:0004872 receptor activity
- GO:0005518 collagen binding
- GO:0008305 integrin complex
- GO:0007160 cell-matrix adhesion
- GO:0007229 integrin-mediated signaling pathway
- GO:0007411 axon guidance
- GO:0042802 identical protein binding
- GO:0044419 interspecies interaction between organisms
- View More GO Terms
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