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CD49b antibody | 16B4

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Anti Human CD49b Antibody, clone 16B4 gallery image 1
Figure A. FITC conjugated mouse anti human CD61 (MCA2588F) and RPE conjugated mouse IgG1 isotype control (MCA928PE) Figure B. FITC conjugated mouse anti human CD61 (MCA2588F) and RPE conjugated mouse anti human CD49b (MCA2025PE). All experiments performed on human blood gated on platelets. Data acquired on the ZE5™ Cell Analyzer

Mouse anti Human CD49b

Mouse anti Human CD49b:RPE

Product Type
Monoclonal Antibody
Clone
16B4
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2025T C E F FN* IF IP P* WB 25 µg
MCA2025 C E F FN* IF IP P* WB 0.2 mg
MCA2025PE F 100 Tests
Mouse anti Human CD49b antibody, clone 16B4 recognizes human CD49b, also known as integrin α2, collagen receptor, Platelet membrane glycoprotein Ia or VLA-2 subunit alpha. CD49b is a 1181 amino acid ~160kDa single pass type-1 transmembrane glycoprotein posessing multiple FG-GAP repeats and a single VWFA domain. Mouse anti human CD49b antibody, clone 16B4 is identified as capable of immunoprecipitating a non-reducible alpha subunit from I125 surface labelled Human cell line extract. It is confirmed as specific to the alpha 2 subunit by relative expression of antigen on various cell lines by FACS, and its recognition of affinity purified alpha 2 beta 1 in dot blots.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1.0 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose
Carrier Free
Yes
Immunogen
Purified human beta 1 preparation from HT1080 fibrosarcoma cell extract.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the X63/Ag8.653 mouse myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Prior to reconstitution store at +4oC.
After reconstitution store at +4oC.
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light.
Shelf Life
18 months from date of despatch.
Shelf Life
12 months from date of reconstitution

More Information

UniProt
P17301 Related reagents
Entrez Gene
ITGA2 Related reagents
GO Terms
GO:0007596 blood coagulation
GO:0004872 receptor activity
GO:0005518 collagen binding
GO:0008305 integrin complex
GO:0007160 cell-matrix adhesion
GO:0007229 integrin-mediated signaling pathway
GO:0007411 axon guidance
GO:0042802 identical protein binding
GO:0044419 interspecies interaction between organisms
Regulatory
For research purposes only

Applications of CD49b antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 10ug/ml
Flow Cytometry 30ug/ml
Functional Assays 1
Immunofluorescence
Immunohistology - Frozen 30ug/ml 40ug/ml
Immunohistology - Paraffin 2
Immunoprecipitation 25ug/ml 50ug/ml
Western Blotting
Flow Cytometry Neat
  1. 1This product contains sodium azide, removal by dialysis is recommended prior to use in functional assays. Dialysis cassettes EQU003 are suitable for this purpose.
  2. 2This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.

    Tris/EDTA pH9.0 is recommended for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG1 Negative Control MCA928 100 Tests F
Mouse IgG1 Negative Control:RPE MCA928PE 100 Tests F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F

Application Based External Images

ELISA

Flow Cytometry

Functional Assays

Immunofluorescence

Western Blotting

Product Specific References

References for CD49b antibody

  1. Fitter, S. et al. (1999) Transmembrane 4 superfamily protein CD151 (PETA-3) associates with beta 1 and alpha IIb beta 3 integrins in haemopoietic cell lines and modulates cell-cell adhesion.
    Biochem J. 338 ( Pt 1): 61-70.
  2. Sincock, P.M. et al. (1999) PETA-3/CD151, a member of the transmembrane 4 superfamily, is localised to the plasma membrane and endocytic system of endothelial cells, associates with multiple integrins and modulates cell function.
    J Cell Sci. 112 ( Pt 6): 833-44.
  3. Eaton, C.L. et al. (2010) Evaluation of the frequency of putative prostate cancer stem cells in primary and metastatic prostate cancer.
    Prostate. 70 (8): 875-82.
  4. Gassmann, P. et al. (2009) CXCR4 regulates the early extravasation of metastatic tumor cells in vivo.
    Neoplasia. 11: 651-61.
  5. Gassmann, P. et al. (2010) In vivo tumor cell adhesion in the pulmonary microvasculature is exclusively mediated by tumor cell--endothelial cell interaction.
    BMC Cancer. 10: 177.
  6. Ivaska, J. et al. (2005) PKCepsilon-mediated phosphorylation of vimentin controls integrin recycling and motility.
    EMBO J. 24: 3834-45.
  7. Pellinen, T. et al. (2006) Small GTPase Rab21 regulates cell adhesion and controls endosomal traffic of beta1-integrins.
    J Cell Biol. 173: 767-80.
  8. Upla, P. et al. (2004) Clustering induces a lateral redistribution of alpha 2 beta 1 integrin from membrane rafts to caveolae and subsequent protein kinase C-dependent internalization.
    Mol Biol Cell. 15: 625-36.
  9. Jokinen, J. et al. (2010) Molecular mechanism of alpha2beta1 integrin interaction with human echovirus 1.
    EMBO J. 29: 196-208.
  10. Offterdinger, M. et al. (2003) Heregulin and retinoids synergistically induce branching morphogenesis of breast cancer cells cultivated in 3D collagen gels.
    J Cell Physiol. 195: 260-75.
  11. Connors, W.L. and Heino, J. (2005) A duplexed microsphere-based cellular adhesion assay.
    Anal Biochem. 337: 246-55.
  12. Connors, W.L. et al. (2007) Two synergistic activation mechanisms of alpha2beta1 integrin-mediated collagen binding.
    J Biol Chem. 282: 14675-83.
  13. Vuoriluoto, K. et al. (2008) Syndecan-1 supports integrin alpha2beta1-mediated adhesion to collagen.
    Exp Cell Res. 314: 3369-81.
  14. Holleran, B.J. et al. (2003) Differential recruitment of alpha2beta1 and alpha4beta1 integrins to lipid rafts in Jurkat T lymphocytes exposed to collagen type IV and fibronectin.
    J Leukoc Biol. 73: 243-52.
  15. Rintanen, N. et al. (2012) Calpains promote α2β1 integrin turnover in nonrecycling integrin pathway.
    Mol Biol Cell. 23: 448-463.
  16. Pietiäinen, V. et al. (2004) Echovirus 1 endocytosis into caveosomes requires lipid rafts, dynamin II, and signaling events.
    Mol Biol Cell. 15: 4911-25.
  17. Kankaanpää, P. et al. (2015) Cellular recognition and macropinocytosis-like internalization of nanoparticles targeted to integrin α2β1.
    Nanoscale. 7 (42): 17889-901.
  18. Poulter, N.S. et al. (2017) Clustering of glycoprotein VI (GPVI) dimers upon adhesion to collagen as a mechanism to regulate GPVI signaling in platelets.
    J Thromb Haemost. 15 (3): 549-564.
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